Diuron and diazinon alter the behavior of zebrafish embryos and larvae in the absence of acute toxicity

Velki, Mirna; Paolo, Carolina Di; Nelles, Jonas; Seiler, Thomas‐Benjamin; Hollert, Henner

doi:10.1016/j.chemosphere.2017.04.017

Cited by 93 publications

(55 citation statements)

References 52 publications

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“…Investigating changes in behavior, especially locomotor activity, has become a widely accepted method for assessing compounds of interest for toxicological and neurodevelopmental issues [91][92][93][94][95][96][97][98]. Zebrafish are an excellent species for modeling neurobehaviors associated with human disease because the neurotransmitter systems are well described and there is conserved function with respect to the human nervous system [99][100][101][102].…”

Section: Discussionmentioning

confidence: 99%

Exposure of Larval Zebrafish to the Insecticide Propoxur Induced Developmental Delays that Correlate with Behavioral Abnormalities and Altered Expression of hspb9 and hspb11

Shields

Hales

Ranspach

et al. 2019

Toxics

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Recent studies suggest that organophosphates and carbamates affect human fetal development, resulting in neurological and growth impairment. However, these studies are conflicting and the extent of adverse effects due to pesticide exposure warrants further investigation. In the present study, we examined the impact of the carbamate insecticide propoxur on zebrafish development. We found that propoxur exposure delays embryonic development, resulting in three distinct developmental stages: no delay, mild delay, or severe delay. Interestingly, the delayed embryos all physically recovered 5 days after exposure, but behavioral analysis revealed persistent cognitive deficits at later stages. Microarray analysis identified 59 genes significantly changed by propoxur treatment, and Ingenuity Pathway Analysis revealed that these genes are involved in cancer, organismal abnormalities, neurological disease, and hematological system development. We further examined hspb9 and hspb11 due to their potential roles in zebrafish development and found that propoxur increases expression of these small heat shock proteins in all of the exposed animals. However, we discovered that less significant increases were associated with the more severely delayed phenotype. This raises the possibility that a decreased ability to upregulate these small heat shock proteins in response to propoxur exposure may cause embryos to be more severely delayed.

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Section: Discussionmentioning

confidence: 99%

Exposure of Larval Zebrafish to the Insecticide Propoxur Induced Developmental Delays that Correlate with Behavioral Abnormalities and Altered Expression of hspb9 and hspb11

Shields

Hales

Ranspach

et al. 2019

Toxics

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“…As a result, the concentration of pesticides in tropical environments has constantly increased, causing a decline in species diversity. Although pesticides have certain beneficial effects for agricultural crops, their use can cause a wide range of toxic effects on different nontarget organisms [4].…”

Section: Introductionmentioning

confidence: 99%

“…Diazinon (O,O-diethyl-O-[2-isopropyl-6-methyl-4pyrimidinyl] phosphorothioate) is a nonsystemic organophosphate insecticide. It was commonly used for insert control in agricultural crops [4,5]. e toxicity of diazinon results from inhibiting the enzyme acetylcholinesterase (AChE), leading to the accumulation of neurotransmitters and altered signal transmission in chemical synapses [6].…”

Section: Introductionmentioning

confidence: 99%

“…e toxicity of diazinon results from inhibiting the enzyme acetylcholinesterase (AChE), leading to the accumulation of neurotransmitters and altered signal transmission in chemical synapses [6]. In the domestic and international markets, there are more than 500 registered products that contain diazinon as the active substance [4]. Due to its widespread use, diazinon is frequently found in freshwater ecosystems [7].…”

Section: Introductionmentioning

confidence: 99%

“…Diazinon generates high acute toxicity to a wide variety of aquatic organisms, leading to a wide range of sublethal biochemical effects, damage to specific target organs and tissues, and adverse ecological impacts. e toxicity of diazinon has extensively studied in fish [4,[9][10][11] and crustaceans [6,12,13], and it has been reported to be moderately toxic to early life stages of zebrafish [4]. e 96 h LC 50 values ranging from 0.32 to 1.53 mg•L −1 for larval and from 2.2 to 10.3 mg•L −1 for adult of several fish have already been recorded [10].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Diazinon Toxicity to Temperate and Tropical Freshwater Daphnia Species

Pham

Bùi

2018

Journal of Chemistry

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The presence of pesticides in water bodies presents unique challenges to the ecosystem and all the life forms. Biological methods have been widely used to examine the toxic effects of various toxicants including pesticides. The present study aims at determining the adverse effects of diazinon, a nonsystemic organophosphate insecticide, on two cladoceran species including the temperate Daphnia magna (D. magna) and the tropical Daphnia lumholtzi (D. lumholtzi). The 48 h LC50 values demonstrated higher toxicity of diazinon for D. lumholtzi at a concentration of 3.41 µg·L−1 compared to D. magna at a concentration of 4.63 µg·L−1. After 14 days of exposure to diazinon, the survival capacity as well as the reproduction potential of the two cladoceran species clearly reduced and their rate of population increase (RPI) decreased at concentrations >0.1 µg·L−1. The present study indicated that the tropical cladoceran (D. lumholtzi) was more sensitive than the temperate D. magna. Therefore, it could be used as an indicator for toxicity assessment in tropical environments. The presence of diazinon in water bodies can be associated with significant risk to aquatic organisms.

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Aconitine disrupts serotonin neurotransmission via 5‐hydroxytryptamine receptor in zebrafish embryo

Chen

Wang

et al. 2020

J of Applied Toxicology

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Medicinal plants of the genus Aconitum are one of the most commonly used herbs in traditional medicine in East Asia to treat conditions related to the heart, pain, or inflammation. However, these herbs are also dangerous as accidental poisoning due to misuse is a recurring issue. These plants contain a number of diester‐diterpenoid alkaloid compounds and aconitine is the most abundant and active one. This study investigated neurotoxicity of aconitine to zebrafish embryos in early development in relation to serotonin regulation. Experimental results showed that aconitine exposure (1, 10, and 100 μM) increased frequency of coiling behavior in zebrafish embryos in a dose‐dependent manner and this effect can be triggered by either exposure to 5‐hydroxytryptamine 1A (5‐HT1A) receptor agonist (±)‐8‐hydroxy‐2‐(dipropylamino)tetralin (8‐OH‐DPAT) or overexpression of serotonin receptor 5‐htr1ab. At the same time, coiling behavior caused by aconitine exposure could be rescued by co‐exposure to 5‐HT1A receptor antagonist WAY‐100635 Maleate (WAY100635) and knockdown of 5‐htr1ab using morpholino. Exposure to aconitine also significantly increased serotonin receptor 5‐htr1ab and 5‐htr1bd gene expression at 24 h post fertilization (hpf), but decreased their expression and protein expression of the serotonin receptor at 96 hpf with the high dose. These results suggest that neurotoxicity caused by aconitine is mediated through the 5‐HT receptor.

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Diuron and diazinon alter the behavior of zebrafish embryos and larvae in the absence of acute toxicity

Cited by 93 publications

References 52 publications

Exposure of Larval Zebrafish to the Insecticide Propoxur Induced Developmental Delays that Correlate with Behavioral Abnormalities and Altered Expression of hspb9 and hspb11

Exposure of Larval Zebrafish to the Insecticide Propoxur Induced Developmental Delays that Correlate with Behavioral Abnormalities and Altered Expression of hspb9 and hspb11

Comparison of Diazinon Toxicity to Temperate and Tropical Freshwater Daphnia Species

Aconitine disrupts serotonin neurotransmission via 5‐hydroxytryptamine receptor in zebrafish embryo

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