Disturbances to neurotransmitter levels and their metabolic enzyme activity in a freshwater planarian exposed to cadmium

Wu, Jui-Pin; Li, Mei-Hui; Chen, Jhih-Sheng; Chung, Szu-Yao; Lee, Hui‐Ling

doi:10.1016/j.neuro.2015.01.003

Cited by 21 publications

(4 citation statements)

References 44 publications

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“…The downregulation of MAO leads to the decrease of DA metabolism ( Graves et al, 2020 ). MAO is the essential metabolic enzyme to DA metabolism and catalyzes 5-HT to 5-HIAA ( Wu et al, 2015 ). Previous reports demonstrated that Ca 2+ selectively enhanced MAO activity in mice, rats, monkeys, and human Brains.…”

Section: Discussionmentioning

confidence: 99%

Isoliquiritigenin Alleviates Semen Strychni-Induced Neurotoxicity by Restoring the Metabolic Pathway of Neurotransmitters in Rats

et al. 2021

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Acute neurotoxicity of Semen Strychni can result in sudden death in epilepsy. The detoxification method and mechanism of Semen Strychni acute poisoning have not been clarified. This experiment focused on the mechanism of Semen Strychni neurotoxicity and the alleviation effects of isoliquiritigenin. The rats were intraperitoneally injected with Semen Strychni extract (125 mg/kg), followed by oral administration of isoliquiritigenin (50 mg/kg) for 7 days. FJ-B staining was used to evaluate the degree of injury on hippocampus neurons. The concentration of monoamines, amino acids, and choline neurotransmitters, the Dopamine (DA) and 5-hydroxytryptamine (5-HT) metabolic pathway in the hippocampus, cerebellum, striatum, prefrontal cortex, hypothalamus, serum, and plasma were detected by LC-MS/MS. The expression of neurotransmitter metabolic enzymes [catechol-O-methyl transferase (COMT) and monoamine oxidase (MAO)] and neurotransmitter receptors [glutamate N-methyl-D-aspartic acid receptors (NMDARs) and gamma-aminobutyric acid type A receptor (GABRs)] were, respectively determined using ELISA and qRT-PCR. The results indicated that Semen Strychni induced neuronal degeneration in the hippocampal CA1 region. Meanwhile, Semen Strychni inhibited the mRNA expression of NMDAR1, NMDAR2A, NMDAR2B, GABRa1, GABRb2 and reduced the level of MAO, which disrupted the DA and 5-HT metabolic pathway. However, isoliquiritigenin reversed these effects. In summary, isoliquiritigenin showed alleviation effects on Semen Strychni-induced neurotoxicity, which could be attributed to restoring neurotransmitters metabolic pathway, most likely through the activation of NMDA receptors.

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Section: Discussionmentioning

confidence: 99%

Isoliquiritigenin Alleviates Semen Strychni-Induced Neurotoxicity by Restoring the Metabolic Pathway of Neurotransmitters in Rats

et al. 2021

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“…5-HT is one of the best-defined neurotransmitter systems in planarians with neurons, receptors, and metabolic pathways all being identified (Saitoh, 1996;Ribeiro et al, 2005, Welsh andWilliams, 1970). Levels of 5-HT have been quantified in planarians, with tissue concentrations being greater in head versus tail fragments (Wu et al, 2015;Buttarelli et al, 2008;Ness et al, 1996, Umeda et al, 2005. Also identified in planarians are a monoamine oxidase pathway that facilitates 5-HT catabolism and an ortholog gene of tryptophan hydroxylase (designated DjTPH), the rate-limiting enzyme for 5-HT biosynthesis (Wu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Levels of 5-HT have been quantified in planarians, with tissue concentrations being greater in head versus tail fragments (Wu et al, 2015;Buttarelli et al, 2008;Ness et al, 1996, Umeda et al, 2005. Also identified in planarians are a monoamine oxidase pathway that facilitates 5-HT catabolism and an ortholog gene of tryptophan hydroxylase (designated DjTPH), the rate-limiting enzyme for 5-HT biosynthesis (Wu et al, 2015). DjTPH mRNA and protein are mainly expressed in the planarian nervous system, and DjTPH immunoreactivity is present at axonal connections in the ventral nerve cords (Nishimura et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Predator odor produces anxiety-like behavioral phenotype in planarians that is counteracted by fluoxetine

Cho

Nayak

Jennings

et al. 2019

Physiology & Behavior

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Avoidant behavior is a characteristic feature post-traumatic stress disorder (PTSD) and is modeled in mammals with predator odor. Light avoidance is a hallmark behavioral reaction in planarians. We hypothesized that planarians exposed to frog extract would display enhanced light avoidance that is prevented by fluoxetine. Enhanced light avoidance (i.e., less time spent in light compartment of a dish split into light and dark sides) after a 30-min frog extract exposure (0.0001-0.01%) manifested 15 min post-exposure, persisted for at least 24 h, and was counteracted by fluoxetine (10 μM). These results suggest conservation of an anxiety-like behavioral phenotype.

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“…Cadmium (Cd) has been found to bind to microplastic in many environments (Almeida et al, 2020; Banaee et al, 2019; Godoy et al, 2019). Cadmium is a metal of interest because exposure to Cd causes adverse effects in many freshwater invertebrates and it is ubiquitous in freshwater environments, particularly in industrial zones (Almeida et al, 2003; Gill & Epple, 1992; Outridge et al, 1994; Salánki & Hiripi, 1990; Wu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of Exposure to Cadmium, Microplastics, and Their Mixture on Survival, Growth, Feeding, and Life History of Daphnia magna

Zink

Shearer

Wiseman

et al. 2023

Environmental Toxicology and Chemistry

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There is concern that microplastics can act as a vector for cadmium (Cd), altering the bioavailability and subsequent toxicity of Cd to ecologically important species such as Daphnia magna. The toxicity of Cd to D. magna has been well described; however, what is not known, and what the present study addresses, was how the addition of polyethylene microplastic altered Cd toxicity. Using high‐throughput feeding assays and size assessments, the present study quantified effects of exposure to Cd, microplastic, or their mixture on daphnids from neonate to adult. Exposure to Cd inhibited feeding efficiency, while exposure to microplastic inhibited growth rates of juveniles. Daphnia magna coexposed to Cd and microplastic showed significant decreases in both feeding and prereproductive growth rate. There were no differences in life‐history traits across any treatments. The alterations of feeding and growth while maintaining reproductive endpoints (time to first brood, reproductive frequency, the number of neonates released at each reproductive event, and the size of neonates produced) might be the result of a shift in energy allocation away from somatic growth, allowing individuals to maintain reproductive output despite lower nutritional reserves. Our findings suggest that cocontamination of microplastic and Cd has additive effects on feeding and growth rates, resulting in a greater energy allocation shift. Environ Toxicol Chem 2023;42:1401–1408. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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Disturbances to neurotransmitter levels and their metabolic enzyme activity in a freshwater planarian exposed to cadmium

Cited by 21 publications

References 44 publications

Isoliquiritigenin Alleviates Semen Strychni-Induced Neurotoxicity by Restoring the Metabolic Pathway of Neurotransmitters in Rats

Isoliquiritigenin Alleviates Semen Strychni-Induced Neurotoxicity by Restoring the Metabolic Pathway of Neurotransmitters in Rats

Predator odor produces anxiety-like behavioral phenotype in planarians that is counteracted by fluoxetine

Effects of Exposure to Cadmium, Microplastics, and Their Mixture on Survival, Growth, Feeding, and Life History of Daphnia magna

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