Diurnal Changes in Photosynthesis in Sugarcane Leaves: II. Enzyme activities and metabolite levels relating to sucrose and starch metabolism

Du, Yong; Nose, Akihiro; Kondo, Akimasa; Wasano, Kikuo

doi:10.1626/pps.3.9

Cited by 21 publications

(10 citation statements)

References 25 publications

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“…The application of a cold-girdle also had similar consequences for photosynthesis as the accumulation of sugar through the course of a day. This accumulation of sucrose in leaves of field-grown sugarcane during the day represents an imbalance between the rate of photosynthesis and the rate of export, which is in turn dependent on carbon demand from culm tissue [15,47]. In the current study, neither the photosynthetic rate nor the "end of day" leaf sucrose concentration varied significantly over time in the control plants, indicating that supply and demand were dynamically matched, as also observed in other C 4 species [33].…”

Section: Discussionsupporting

confidence: 39%

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

McCormick

Cramer

Watt

2008

Tropical Plant Biol.

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In C 4 sugarcane (Saccharum spp. hybrids), photosynthetic activity has been shown to be regulated by the demand for carbon from sink tissues. There is evidence, from other plant species, that sink-limitation of photosynthesis is facilitated by sugar-signaling mechanisms in the leaf that affect photosynthesis through regulation of gene expression. In this work, we manipulated leaf sugar levels by cold-girdling leaves (5°C) for 80 h to examine the mechanisms whereby leaf sugar accumulation affects photosynthetic activity and assess whether signaling mechanisms reported for other species operate in sugarcane. During this time, sucrose and hexose concentrations above the girdle increased by 77% and 81%, respectively. Conversely, leaf photosynthetic activity (A) and electron transport rates (ETR) decreased by 66% and 54%, respectively. Quantitative expression profiling by means of an Affymetrix GeneChip Sugarcane Genome Array was used to identify genes responsive to cold-girdling (56 h). A number of genes (74) involved in primary and secondary metabolic pathways were identified as being differentially expressed. Decreased expression of genes related to photosynthesis and increased expression of genes involved in assimilate partitioning, cell wall synthesis, phosphate metabolism and stress were observed. Furthermore four probe sets homologous to trehalose 6-phosphate phosphatase (TPP; EC 5.3.1.1) and trehalose 6-phosphate synthase (TPS; EC 2.4.1.15) were up-and down-regulated, respectively, indicating a possible role for trehalose 6-phosphate (T6P) as a putative sugar-sensor in sugarcane leaves.

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Section: Discussionsupporting

confidence: 39%

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

McCormick

Cramer

Watt

2008

Tropical Plant Biol.

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“…Sugarcane leaves have been shown to primarily accumulate and also export sucrose during the photosynthetic period (Hartt and Kortchak, 1960;Lunn and Hatch, 1995;Du et al, 2000). Sucrose is readily exported from sugarcane leaves, with up to 80% of assimilate reportedly being exported immediately at midday (Du et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Regulation of photosynthesis by sugars in sugarcane leaves

McCormick

Cramer

Watt

2008

Journal of Plant Physiology

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“…The translocation of sugars in sugarcane occurs through the leaf blade and leaf sheath to the stalk, via the phloem, then to the centre of the stalk and downward to roots (Alexander, 1973). During the day, sugars are temporarily accumulated in leaf sheaths before their translocation to the stalk, with about 80% of the carbon fixed during early morning hours being exported after midday (Du et al., 2000). Phloem functionality is therefore a requisite for appropriated sugar movement within the plant (Bieleski, 2000), and the phloem vessels are the main target of the ScYLV infection.…”

Section: Discussionmentioning

confidence: 99%

Physiological Performance of Asymptomatic and Yellow Leaf Syndrome‐affected Sugarcanes in Venezuela

Izaguirre‐Mayoral

Carballo

Alceste

et al. 2002

Journal of Phytopathology

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Serological analyses revealed the presence of the sugarcane yellow leaf virus (ScYLV) in asymptomatic (S–) and symptomatic (S+) yellow leaf syndrome‐affected sugarcane plants of the cultivars PR.692176, C.323–68, V.64–10, V.71–47, V.75–6, SP.72–2086, SP.72–1210, SP.74–2005, C.323–68, B.80–549 and B.82–363. Tests for the presence of the sugarcane yellows phytoplasma, carried out by Dr P. Jones (IACR‐Rothamsted), gave negative results in all cultivars. Physiological analyses were performed in the top visible dewlap (TVD) leaf of S– and S+ plants of the cultivar PR.692176. All plants were at the second ratoon and flowering. When compared with S– plants, the S+ plants showed: (a) a marked reduction in the area of the leaf and internodes; (b) a high accumulation of total reducing sugars (TRS), glucans and α‐amino‐N in the leaf blade and of TRS in the corresponding leaf sheath; (c) a decrease in the chlorophyll, phosphorus and nitrogen content in the leaf; (d) the disappearance of the leaf diurnal fluctuations in TRS accumulation and export as well as the daily oscillations of TRS and glucans between dawn and dusk; and (e) major ultrastructural alterations in the companion cells of the phloem, including the accumulation of ScYLV particles in the cytoplasm. In S– plants, none of the growth and physiological alterations described above were observed, in spite of the high density of ScYLV particles in the cytoplasm. The location of S– and S+ plants close to each other without a discernible pattern of distribution in plots subjected to optimal irrigation and fertilization rule out the possibility that environmental conditions underlay the appearance of symptoms. In plots under severe drought for 3 months, however, all S– plants become S+. Symptom expression did not affect the acid phosphatase activity in the rhizosphere of S+ plants.

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Diurnal Changes in Photosynthesis in Sugarcane Leaves: II. Enzyme activities and metabolite levels relating to sucrose and starch metabolism

Cited by 21 publications

References 25 publications

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

Differential Expression of Genes in the Leaves of Sugarcane in Response to Sugar Accumulation

Regulation of photosynthesis by sugars in sugarcane leaves

Physiological Performance of Asymptomatic and Yellow Leaf Syndrome‐affected Sugarcanes in Venezuela

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