Diurnal variations in human urinary excretion of nicotinamide catabolites: effects of stress on the metabolism of nicotinamide

Okamoto, Hidemi; Ishikawa, Aya; Yoshitake, Yutaka; Kodama, Naoko; Nishimuta, Mamoru; Fukuwatari, Tsutomu; Shibata, Katsumi

doi:10.1093/ajcn/77.2.406

Cited by 56 publications

(60 citation statements)

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“…These data show a good agreement with those previously reported using different assay methods [15][16][17][18][22][23][24][25], indicating that the present assay is reliable and may be clinically applicable. Total renal clearance and active transport clearance for the three substances are shown in Table 3.…”

Section: Clinical Applicationsupporting

confidence: 92%

Simultaneous determination of N1-methylnicotinamide, l-carnitine, and creatinine in human plasma and urine by liquid chromatography with mass spectrometry for assessing the activities of multiple renal cationic transporters

Nobuoka

Ogawa

Echizen

2014

Journal of Chromatography B

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Section: Clinical Applicationsupporting

confidence: 92%

Simultaneous determination of N1-methylnicotinamide, l-carnitine, and creatinine in human plasma and urine by liquid chromatography with mass spectrometry for assessing the activities of multiple renal cationic transporters

Nobuoka

Ogawa

Echizen

2014

Journal of Chromatography B

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“…Moreover, we have not found any direct correlation between plasma Met2PY concentration and a total body mass of CRF children (not shown). To avoid the effect of diurnal variations in plasma Met2PY concentration [19], blood was drawn in the morning between 7 and 8 a.m. Since oxidative stress does occur in CRF patients [20,21], the increased DNA damage and consequently PARP-1 activation and increased Met2PY production cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of poly(ADP-ribose) polymerase inhibitors in children with chronic renal failure

et al. 2006

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Nicotinamide, N-methyl-2-pyridone-5-carboxamide (Met2PY) and N-methyl-4-pyridone-3-carboxamide (Met4PY) are biological metabolites of the intracellular coenzyme nicotinamide adenine dinucleotide (NAD) that can potentially inhibit poly(ADP-ribose) polymerase 1 (PARP-1; DNA repair enzyme). Our research was aimed at establishing whether chronic renal failure (CRF) in children leads to the elevation of plasma NAD metabolites sufficient to inhibit PARP-1 activity. Nicotinamide, Met2PY and Met4PY plasma and erythrocyte concentrations were measured in 25 children with CRF and in 19 healthy children. The effect of these NAD metabolites on PARP-1 activity was studied in vitro. We found that plasma concentration of all NAD metabolites (nicotinamide, Met2PY, Met4PY) in children with CRF could reach the concentration of 2, 30 and 10 microM as compared to 0.2, 1 and 0.5 microM, respectively, in healthy children. The concentration of nicotinamide metabolites correlated positively with plasma creatinine concentration and negatively with creatinine clearance in children with CRF. We found that Met2PY, Met4PY and nicotinamide inhibited in vitro PARP-1 activity with IC50 values of 2.1, 0.18 and 0.12 mM, respectively. Our data indicate that NAD metabolites accumulate in plasma of children with CRF and their combined effect could lead to the inhibition of PARP-1 activity. NAD metabolites could be particularly harmful in children due to higher DNA turnover than in adults.

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“…We reported a diurnal variation in the NAD and NADP catabolites N 1 -methylnicotinamide, N 1 -methyl-2-pyridone-5-carboxamide, and N 1 -methyl-4-pyridone-3-carboxamide was observed ( 1 ) and an elevation of blood NAD level after moderate exercise ( 2 ). In the present study, we examined whether NAD and NADP in blood also showed the diurnal variation.…”

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confidence: 81%

Consideration of Diurnal Variations in Human Blood NAD and NADP Concentrations

Fukuwatari

Shibata

2009

J Nutr Sci Vitaminol

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SummaryThe sum of the urinary excretion of nicotinamide and its catabolites, which are metabolites of NAD and NADP, were observed to have clear diurnal variations in human urine. Then, we examined whether NAD and NADP in blood also showed the diurnal variation. All subjects were housed in the same facility and given the same diet during the experiment. In addition, we examined whether diurnal variations were affected by the intakes of dietary nicotinamide or not. As a result, neither the NAD nor the NADP content of the blood shows the diurnal variation regardless of the administered amount of nicotinamide. The concentrations of NAD and NADP did not increase according to the intake of nicotinamide. The existence of a mechanism by which NAD and the NADP levels of the blood are constantly maintained by the adjustment of the amount of excretion to the urinary bladder, was suggested. Key Words diurnal variation, NAD, blood, human, vitaminWe reported a diurnal variation in the NAD and NADP catabolites N 1 -methylnicotinamide, N 1 -methyl-2-pyridone-5-carboxamide, and N 1 -methyl-4-pyridone-3-carboxamide was observed ( 1 ) and an elevation of blood NAD level after moderate exercise ( 2 ). In the present study, we examined whether NAD and NADP in blood also showed the diurnal variation. In addition, we examined whether diurnal variations were affected by the intakes of dietary nicotinamide or not.Ten healthy male Japanese college students participated in the present experiment. They did not have regular use of medications or dietary supplements, or habitual alcohol or cigarette consumption. Their age, body weight, height and body mass index (mean Ϯ SD) were 22.1 Ϯ 2.3 y old, 63.6 Ϯ 5.2 kg, 174.0 Ϯ 4.6 cm, and 21.0 Ϯ 1.6 kg/m 2

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Diurnal variations in human urinary excretion of nicotinamide catabolites: effects of stress on the metabolism of nicotinamide

Abstract: Diurnal variations in nicotinamide metabolism were found in these women. The biosynthesis of nicotinamide from tryptophan seemed to be increased by cold exposure.

Cited by 56 publications

References 14 publications

Simultaneous determination of N1-methylnicotinamide, l-carnitine, and creatinine in human plasma and urine by liquid chromatography with mass spectrometry for assessing the activities of multiple renal cationic transporters

Simultaneous determination of N1-methylnicotinamide, l-carnitine, and creatinine in human plasma and urine by liquid chromatography with mass spectrometry for assessing the activities of multiple renal cationic transporters

Accumulation of poly(ADP-ribose) polymerase inhibitors in children with chronic renal failure

Consideration of Diurnal Variations in Human Blood NAD and NADP Concentrations

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