Diurnal Variation in Mouse and Rat Liver Sulfhydryl.

Beck, Lyle V.; Rieck, Virginia D.; Duncan, Blendina

doi:10.3181/00379727-97-23698

Cited by 59 publications

(16 citation statements)

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“…The latter was withdrawn in the morning before an experiment and withheld throughout blood sampling. All experiments began between 08.30 and 09.30 h to minimize the pos sible influences of diurnal variations in serum sulfate [17], hepatic glutathione [18], and the activities of hepatic drug-metabolizing enzymes including the sulfotransferases [19][20][21].…”

Section: Methodsmentioning

confidence: 99%

Influence of Advanced Age on the Formation and Elimination of Acetaminophen Metabolites by Male Rats

Galinsky¹,

Corcoran²

1986

Pharmacology

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The effects of aging on acetaminophen metabolism and elimination in male Fischer 344 rats were examined after intravenous injection of 300 mg/kg. Age as a variable had only a small effect on the total clearance of acetaminophen. However, the fraction of administered dose recovered from urine as acetaminophen sulfate and the partial clearance to acetaminophen sulfate decreased while the fraction recovered as acetaminophen glucuronide and the partial clearance to acetaminophen glucuronide increased with increasing age. Renal clearances of acetaminophen and acetaminophen glucuronide were unchanged while that of acetaminophen sulfate decreased. These data point to an age-related decrease in sulfation and increase in glucuronidation of acetaminophen and further emphasize that the major conjugated metabolites are excreted by renal transport processes that operate under separate control. Moreover, they raise the possibility that advancing age may be accompanied by a general decline in processes that govern sulfate conjugate formation and elimination.

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Section: Methodsmentioning

confidence: 99%

Influence of Advanced Age on the Formation and Elimination of Acetaminophen Metabolites by Male Rats

Galinsky¹,

Corcoran²

1986

Pharmacology

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“…Some mechanism studies have focused on rhythmic variations in drug disposition in cluding metabolism and excretion [Radzialowski and Bousquet, 1968;Jon et al" 1971; Lake el al., 1976], on changes in the sensitiv ity o f the target issue to a particular agent [Roberts et al, 1970;Nelson and Halberg, 1973;Bornschein et al, 1973;Wolfe et al, 1979;Deimling and Schnell, 1980], and on rhythms in the physiological levels o f endog enous substances such as glutathione [Beck et al, 1958;Jaeger et al, 1973;Hanson and Anders, 1978;Harris and Anders. 1980;Schnell et al, 1983], We have previously reported studies which described circadian rhythms in the toxicity of acetaminophen in male mice.…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing Circadian Rhythms in Acetaminophen Lethality

et al. 1984

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Experiments were conducted to examine the effects of changes in lighting schedules and food consumption on circadian rhythms in acetaminophen lethality and hepatic glutathione levels in male mice. Under a normal lighting schedule (light: 06.00–18.00 h), male mice exhibited a circadian rhythm in acetaminophen lethality (peak: 18.00 h; nadir: 06.00, 10.00 h) and an inverse rhythm in hepatic glutathione concentrations (peak: 06.00, 10.00 h; nadir: 18.00 h). Under a reversed lighting schedule (light: 18.00–06.00 h) the glutathione rhythm was reversed and the rhythm in acetaminophen lethality was altered showing greater sensitivity to the drug. Under continuous light, there was a shift in the acetaminophen lethality and the hepatic glutathione rhythms. Under continuous dark, both rhythms were abolished. Under a normal lighting regimen, hepatic glutathione levels were closely correlated with food consumption; i.e., both were increased during the dark phase and decreased during the light phase. Fasting the mice for 12 h abolished the rhythms in acetaminophen lethality and hepatic glutathione levels; moreover, the lethality was increased and the hepatic glutathione levels were decreased. These experiments show that both lighting schedules and feeding can alter the circadian rhythms in acetaminophen lethality and hepatic glutathione levels in male mice.

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“…Work by Beck, Rieck and Duncan (1958) and Calcutt (1964Calcutt ( , 1967 has shown that well-defined diurnal variations occur in intracellular thiol levels, whilst Calcutt and Ting (1969) have found similar diurnal fluctuations in levels of disulphides in rat liver, spleen and kidney. The present results are therefore based on measurements made at a standard time of day, usually between 10 and 11 a.m. so as to avoid any diurnal variations.…”

Section: Resultsmentioning

confidence: 97%

The Acid Soluble Disulphide and Mixed Disulphide Levels of Some Normal Tissues and Transplanted Tumours

Calcutt¹,

Ting²

1970

Br J Cancer

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SUMMARY.-The acid soluble disulphides and mixed disulphides of a range of normal rat and mouse tissues and a number of transplanted rat or mouse tumours were measured. The results were considered in relation to other workers' data. It is noted that the more radioresponsive tissues have higher levels than the more radioresistant tissues.FOR a long time it was generally considered that the level of acid soluble disulphides in tumours was very low. Tarnowski, Barclay, Mountain, Nakamura, Satterwhite and Solney (1965) used a newly described reduction system-by means of sodium borohydride-and concluded that the concentration of acid soluble disulphides was comparable with the concentration of acid soluble thiols. This conclusion, however, was not warranted since Tarnowski et al. carried out their reductions on whole homogenates of tumours and then after deproteinisation estimated the thiol level for a comparison with that derived from a similar system without the reducing step. They therefore measured acid soluble disulphides plus any small acid soluble thiol groups broken off from proteins during the reduction process; that is the usually described " mixed disulphides ".Based on data derived from experiments with the Ehrlich ascites tumour Revesz (1969) has concluded that the acid soluble disulphides exist at a low level as compared with the mixed disulphides.To clarify this situation we have undertaken a new series of measurements of both acid soluble and mixed disulphides in a number of transplanted mouse and rat tumours. For comparison similar measurements have been made on a variety of normal mouse and rat tissues. METHODS Acid soluble disulphidesHaving considerable experience of the measurement of sulphydryl (-SH) groups by the technique described by Calcutt and Doxey (1959) andCalcutt, Doxey andCoates (1960) the system of measuring the difference between acid soluble thiols with and without reduction was preferred. Test runs involving a variety of reducing systems gave unsatisfactory results. The only method found to be repeatable and reliable was to homogenise a weighed amount of tissue in the presence of EDTA and o dipyridyl as described by Calcutt and Doxey (1962) and then to precipitate proteins with trichloracetic acid. After removal of the protein the acid solution

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Diurnal Variation in Mouse and Rat Liver Sulfhydryl.

Cited by 59 publications

References 0 publications

Influence of Advanced Age on the Formation and Elimination of Acetaminophen Metabolites by Male Rats

Influence of Advanced Age on the Formation and Elimination of Acetaminophen Metabolites by Male Rats

Factors Influencing Circadian Rhythms in Acetaminophen Lethality

The Acid Soluble Disulphide and Mixed Disulphide Levels of Some Normal Tissues and Transplanted Tumours

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