DIURNAL CHANGES AND GROWTH RATES AS ASSOCIATED WITH ASCORBIC ACID, TITRATABLE ACIDITY, CARBOHYDRATE AND NITROGENOUS FRACTIONS IN THE LEAVES OF <i>ANANAS COMOSUS</i> (L.) MERR

Sideris, C. P.; Young, H. Y.; Chun, Hong

doi:10.1104/pp.23.1.38

Cited by 57 publications

(34 citation statements)

References 49 publications

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“…Many investigators have contributed toward the elucidation of this assumption but the final goal has not yet been reached. The literature on the subject has been ably surveyed by VICKERY et al (19), PUCHER et al (11), and SIDERIS et al (16) and will not be reviewed in detail in the present paper.…”

Section: Introductionmentioning

confidence: 99%

Respiration Studies on Chlorella. I. Growth Experiments With Acid Intermediates

Eny

1950

Plant Physiol.

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IntroductionThe study of carbohydrate metabolism has received considerable attention in the animal domain. It has been assumed by many that carbohydrate metabolism in higher plants follows a similar path. Many investigators have contributed toward the elucidation of this assumption but the final goal has not yet been reached. The literature on the subject has been ably surveyed by VICKERY et al. (19), PUCHER et al. (11), and SIDERIS et al. (16) and will not be reviewed in detail in the present paper.The reactions occurring during the metabolism of carbohydrates are separated into two main schemes-one taking place under anaerobic, the other under aerobic conditions. The problems having received the most attention are largely concerned with anaerobic processes such as the fermentation of sugar to ethyl alcohol and the formation of lactic acid. The reactions leading to the complete oxidation of carbohydrates under aerobic conditions (i.e. respiration) are not as well understood.The object of the present investigation is to study the possible steps involved in the respiratory mechanism of plant tissues inasmuch as they are connected with the aerobic path of carbohydrates, by determining the extent to which some of the metabolic intermediates known for animal tissues fit into the plant domain.The program of research was planned to include several parts, the first being the study of the growth of chlorella when supplied with organic acids known to enter in the path of aerobic carbohydrate metabolism in animal tissues. Other portions are concerned with the effects of organic acids on respiration and the study of the action of various enzymes and enzyme inhibitors. These will be reported in subsequent publications. Materials and methodsMATERIALS.-The strain of chlorella used was originally isolated from soil by WANN (20) and has been maintained in pure culture in the Plant Physiology laboratory at Cornell University. According to CLARK (2), the species has not been described taxonomically.The chlorella used were all derived from a single cell obtained by plating out a suspension of cells at the beginning of the investigation. The Stock culture vessels were inoculated with a transfer loop and the organic acid cultures were inoculated with pipettes. The pipettes used for inoculation were sterilized in a hot air oven at 160°C to two hours. A small wad of cotton was inserted in the mouth end before sterilization.The flasks were then placed in the insulated thermostated chamber as described by MANDELS (8) and were automatically shaken for five minutes every hour. The light intensity applied for stock cultures was about 500 foot candles. On the other hand, the organic acid cultures were kept in the dark. For this purpose, the insulated chamber was protected from light by four layers of black cotton cloth. In both cases, the temperature of the chamber was adjusted to 280 C ± .5°.In preparing the inoculum for organic acid cultures, a known amount of cells was needed to permit an accurate measurement of growth. To free the...

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Section: Introductionmentioning

confidence: 99%

Respiration Studies on Chlorella. I. Growth Experiments With Acid Intermediates

Eny

1950

Plant Physiol.

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“…Also, sugar concentrations, in inverse relation to organic acids, varied between chlorophyllous and non-chlorophyllous tissues of the leaves. However, differences in sugar or organic acid concentrations between comparable tissues of leaves of different plants were related to differences in the rates of growth of said plants (12). Sugar concentrations for culture A, in figure 5 A, were higher in the basal than in all other sections of the active D leaves at all growth intervals, except in September 1942 and May 1943, in the early and late stages of growth.…”

Section: Plant Physiologymentioning

confidence: 98%

“…Experimental results of the authors (12) indicated that the leaves of pineapple plants accumulated at night, in darkness, great quantities of di-or tri-carboxylic acids, i.e., malic or citric, which disappeared during the day, in light. The rates of organic acid accumulation increased with higher growth rates and vice versa.…”

Section: Plant Physiologymentioning

confidence: 99%

“…For instance, malic and citric acid concentrations, at night or under reduced.light conditions, were greater in the chlorophyllous than non-chlorophyllous tissues of the same leaves (10,11,12), due to differences in the metabolic activities of same. Also, sugar concentrations, in inverse relation to organic acids, varied between chlorophyllous and non-chlorophyllous tissues of the leaves.…”

Section: Plant Physiologymentioning

confidence: 99%

“…SUGARS Previous results (8,10,12) revealed that sugar concentrations in the leaves were intimately associated with the metabolic activities of the tissues of different sections. For example, sugar concentrations in chlorophyllous distal sections were generally related to the rates of photosynthesis and of translocation to other parts of the plant, while in non-chlorophyllous basal sections they were related to the rates of translocation from the chlorophyllous sections and of utilizations by meristematic tissues in growth or of polymerization in specialized storage tissues.…”

Section: Plant Physiologymentioning

confidence: 99%

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GROWTH OF ANANAS COMOSUS (L.) MERR., AT DIFFERENT LEVELS OF MINERAL NUTRITION UNDER GREENHOUSE AND FIELD CONDITIONS. II. CHEMICAL COMPOSITION OF THE TISSUES AT DIFFERENT GROWTH INTERVALS

Sideris¹,

Young²

1951

Plant Physiol.

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This report supplements the earlier report (13) which discussed the growth rates, water and mineral nutrient intake by the same group of plants. The data herein considered deal with the chemical composition of the leaf and stem tissues at different growth intervals of pineapple plants grown in nutrient solutions in the greenhouse and in soil under field conditions with different amounts of nitrate, potassium, and calcium.The plant tissues were analyzed for total nitrogen, soluble organic nitrogen, potassium, calcium, reducing and total sugars; the latter hydrolyzed by invertase. The methods for potassium and calcium were reported in a previous publication (9). Total nitrogen and soluble organic nitrogen were determined by the Kjeldahl procedure, using selenium oxychloride as catalyst in the oxidation of organic matter. The fractionation of total and soluble organic nitrogen was conducted according to technique reported previously (7,8). Reducing sugars, total sugars, and sucrose, the latter by the difference between total and reducing sugars after hydrolysis with invertase, were determined according to the method of QUISUMBING and THOMAS (6). Starch was determined by the method of PUCHER and VICKERY (5). Results GREENHOUSE PLANTSThe chemical composition of the nutrient solutions in the different cultures, reported previously (13)

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Effects of Chlorides on the Metabolism of Pineapple Plants

Sideris¹,

Young²

1954

American J of Botany

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DIURNAL CHANGES AND GROWTH RATES AS ASSOCIATED WITH ASCORBIC ACID, TITRATABLE ACIDITY, CARBOHYDRATE AND NITROGENOUS FRACTIONS IN THE LEAVES OF ANANAS COMOSUS (L.) MERR

Cited by 57 publications

References 49 publications

Respiration Studies on Chlorella. I. Growth Experiments With Acid Intermediates

Respiration Studies on Chlorella. I. Growth Experiments With Acid Intermediates

GROWTH OF ANANAS COMOSUS (L.) MERR., AT DIFFERENT LEVELS OF MINERAL NUTRITION UNDER GREENHOUSE AND FIELD CONDITIONS. II. CHEMICAL COMPOSITION OF THE TISSUES AT DIFFERENT GROWTH INTERVALS

Effects of Chlorides on the Metabolism of Pineapple Plants

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