Ditylenchus sarvarae sp. n. (Tylenchina: Anguinidae) from Iran

Shokoohi, Ebrahim; Iranpour, F; Peneva, Vlada; Elshishka, Milka; Fourie, Hendrika; Swart, Antoinette

doi:10.11646/zootaxa.4399.2.4

Cited by 491 publications

(3 citation statements)

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“…Next, five µL of proteinase K and ten microliters of 5% Chelex® 50 were added to the microcentrifuge tubes that contained the crushed crayfish and mixed well. These tubes were incubated at 56 °C for two hours, then for 10 minutes at 95 °C to deactivate the proteinase K, and finally centrifuged for 2 min at 16000 x g (Sorvall™ Legend™ 14 Personal Microcentrifuge, USA) (Shokoohi et al, 2018). The supernatant was stored at -20 °C.…”

Section: Chelex Methodsmentioning

confidence: 99%

Comparison between two different DNA extraction methods to obtain high DNA quality from Astacus leptodactylus

Aminisarteshnizi¹

2022

Egypt. J. of Aquatic Biolo. and Fish.

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Section: Chelex Methodsmentioning

confidence: 99%

Comparison between two different DNA extraction methods to obtain high DNA quality from Astacus leptodactylus

Aminisarteshnizi¹

2022

Egypt. J. of Aquatic Biolo. and Fish.

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“…Thirty microliters of 5% Chelex® 50 and 2 µL of proteinase K were added to each of the microcentrifuge tubes that contained the crushed nematodes and mixed. These separate microcentrifuge tubes with the nematode lysate were incubated at 56°C for 2 and then incubated at 95°C for 10 min to deactivate the proteinase K and finally spin for 2 min at 16,000 rpm ( Shokoohi et al, 2018 ). The supernatant was then extracted from each of the tubes and stored at –20°C.…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular characters of Scutellonema brachyurus (Steiner, 1938) Andrássy, 1958 from South Africa

Shokoohi

2021

Journal of Nematology

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During a survey on plant-parasitic nematodes from South Africa, Scutellonema brachyurus was recovered from soil samples collected around the rhizosphere of wild grass in the North West and Limpopo provinces. This species characterized by a hemispherical lip region with four to six annuli, basal lip's annuli with longitudinal incisures, body length 696-904 µm (a = 25.1-33.5; b = 5.0-7.2; c = 48.9-75.3; c' = 0.5-0.9; V = 55-60), stylet 21-27 µm length, tail rounded with 10-19 µm length and spermatheca nonfunctional and male absent. The nblast analysis based on the D2-D3 segment of 28 S rDNA placed South African populations of S. brachyurus with 98% similarity to Greece (KU059494) and 99% similarity to South African (JX472052) S. brachyurus. Besides, nblast of COI of mtDNA showed 98% similarity of the test species with South African populations of S. brachyurus (JX472096; JX472097). The phylogenetic analysis put the South African populations of S. brachyurus together with other S. brachyurus with a 100 posterior probability support. Besides, the measurements, line illustration, and scanning electron microscopy photographs are provided for S. brachyurus from South Africa.

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“…After DNA amplification, 4 µL of product from each tube was loaded on a 1% agarose gel in TBE buffer (40 mM Tris, 40 mM boric acid and one mM EDTA) to evaluate the DNA bands. The PCR products were evaluated using Red Gel dye and visualized and photographed using a UV transilluminator 8 .…”

Section: Pcr Amplificationmentioning

confidence: 99%

Evaluation of Chelex 100 for DNA Extraction in Tomato Seedling (Solanum lycopersicum)

Aminisarte¹,

Mohlabe²,

Lediga³

2021

Asian J. of Plant Sciences

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Background and Objective: Tomato (Solanum lycopersicum) is a vegetable crop commonly used as a fresh vegetable or as a spice for food preparation. Extraction of genomic DNA with high quality for this critical vegetable is one of the basic needs of polymerase chain reaction. In modern research, PCR has found wide applications. Materials and Methods: This study had compared two different extraction DNA methods from tomato (S. lycopersicum). The DNA from the tomato was extracted using Chelex "method 1" (overnight incubation at 56EC) and "method 2" (Ten minutes incubation at 95EC) from the fresh leaves of the tomato. For this comparison, we used four samples of S. lycopersicum from South Africa during 2021. Quantitative and qualitative parameters were measured using a spectrophotometer. To confirm and evaluate the extracted DNA, the PCR reaction with primers for 28S was performed on all samples. Results: The results for the spectrophotometer showed that the highest quality of extracted DNA was in "method 2" (1.59-1.64). However, the protein (1.46-1.50 mg mLG 1 ) was detected in the tested samples through "method 1".The qualitative and quantitative tests for PCR reaction showed that the DNA extracted using "method 2" had better quality than "method 1". Amplification of samples with 28S primer showed higher concentration and purity of DNA extracted with "method 2". Conclusion: In conclusion, both methods worked, but method two showed better results regarding time and high-quality DNA.

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Ditylenchus sarvarae sp. n. (Tylenchina: Anguinidae) from Iran

Cited by 491 publications

References 0 publications

Comparison between two different DNA extraction methods to obtain high DNA quality from Astacus leptodactylus

Comparison between two different DNA extraction methods to obtain high DNA quality from Astacus leptodactylus

Morphological and molecular characters of Scutellonema brachyurus (Steiner, 1938) Andrássy, 1958 from South Africa

Evaluation of Chelex 100 for DNA Extraction in Tomato Seedling (Solanum lycopersicum)

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