Diurnal variations in expression of photosynthesis-related proteins in the harmful Raphidophyceae Chattonella marina var. antiqua

Qiu, Xuchun; Mukai, Koki; Shimasaki, Yohei; Wu, Min; Chen, Chen; Lu, Yijiang; Ichinose, Hirofumi; Nakashima, Takashi; Kato-Unoki, Yoko; Oshima, Yuji

doi:10.1016/j.jembe.2020.151361

Cited by 6 publications

(5 citation statements)

References 47 publications

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“…We also saw a change in glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) expression, which was upregulated in red light compared to green. GAPDH catalyses the sixth step of glycolysis, though it can also be involved in mRNA regulation, tRNA export, and DNA replication or repair (Huang et al, 1989; Qiu et al, 2020; Sirover, 1998). This shift in GAPDH expression may be indicative of a shift in carbon metabolism or trophic strategy, such as decreasing photosynthetic function in favour of heterotrophic function (i.e., performing mixotrophy).…”

Section: Discussionmentioning

confidence: 99%

Consequences of light spectra for pigment composition and gene expression in the cryptophyte Rhodomonas salina

Schomaker,

Richardson,

Dudycha

2023

Environmental Microbiology

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Algae with a more diverse suite of pigments can, in principle, exploit a broader swath of the light spectrum through chromatic acclimation, the ability to maximize light capture via plasticity of pigment composition. We grew Rhodomonas salina in wide‐spectrum, red, green, and blue environments and measured how pigment composition differed. We also measured expression of key light‐capture and photosynthesis‐related genes and performed a transcriptome‐wide expression analysis. We observed the highest concentration of phycoerythrin in green light, consistent with chromatic acclimation. Other pigments showed trends inconsistent with chromatic acclimation, possibly due to feedback loops among pigments or high‐energy light acclimation. Expression of some photosynthesis‐related genes was sensitive to spectrum, although expression of most was not. The phycoerythrin α‐subunit was expressed two‐orders of magnitude greater than the β‐subunit even though the peptides are needed in an equimolar ratio. Expression of genes related to chlorophyll‐binding and phycoerythrin concentration were correlated, indicating a potential synthesis relationship. Pigment concentrations and expression of related genes were generally uncorrelated, implying post‐transcriptional regulation of pigments. Overall, most differentially expressed genes were not related to photosynthesis; thus, examining associations between light spectrum and other organismal functions, including sexual reproduction and glycolysis, may be important.

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Section: Discussionmentioning

confidence: 99%

Consequences of light spectra for pigment composition and gene expression in the cryptophyte Rhodomonas salina

Schomaker,

Richardson,

Dudycha

2023

Environmental Microbiology

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“…GAPDH catalyzes the sixth step of glycolysis, though it can also be involved in mRNA regulation, tRNA export, and DNA replication or repair (Huang et al . 1989; Sirover 1998; Qiu et al . 2020).…”

Section: Discussionmentioning

confidence: 99%

“…We also saw a change in glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression, which was upregulated in red light compared to green. GAPDH catalyzes the sixth step of glycolysis, though it can also be involved in mRNA regulation, tRNA export, and DNA replication or repair (Huang et al 1989;Sirover 1998;Qiu et al 2020). This shift in GAPDH expression may be indicative of a shift in carbon metabolism or trophic strategy, such as decreasing photosynthetic function in favor of heterotrophic function (i.e., performing mixotrophy).…”

Section: What Other Functions Respond To Light Spectrum At the Transc...mentioning

confidence: 99%

Consequences of light spectra for pigment composition and gene expression in the cryptophyteRhodomonas salina

Schomaker,

Richardson,

Dudycha

2023

Preprint

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Algae with a more diverse suite of pigments can, in principle, exploit a broader swath of the light spectrum through chromatic acclimation, the ability to maximize light capture via plasticity of pigment composition. We grew Rhodomonas salina in wide-spectrum, red, green, and blue environments and measured how pigment composition differed. We also measured expression of key light-capture and photosynthesis-related genes and performed a transcriptome-wide expression analysis. We observed the highest concentration of phycoerythrin in green light, consistent with chromatic acclimation. Other pigments showed trends inconsistent with chromatic acclimation, possibly due to feedback loops among pigments or high-energy light acclimation. Expression of some photosynthesis-related genes was sensitive to spectrum, although expression of most was not. The phycoerythrin α-subunit was expressed two-orders of magnitude greater than the β-subunit even though the peptides are needed in an equimolar ratio. Expression of genes related to chlorophyll-binding and phycoerythrin concentration were correlated, indicating a potential synthesis relationship. Pigment concentrations and expression of related genes were generally uncorrelated, implying post-transcriptional regulation of pigments. Overall, most differentially expressed genes were not related to photosynthesis; thus, examining associations between light spectrum and other organismal functions, including sexual reproduction and glycolysis, may be important.

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“…This rhythm continued after the cells were transferred to 24 h of darkness. In contrast, proteomic analysis revealed no clear circadian rhythm in the level of the 2-CysPrx protein [ 98 ]. Translation control as well as transcriptional control may, therefore, be important for the chloroplast-encoded 2-CysPrx gene of C. marina .…”

Section: Ecological Significance Of Circadian Rhythms In Hab Specimentioning

confidence: 99%

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

et al. 2021

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Peroxiredoxin (Prx) is a relatively recently discovered antioxidant enzyme family that scavenges peroxides and is known to be present in organisms from biological taxa ranging from bacteria to multicellular eukaryotes, including photosynthetic organisms. Although there have been many studies of the Prx family in higher plants, green algae, and cyanobacteria, few studies have concerned raphidophytes and dinoflagellates, which are among the eukaryotic algae that cause harmful algal blooms (HABs). In our proteomic study using 2-D electrophoresis, we found a highly expressed 2-Cys peroxiredoxin (2-CysPrx) in the raphidophyte Chattonella marina var. antiqua, a species that induces mass mortality of aquacultured fish. The abundance of the C. marina 2-CysPrx enzyme was highest in the exponential growth phase, during which photosynthetic activity was high, and it then decreased by about a factor of two during the late stationary growth phase. This pattern suggested that 2-CysPrx is a key enzyme involved in the maintenance of high photosynthesis activity. In addition, the fact that the depression of photosynthesis by excessively high irradiance was more severe in the 2-CysPrx low-expression strain (wild type) than in the normal-expression strain (wild type) of C. marina suggested that 2-CysPrx played a critical role in protecting the cell from oxidative stress caused by exposure to excessively high irradiance. In the field of HAB research, estimates of growth potential have been desired to predict the population dynamics of HABs for mitigating damage to fisheries. Therefore, omics approaches have recently begun to be applied to elucidate the physiology of the growth of HAB species. In this review, we describe the progress we have made using a molecular physiological approach to identify the roles of 2-CysPrx and other antioxidant enzymes in mitigating environmental stress associated with strong light and high temperatures and resultant oxidative stress. We also describe results of a survey of expressed Prx genes and their growth-phase-dependent behavior in C. marina using RNA-seq analysis. Finally, we speculate about the function of these genes and the ecological significance of 2-CysPrx, such as its involvement in circadian rhythms and the toxicity of C. marina to fish.

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Diurnal variations in expression of photosynthesis-related proteins in the harmful Raphidophyceae Chattonella marina var. antiqua

Cited by 6 publications

References 47 publications

Consequences of light spectra for pigment composition and gene expression in the cryptophyte Rhodomonas salina

Consequences of light spectra for pigment composition and gene expression in the cryptophyte Rhodomonas salina

Consequences of light spectra for pigment composition and gene expression in the cryptophyteRhodomonas salina

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

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