Disturbing-Free Determination of Yeast Concentration in DI Water and in Glucose Using Impedance Biochips

Kiani, Mahdi; Du, Nan; Vogel, Manja; Raff, Johannes; Hübner, Uwe; Skorupa, Ilona; Bürger, Danilo; Schulz, Stefan E.; Schmidt, Oliver G.; Blaschke, Daniel; Schmidt, Heidemarie

doi:10.3390/bios10010007

Cited by 6 publications

(7 citation statements)

References 24 publications

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“…We investigated single cells and a set of up to 10 9 cells/mL from a batch of L. sphaericus JG-A12 using standard methods (atomic force microscopy ( Section 2.1 ), optical microscopy and optical density measurements, and plating experiments ( Section 2.2 ) and by using the newly established impedance spectroscopy measurements on impedance biochips [ 16 , 17 , 18 ] ( Section 2.3 ). Two different equivalent circuit models have been developed to extract the effective impedance change Z(ω) of the impedance chips after addition of 1, 2, 3, 4 and 5 µL of liquid with live cells and 1, 2, 3, 4 and 5 µL of liquid with dead cells to 20 µL DI water.…”

Section: Resultsmentioning

confidence: 99%

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Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips

Bhat

Vegesna

Kiani

et al. 2021

IJMS

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Using two different types of impedance biochips (PS5 and BS5) with ring top electrodes, a distinct change of measured impedance has been detected after adding 1–5 µL (with dead or live Gram-positive Lysinibacillus sphaericus JG-A12 cells to 20 µL DI water inside the ring top electrode. We relate observed change of measured impedance to change of membrane potential of L. sphaericus JG-A12 cells. In contrast to impedance measurements, optical density (OD) measurements cannot be used to distinguish between dead and live cells. Dead L. sphaericus JG-A12 cells have been obtained by adding 0.02 mg/mL of the antibiotics tetracycline and 0.1 mg/mL chloramphenicol to a batch with OD0.5 and by incubation for 24 h, 30 °C, 120 rpm in the dark. For impedance measurements, we have used batches with a cell density of 25.5 × 108 cells/mL (OD8.5) and 270.0 × 108 cells/mL (OD90.0). The impedance biochip PS5 can be used to detect the more resistive and less capacitive live L. sphaericus JG-A12 cells. Also, the impedance biochip BS5 can be used to detect the less resistive and more capacitive dead L. sphaericus JG-A12 cells. An outlook on the application of the impedance biochips for high-throughput drug screening, e.g., against multi-drug-resistant Gram-positive bacteria, is given.

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Section: Resultsmentioning

confidence: 99%

“…The implantation parameters are given in Refs. [ 16 , 17 , 18 ]. Impedance has been recorded in the frequency range between 40 Hz and 1 MHz before filling (No fill) and after filling 20 µL DI water, and after subsequently adding 1, 2, 3, 4, and 5 µL liquid into the ring electrode area of the impedance biochips.…”

Section: Resultsmentioning

confidence: 99%

Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips

Bhat

Vegesna

Kiani

et al. 2021

IJMS

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“…Among these methods, DS is a non-invasive, fast, and promising method for cell screening. DS has been widely used for impedance measurements of yeast cells [10][11][12], breast cancer cells [13], lymphocyte activation profiling [14], monitoring performance of epithelial tissues [15], and hepatology research [16].…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Dielectric Spectroscopy System for Characterization of Biological Cells in Physiological Media

Bakhtiari

Manshadi

Mansoorifar

et al. 2022

Sensors

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Dielectric spectroscopy (DS) is a promising cell screening method that can be used for diagnostic and drug discovery purposes. The primary challenge of using DS in physiological buffers is the electrode polarization (EP) that overwhelms the impedance signal within a large frequency range. These effects further amplify with the miniaturization of the measurement electrodes. In this study, we present a microfluidic system and the associated equivalent circuit models for real-time measurements of cell membrane capacitance and cytoplasm resistance in physiological buffers with 10 s increments. The current device captures several hundreds of biological cells in individual microwells through gravitational settling and measures the system’s impedance using microelectrodes covered with dendritic gold nanostructures. Using PC-3 cells (a highly metastatic prostate cancer cell line) suspended in cell growth media (CGM), we demonstrate stable measurements of cell membrane capacitance and cytoplasm resistance in the device for over 15 min. We also describe a consistent application of the equivalent circuit model, starting from the reference measurements used to determine the system parameters. The circuit model is tested using devices with varying dimensions, and the obtained cell parameters between different devices are nearly identical. Further analyses of the impedance data have shown that accurate cell membrane capacitance and cytoplasm resistance can be extracted using a limited number of measurements in the 5 MHz to 10 MHz range. This will potentially reduce the timescale required for real-time DS measurements below 1 s. Overall, the new microfluidic device can be used for the dielectric characterization of biological cells in physiological buffers for various cell screening applications.

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“…Stilman and his colleagues [19] detected yeast strains in food samples such as yoghurt and beer by combining surface-imprinted polymers with impedance spectroscopy. Kiani et al [20] fabricated a biochip for the determination of yeast concentration in different media based on impedance measurements. Yao et al [21] studied the dielectric properties of yeast cells by impedance spectroscopy to detect the status and concentration of the cell suspension.…”

Section: Introductionmentioning

confidence: 99%

Modeling of an Impedimetric Biosensor with Ultrasonic-Assisted Cell Alignment for the Detection of Yeast

Tian

Xie

et al. 2022

Journal of Sensors

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Yeast is an integral part of our environment. The detection of yeast is of great significance in many fields. The impedance-based sensor with interdigital microelectrodes is a promising method to establish a simple and portable detection system. However, the positions of yeast cells greatly influence the impedance variation and eventually the sensitivity of detection. In this study, an impedimetric biosensor with ultrasonic-assisted cell alignment for yeast detection was proposed. In order to verify the feasibility of this biosensor, finite element modeling was conducted using the software COMSOL. The acoustic pressure field and the acoustic radiation force exerted on yeast cells were investigated. Cell positions in the microfluidic channel were determined using the fluid particle tracking module. After that, the impedance between the microelectrodes was calculated. Yeast suspensions with different cell concentrations were used as the tested samples for the simulation. The proposed sensor showed a higher sensitivity than the conventional impedimetric biosensor on which the cells were randomly located. It can be used for the detection of microorganisms. This finite element modeling provided an effective approach for the design of biosensors.

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Disturbing-Free Determination of Yeast Concentration in DI Water and in Glucose Using Impedance Biochips

Cited by 6 publications

References 24 publications

Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips

Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips

A Microfluidic Dielectric Spectroscopy System for Characterization of Biological Cells in Physiological Media

Modeling of an Impedimetric Biosensor with Ultrasonic-Assisted Cell Alignment for the Detection of Yeast

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