Diurnal Periodicity of the Expression of Genes Involved in Monolignol Biosynthesis in Differentiating Xylem of &amp;lt;i&amp;gt;Cryptomeria japonica&amp;lt;/i&amp;gt;

Sato, Saori; Yoshida, Masato; Ashizaki, Yousuke; Yamamoto, Hiroyuki

doi:10.4236/ajps.2016.717214

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Given that the processes governing xylem cell expansion, cellulose and hemicellulose synthesis display diurnal plasticity, primarily occurring predawn/night ( Solomon et al. 2010 ; Sato et al. 2016 ; Buttò et al.…”

Section: Introductionmentioning

confidence: 99%

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Keret,

Drew,

Hills

2024

Tree Physiology

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Future climatic scenarios forecast increasingly frequent droughts that will pose substantial consequences on tree mortality. In light of this, drought-tolerant eucalypts have been propagated, however, the severity of these conditions will invoke adaptive responses, impacting the commercially valuable wood properties. To determine what mechanisms govern the wood anatomical adaptive response, highly controlled drought experiments were conducted in E. grandis, with the tree physiology and transcriptome closely monitored. In response to water deficit, E. grandis displays an isohydric stomatal response to conserve water and enable stem growth to continue, albeit at a reduced rate. Maintaining gaseous exchange is likely a critical short-term response that drives the formation of hydraulically-safer xylem. For instance, the development of significantly smaller fibers and vessels was found to increase cellular density, thereby promoting drought tolerance through improved functional redundancy, as well as implosion and cavitation resistance. The transcriptome was explored to identify the molecular mechanisms responsible for controlling xylem cell size during prolonged water deficit. Downregulation of genes associated with cell wall remodelling and the biosynthesis of cellulose, hemicellulose, and pectin appeared to coincide with a reduction in cellular enlargement during drought. Furthermore, transcript levels of NAC and MYB transcription factors, vital for cell wall component biosynthesis, were reduced, while those linked to lignification increased. The upregulation of EgCAD and various peroxidases under water deficit did not correlate with an increased lignin composition. However, with the elevated cellular density, a higher lignin content per xylem cross-sectional area was observed, potentially enhancing hydraulic safety. These results support the requirement for higher density, drought-adapted wood as a long-term adaptive response in E. grandis, which is largely influenced by the isohydric stomatal response coupled with cellular expansion-related molecular processes.

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Section: Introductionmentioning

confidence: 99%

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Keret,

Drew,

Hills

2024

Tree Physiology

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“…Analogously, the growing season of Japanese cedar, another gymnosperm, runs from March until October, with lignin synthesis activity sharply increasing from March to June, then declining until dormancy in October [31]. Interestingly, Sato et al [35] analyzed the diurnal periodicity of expression of lignin synthesizing genes in C. japonica and found that most enzymes showed different expression abundance at different times on the same day. This paper provides a good research direction for our future study.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling of Cryptomeria fortunei Hooibrenk Vascular Cambium Identifies Candidate Genes Involved in Phenylpropanoid Metabolism

Yang

Guo

Zhang

et al. 2020

Forests

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Cryptomeria fortunei Hooibrenk (Chinese cedar) is a coniferous tree from southern China that has an important function in landscaping and timber production. Lignin is one of the key components of secondary cell walls, which have a crucial role in conducting water and providing mechanical support for the upward growth of plants. It is mainly biosynthesized via the phenylpropanoid metabolic pathway, of which the molecular mechanism remains so far unresolved in C. fortunei. In order to obtain further insight into this pathway, we performed transcriptome sequencing of the C. fortunei cambial zone at 5 successive growth stages. We generated 78,673 unigenes from transcriptome data, of which 45,214 (57.47%) were successfully annotated in the non-redundant protein database (NR). A total of 8975 unigenes were identified to be significantly differentially expressed between Sample_B and Sample_A after analyzing their expression profiles. Of the differentially expressed genes (DEGs), 6817 (75.96%) and 2158 (24.04%) were up- and down-regulated, respectively. 83 DEGs were involved in phenylpropanoid metabolism, 37 DEGs that encoded v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factor (TF), and many candidates that encoded lignin synthesizing enzymes. These findings contribute to understanding the expression pattern of C. fortunei cambial zone transcriptome. Furthermore, our results provide additional insight towards understanding the molecular mechanisms of wood formation in C. fortunei.

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Diurnal Periodicity of the Expression of Genes Involved in Monolignol Biosynthesis in Differentiating Xylem of <i>Cryptomeria japonica</i>

Cited by 2 publications

References 25 publications

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Transcriptomic Profiling of Cryptomeria fortunei Hooibrenk Vascular Cambium Identifies Candidate Genes Involved in Phenylpropanoid Metabolism

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