Diurnal Variations in Release of Phytosiderophores and in Concentrations of Phytosiderophores and Nicotianamine in Roots and Shoots of Barley

Walter, A.; Pich, Axel; Scholz, G.; Marschner, H.; Römheld, Volker

doi:10.1016/s0176-1617(11)81505-1

Cited by 16 publications

(18 citation statements)

References 24 publications

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“…In barley, a diurnal pattern is observed wherein root PS (DMA, MA and epiHMA) concentrations are reduced during periods of heightened PS secretions (Kawai et al 1993; Nagasaka et al 2009; Walter et al 1995). In the current study, Fe deficiency induced a 1.5-fold increase in root DMA concentrations and a 2.4-fold increase in DMA secretions in rice plants (Fig.…”

Section: Discussionmentioning

confidence: 99%

Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice

Selby‐Pham

Lutz

Moreno-Moyano

et al. 2017

Rice

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BackgroundRice (Oryza sativa L.) is highly susceptible to iron (Fe) deficiency due to low secretion levels of the mugineic acid (MA) family phytosiderophore (PS) 2′-deoxymugineic acid (DMA) into the rhizosphere. The low levels of DMA secreted by rice have proved challenging to measure and, therefore, the pattern of DMA secretion under Fe deficiency has been less extensively studied relative to other graminaceous monocot species that secrete high levels of PS, such as barley (Hordeum vulgare L.).ResultsGene expression and metabolite analyses were used to characterise diurnal changes occurring during the Fe deficiency response of rice. Iron deficiency inducible genes involved in root DMA biosynthesis and secretion followed a diurnal pattern with peak induction occurring 3–5 h after the onset of light; a result consistent with that of other Strategy II plant species such as barley and wheat. Furthermore, triple quadrupole mass spectrometry identified 3–5 h after the onset of light as peak time of DMA secretion from Fe-deficient rice roots. Metabolite profiling identified accumulation of amines associated with metal chelation, metal translocation and plant oxidative stress responses occurring with peak induction 10–12 h after the onset of light.ConclusionThe results of this study confirmed that rice shares a similar peak time of Fe deficiency associated induction of DMA secretion compared to other Strategy II plant species but has less prominent daily fluctuations of DMA secretion. It also revealed metabolic changes associated with the remediation of Fe deficiency and mitigation of damage from resulting stress in rice roots. This study complements previous studies on the genetic changes in response to Fe deficiency in rice and constitutes an important advance towards our understanding of the molecular mechanisms underlying the rice Fe deficiency response.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-017-0152-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice

Selby‐Pham

Lutz

Moreno-Moyano

et al. 2017

Rice

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“…After 8 days of hydroponic culture, root exudates released by Fe‐deficient barley plants were collected by transferring plants to 100 ml of aerated distilled water (pH 6.0) for 4 h during the morning (a period of high PS release). Root exudates containing PS [mainly epi‐hydroxymugineic acid (epi‐HMA); Walter et al ] were filtered through a Whatman WCN 0.2 µm membrane filter and then stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Iron allocation in leaves of Fe‐deficient cucumber plants fed with natural Fe complexes

Zanin

Tomasi

Rizzardo

et al. 2014

Physiologia Plantarum

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Iron (Fe) sources available for plants in the rhizospheric solution are mainly a mixture of complexes between Fe and organic ligands, including phytosiderophores (PS) and water-extractable humic substances (WEHS). In comparison with the other Fe sources, Fe-WEHS are more efficiently used by plants, and experimental evidences show that Fe translocation contributes to this better response. On the other hand, very little is known on the mechanisms involved in Fe allocation in leaves. In this work, physiological and molecular processes involved in Fe distribution in leaves of Fe-deficient Cucumis sativus supplied with Fe-PS or Fe-WEHS up to 5 days were studied combining different techniques, such as radiochemical experiments, synchrotron micro X-ray fluorescence, real-time reverse transcription polymerase chain reaction and in situ hybridization. In Fe-WEHS-fed plants, Fe was rapidly (1 day) allocated into the leaf veins, and after 5 days, Fe was completely transferred into interveinal cells; moreover, the amount of accumulated Fe was much higher than with Fe-PS. This redistribution in Fe-WEHS plants was associated with an upregulation of genes encoding a ferric(III) -chelate reductase (FRO), a Fe(2+) transporter (IRT1) and a natural resistance-associated macrophage protein (NRAMP). The localization of FRO and IRT1 transcripts next to the midveins, beside that of NRAMP in the interveinal area, may suggest a rapid and efficient response induced by the presence of Fe-WEHS in the extra-radical solution for the allocation in leaves of high amounts of Fe. In conclusion, Fe is more efficiently used when chelated to WEHS than PS and seems to involve Fe distribution and gene regulation of Fe acquisition mechanisms operating in leaves.

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“…Diurnal patterns of exudation which derive from the change of availability of photosynthates in the plant as shown for instance for citrate release by proteoid roots (Keerthisinghe et al 1998, Dessureault-Rompré et al 2007) are also possibly implied in a diurnal pattern of P acquisition. In this respect, the most remarkable circadian pattern is that described for phytosiderophore release in iron deficient graminaceous plant species (Walter et al 1995;Reichman and Parker 2007). A distinct peak of secretion occurs between 3 and 6 h after the onset of light, which is quite a more efficient strategy for efficient Fe acquisition than evenly releasing such ligands at a lower flux all day long (Robin et al 2008).…”

Section: Underlying Processesmentioning

confidence: 96%

Rhizosphere: biophysics, biogeochemistry and ecological relevance

Bengough²,

et al. 2009

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Life on Earth is sustained by a small volume of soil surrounding roots, called the rhizosphere. The soil is where most of the biodiversity on Earth exists, and the rhizosphere probably represents the most dynamic habitat on Earth; and certainly is the most important zone in terms of defining the quality and quantity of the Human terrestrial food resource. Despite its central importance to all life, we know very little about rhizosphere functioning, and have an extraordinary ignorance about how best we can manipulate it to our advantage. A major issue in research on rhizosphere processes is the intimate connection between the biology, physics and chemistry of the system which exhibits astonishing spatial and temporal heterogeneities. This review considers the unique biophysical and biogeochemical properties of the rhizosphere and draws some connections between them. Particular emphasis is put on how underlying processes affect rhizosphere ecology, to generate highly heterogeneous microenvironments. Rhizosphere ecology is driven by a combination of the physical architecture of the soil matrix, coupled with the spatial and temporal distribution of rhizodeposits, protons, gases, and the role of roots as sinks for water and nutrients. Consequences for plant growth and whole-system ecology are considered. The first sections address the physical architecture and soil strength of the rhizosphere, drawing their relationship with key functions such as the movement and storage of elements and water as well as the ability of roots to explore the soil and the definition of diverse habitats for soil microorganisms. The distribution of water and its accessibility in the rhizosphere is considered in detail, with a special emphasis on spatial and temporal dynamics and heterogeneities. The physical architecture and water content play a key role in determining the biogeochemical ambience of the rhizosphere, via their effect on partial pressures of O 2 and CO 2 , and thereby on redox potential and pH of the rhizosphere, respectively. We address the Plant Soil

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Diurnal Variations in Release of Phytosiderophores and in Concentrations of Phytosiderophores and Nicotianamine in Roots and Shoots of Barley

Cited by 16 publications

References 24 publications

Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice

Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice

Iron allocation in leaves of Fe‐deficient cucumber plants fed with natural Fe complexes

Rhizosphere: biophysics, biogeochemistry and ecological relevance

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