Diurnal variations of urinary enzyme excretion

Maruhn, D; Strozyk, K.; Gielow, L.; Bock, K. D.

doi:10.1016/0009-8981(77)90362-x

Cited by 46 publications

(20 citation statements)

References 15 publications

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“…In one case, the γ-glutamyltransferase excretion pattern of volunteer 3 (table 5), the fit of a cosine wave did not result in a significant amplitude. Four of the volunteers (5,7,8,14) displayed a clear circaseptan (7 ± 1 days) rhythm in the dipeptidyl peptidase IV excretion, but only two in the excretion of γ-glutamyltransferase (4,13) and alanine aminopeptidase (1,8). The dominant period length can be very different for the enzymuria of dipeptidyl peptidase IV, γ-glutamyltransferase and alanine aml· nopeptidase in one and the same person (e. g. volunteers 2, 6).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Infradian Biorhythms of Enzymuria in Man?

Burchardt¹,

Winkler²,

Klagge³

et al. 1988

Clinical Chemistry and Laboratory Medicine

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Summary:The temporal courses of dipeptidyl peptidase IV γ-glutamyltransferase and alanine aminopeptidase were followed over 70 days in the morning urine of 15 healthy persons. Subsequent to basic statistical analysis a two-step procedure was performed, including spectral analysis and the fit of a cosine function by non-linear regression. The excretion of the 3 enzymes followed an infradian biorhythm with a mean period length of 10.04 for dipeptidyl peptidase IV, 13.34 for γ-glutamyltransferase and 10.17 for alanine aminopeptidase. In addition to the basic rhythmic process described by the fitted cosine functions, in most of the enzyme patterns steap peaks of very high excretory activity appeared which was verified in repeated measurements.These infradian biorhythms with changes in the range of 100% and more, as well as their interindividual variations, have to be considered in assessing the excretion of enzymes.70 days in 15 volunteers of both sexes, 26 to 55 years Urine enzymes are now widely accepted as sensitive old. The detection of relevant periodicities in the indicators for the early detection of renal alterations individual patterns of all volunteers was ensured by (see I.e. (1, 2) for references). Since Gr tsch et al. (3) applying spectral analysis and non-linear regression. reported, for the rat, the existence of biological rhythms in the excretion of y^glutajuyltransferase, Nacetyl^jD-glucosaminidase and alanine aminopepti-Materials and Methods dase with dominant periods in the infradian range of The urinary enzyme excretion was determined once daily on 70 7 and 9 days, respectively, it has been obvious, that consecutive days in 15 healthy adults (8 women, 7 men, mean ι.j ^ 11 j 't_ j · ·,· i t />· /rx age 39.4, range 26-55 years). Urines were collected between 5 beyond the well described circadian rhythms (4, 5) and 9 a m and imme di at eiy centrifuged (15 min, 1800*).enzymuria can also be subjected to infradian rhythmic Subsequent to centrifugation the samples were filtered on Sechanges. phadex G 50 (medium) with 154 mmol/1 NaCl solution (6). The fraction with a relative molecular mass M r > 10 000 was stored Therefore, our study was designed to clarify whether at ~2 0 °c after addin ? sodium ^^ solution ( 15 mmol/1).or not such infradian changes are also present in man. γ-Glutamyltransferase activity was pursued at 37 °C for 5 min, The urinary excretion of γ-glutamyltransferase (EC usin a continuous method. The reaction mixture contained 4.0 ο ο >· > o\ ι · · s* /c^ ο A \4 o\ j mmol/1 glutamyl-4-nitroanilide and 101 mmol/1 glycylglycine 2.3.2.2), alanine aminopeptidase (EC 3.4.11.2) and bufTer ' p § 820 fa OQ. Spectrophotometric reading were taken dipeptidylpeptidase IV (EC 3.4.14.5) was followed for at 405 nm.

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Section: Resultsmentioning

confidence: 99%

“…beyond the well described circadian rhythms (4,5) and 9 a m and imme di at eiy centrifuged (15 min, 1800*). enzymuria can also be subjected to infradian rhythmic Subsequent to centrifugation the samples were filtered on Sechanges.…”

mentioning

confidence: 99%

Infradian Biorhythms of Enzymuria in Man?

Burchardt¹,

Winkler²,

Klagge³

et al. 1988

Clinical Chemistry and Laboratory Medicine

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“…In contrast to the measured CCr, which suffers from these variations, prediction methods by definition are not influenced because in these methods serum creatinine (SCr) is the only variable parameter for a given individual. The diurnal variation of SCr has been reported to be only 10-15% in an individual (Addis et al, 1950;Doolan et al, 1962;Statland et al, 1973;Winkel et al, 1975), while the urinary creatinine excretion can vary between 30 and 400% (Clark et al, 1951;Pasternack & Kuhlback, 1971;Greenblatt et al, 1976;Maruhn et al, 1977). Because of these large variations in urinary creatinine excretion, measured CCr will vary accordingly.…”

Section: Creatinine Clearance Different Methods Of Determinationmentioning

confidence: 98%

Creatinine clearance, different methods of determination.

Thomis¹,

Soep²,

Hallynck³

et al. 1982

Brit J Clinical Pharma

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“…The urine specimens were exclusively sampled for the study of enzymuria and neopterin excretion. Whenever possible, the measurements were carried out using the second midstream morning urine, in order to avoid influences of diurnal variations on enzyme excretion [10]. The enzyme activity was measured within 3 h of urine collection.…”

Section: Methodsmentioning

confidence: 99%

Urinary N-Acetyl-β-<i>D</i>-Glucosaminidase and Neopterin Aid in the Diagnosis of Rejection and Acute Tubular Necrosis in Initially Nonfunctioning Kidney Grafts

Kotanko

Margreiter

Pfaller

2000

Nephron

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Aim: The study aimed at investigating urinary neopterin, a marker of cellular immune response, and urinary N-acetyl-β-D-glucosaminidase (NAG), a marker of tubular damage, as noninvasive means to differentiate between acute tubular necrosis (ATN) and rejection in initially nonfunctioning (INF) human renal transplants. Methods: Seventy-two renal transplant patients were studied. Forty-five of them experienced an uncomplicated early posttransplant course, 27 patients suffered from INF. Twenty-two patients experienced ATN, 5 patients had a total of six biopsy-proven rejections. The NAG activity was measured by a colorimetric assay, neopterin by high-performance liquid chromatography. Receiver operating characteristics (ROC) analysis was applied to compute diagnostic performance and an optimal discriminating threshold. Results: Demographic characteristics (age, gender, cold and warm ischemia periods, HLA mismatches) and posttransplant urinary NAG and neopterin excretions did not differ between ATN and rejection groups. Both urinary NAG and neopterin excretions were lower in the control group (NAG 1.8 ± 1.0 U/mmol urinary creatinine; neopterin 270 ± 126 nmol/mmol urinary creatinine; mean ± SD) as compared with the ATN group (NAG 12 ± 10 U/mmol, p < 0.001 vs. control group; neopterin 303 ± 195 nmol/mmol, n.s.) and the rejection group (NAG 7 ± 8 U/mmol, p < 0.01; neopterin 508 ± 419 nmol/mmol, p < 0.01). The ratio of urinary neopterin to NAG excretion (uNNR; dimension nmol neopterin/U NAG activity) increased during rejections as compared with ATN (139 ± 74 vs. 50 ± 38 nmol/U, p < 0.01). The area under the ROC curve for uNNR was 0.88 ± 0.07 (p < 0.001). Applying a ROC-estimated optimal discriminator of uNNR (80 nmol/U), 16 patients with ATN and all six rejection episodes were classified correctly. Conclusion: The uNNR provides a noninvasive means to aid in the differential diagnosis of rejection and ATN in INF human renal transplants.

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Diurnal variations of urinary enzyme excretion

Cited by 46 publications

References 15 publications

Infradian Biorhythms of Enzymuria in Man?

Infradian Biorhythms of Enzymuria in Man?

Creatinine clearance, different methods of determination.

Urinary N-Acetyl-β-<i>D</i>-Glucosaminidase and Neopterin Aid in the Diagnosis of Rejection and Acute Tubular Necrosis in Initially Nonfunctioning Kidney Grafts

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