Diurnal Rhythm of β-Carotene in Photosynthetic Alga<i>Gonyaulax polyedra</i>

Mascio, Paolo Di; Hollnagel, Heloisa Candia; Sperança, M; Colepicolo, Pio

doi:10.1515/bchm3.1995.376.5.297

Cited by 17 publications

(9 citation statements)

References 21 publications

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“…In Gonyaulax, their concentration varies over the circadian day, and de-novo synthesis can be induced by light stimulation during the dark phase (with a maximum around midnight; Di Mascio et al 1995). However, when we inhibited their synthesis by Norurazon, we observed no speci®c eects on circadian parameters such as changes in period, phase, or amplitude.…”

Section: Resultsmentioning

confidence: 63%

Photobiology of the Gonyaulax circadian system. II. Allopurinol inhibits blue-light effects

Deng

Roenneberg

1997

Planta

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Light is the main environmental signal (zeitgeber) for practically all circadian systems, but little is known about the transduction mechanisms by which light signals reach the circadian oscillator. To identify components involved in the circadian light transduction pathway in the unicellular alga Gonyaulax polyedra Stein, we assayed inhibitors of pigment synthesis and of¯avo-enzymes for their eects on circadian properties such as phase and period. We found that allopurinol, an inhibitor of xanthine oxidoreductase, speci®cally inhibits the period and phase eects mediated by the blue-light-sensitive input pathway, while the other light input of the Gonyaulax circadian system, that is sensitive to both red and blue light, appears to be unaected.

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Section: Resultsmentioning

confidence: 63%

Photobiology of the Gonyaulax circadian system. II. Allopurinol inhibits blue-light effects

Deng

Roenneberg

1997

Planta

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“…[14][15][16][22][23][24] The C 30 stationary phase has been widely used for carotenoid separations since, in general, it provides significant resolution of complex mixtures. 22,23 In addition to the column selection, mobile phase choice is extremely important.…”

Section: Resultsmentioning

confidence: 99%

“…Many methods for the separation of pigments from algae have been developed. 14,15 However, the complexity of their composition makes the resolution of all pigments from different algae species difficult in a single run method.…”

Section: Introductionmentioning

confidence: 99%

Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids

Guaratini¹,

Cardozo²,

Pinto³

et al. 2009

J. Braz. Chem. Soc.

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A determinação qualitativa e quantitativa de carotenóides pode fornecer diferentes e importantes informações sobre os organismos que os contêm. Na análise de pigmentos por HPLC diversos detectores podem ser utilizados, como diode array (DAD) e eletroquímico (ED). O presente trabalho tem como objetivo desenvolver um método por HPLC utilizando uma coluna C 30 para a identificação e quantificação de dezesseis pigmentos em diferentes classes de algas, além de comparar as respostas obtidas nos detectores DAD e ED por meio da análise dos resultados de precisão e exatidão. Apesar do ED ser geralmente um detector mais sensível que o DAD, os resultados de precisão e exatidão foram mais satisfatórios para o DAD. O método desenvolvido foi eficiente para a análise quantitativa dos pigmentos de cianobactérias e diferentes classes de algas, sendo que o padrão cromatográfico encontrado em cada classe foi discutido.Qualitative and quantitative determination of carotenoids pigments can provide valuable information about the organisms in which this important class of compounds is found. In the HPLC analysis of pigments, diode array (DAD), electrochemical (ED) and other kinds of detector may be used. The aim of this work is to develop an HPLC method using a C 30 column to identify and quantify sixteen different pigments from algae. A further aim is to compare precision and accuracy obtained by DAD and ED. ED is normally more sensible than DAD. On the other hand, the highest precision and accuracy was obtained with DAD. In conclusion, the method was efficient for quantitative and qualitative analyses of pigments from cyanobacteria and different microalgae classes. Their pigment patterns for several organisms are also discussed.

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“…The photosynthetic apparatus in algae and higher plants provides the reducing power needed for the nitrate assimilation process and it is under light control (Lillo, 1994;Ninnemann, 1995;Di Mascio, 1995). The circadian rhythm in NR activity in Gracilaria somehow is regulated at the molecular level.…”

Section: Discussionmentioning

confidence: 99%

Effect of Light Quality on the Circadian Expression of Nitrate Reductase in the Red Macroalga Gracilaria tenuistipitata

Lopes¹,

Santa-Maria²,

Colepicolo³

2002

Biological Rhythm Research

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A circadian rhythm in the activity of nitrate reductase (NR: EC 1.6.6.1) isolated from the marine red algae Gracilaria tenuistipitata is shown to be attributable to the daily oscillation of protein levels. The experiments reported here indicate that light quality has differential effects over NR expression. In extracts of algae grown under white light : dark, red light : dark and blue light : dark cycle, the activity of NR peaks during photophase, as does photosynthesis. Staining with a monoclonal antibody (NR10), raised against NR purified from Porphyra yezoensis, shows that the amount of protein changes by a factor of about 20, with a maximum occurring during photophase when algae are submitted to white and blue light. Red light changes the circadian rhythm of NR protein levels and also inhibits its night degradation. Illumination with blue light is able to restore the NR activity as well as its protein levels only when the light irradiance was the same of the white light. Surprisingly, the red light promoted 40% induction on NR activity under the same conditions.

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Diurnal Rhythm of β-Carotene in Photosynthetic AlgaGonyaulax polyedra

Cited by 17 publications

References 21 publications

Photobiology of the Gonyaulax circadian system. II. Allopurinol inhibits blue-light effects

Photobiology of the Gonyaulax circadian system. II. Allopurinol inhibits blue-light effects

Comparison of diode array and electrochemical detection in the C30 reverse phase HPLC analysis of algae carotenoids

Effect of Light Quality on the Circadian Expression of Nitrate Reductase in the Red Macroalga Gracilaria tenuistipitata

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