Divergence in δ13C of dark respired CO2 and bulk organic matter occurs during the transition between heterotrophy and autotrophy in Phaseolus vulgaris plants

Bathellier, Camille; Badeck, Franz-W.; Couzi, Philippe; Harscoët, Sébastien; Mauve, Caroline; Ghashghaie, Jaleh

doi:10.1111/j.1469-8137.2007.02246.x

Cited by 89 publications

(142 citation statements)

References 68 publications

Supporting

Mentioning

126

Contrasting

Order By: Relevance

“…Accordingly, the δ 13 C of CO 2 produced during PPP is relatively depleted in 13 C compared to respiratory substrate. High PPP activity reported for roots could explain the 13 C depletion in root-respired CO 2 (Bathellier et al, 2008) compared to 13 C-enriched CO 2 respired by leaves (Duranceau et al, 1999;Tcherkez et al, 2003).…”

Section: Post-carboxylation Fractionationmentioning

confidence: 94%

Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review

Brüggemann¹,

et al. 2011

View full text Add to dashboard Cite

Abstract. The terrestrial carbon (C) cycle has received increasing interest over the past few decades, however, there is still a lack of understanding of the fate of newly assimilated C allocated within plants and to the soil, stored within ecosystems and lost to the atmosphere. Stable carbon isotope studies can give novel insights into these issues. In this review we provide an overview of an emerging picture of plant-soil-atmosphere C fluxes, as based on C isotopeCorrespondence to: N. Brüggemann (n.brueggemann@fz-juelich.de) studies, and identify processes determining related C isotope signatures. The first part of the review focuses on isotopic fractionation processes within plants during and after photosynthesis. The second major part elaborates on plantinternal and plant-rhizosphere C allocation patterns at different time scales (diel, seasonal, interannual), including the speed of C transfer and time lags in the coupling of assimilation and respiration, as well as the magnitude and controls of plant-soil C allocation and respiratory fluxes. Plant responses to changing environmental conditions, the functional relationship between the physiological and phenological status of plants and C transfer, and interactions between Published by Copernicus Publications on behalf of the European Geosciences Union. 3458 N. Brüggemann et al.: Plant-soil-atmosphere C fluxes C, water and nutrient dynamics are discussed. The role of the C counterflow from the rhizosphere to the aboveground parts of the plants, e.g. via CO 2 dissolved in the xylem water or as xylem-transported sugars, is highlighted. The third part is centered around belowground C turnover, focusing especially on above-and belowground litter inputs, soil organic matter formation and turnover, production and loss of dissolved organic C, soil respiration and CO 2 fixation by soil microbes. Furthermore, plant controls on microbial communities and activity via exudates and litter production as well as microbial community effects on C mineralization are reviewed. A further part of the paper is dedicated to physical interactions between soil CO 2 and the soil matrix, such as CO 2 diffusion and dissolution processes within the soil profile. Finally, we highlight state-of-the-art stable isotope methodologies and their latest developments. From the presented evidence we conclude that there exists a tight coupling of physical, chemical and biological processes involved in C cycling and C isotope fluxes in the plant-soil-atmosphere system. Generally, research using information from C isotopes allows an integrated view of the different processes involved. However, complex interactions among the range of processes complicate or currently impede the interpretation of isotopic signals in CO 2 or organic compounds at the plant and ecosystem level. This review tries to identify present knowledge gaps in correctly interpreting carbon stable isotope signals in the plant-soil-atmosphere system and how future research approaches could contribute to closing these gaps.

show abstract

Section: Post-carboxylation Fractionationmentioning

confidence: 94%

Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review

Brüggemann¹,

et al. 2011

View full text Add to dashboard Cite

show abstract

“…1 without the pot) were included to correct for possible contamination from carbonate in the NaOH stock solution and from sample handling (Cheng et al 2003). Therefore, unlike the gas exchange system or the direct gas sampling system which measured CO 2 produced during a short time period (<30 min) used in previous studies (Bathellier et al 2008;Gessler et al 2007;Klumpp et al 2005;Moyes et al 2010), our closed-circulation CO 2 trapping system rendered a more integrative measurement of 13 C fractionation during rhizosphere respiration.…”

Section: Comparison With Previous Studiesmentioning

confidence: 99%

“…Most published studies on 13 C fractionation during root respiration were based on snapshot measurements (minutes to hours) of the δ 13 C value of respiratory CO 2 from excised roots (Gessler et al 2007;Wegener et al 2010) or roots grown in sand or nutrient solution without the presence of soil microorganisms (Bathellier et al 2008;Klumpp et al 2005), and thus did not include the rhizomicrobial respiration by rhizosphere microorganisms utilizing materials released from live roots. In order to partition total soil respiration into rhizosphere respiration that includes both root respiration and rhizomicrobial respiration (root-derived, autotrophic respiration) and microbial decomposition of soil organic matter (soilderived, heterotrophic respiration) using a two endmember isotope mixing model (Cheng 1996), we need to know δ 13 C values of rhizosphere respiration integrated over days or seasons.…”

Section: Introductionmentioning

confidence: 99%

13C isotope fractionation during rhizosphere respiration of C3 and C4 plants

Zhu

Cheng

2011

Plant Soil

View full text Add to dashboard Cite

Stable carbon isotopes are used extensively to partition total soil CO 2 efflux into root-derived rhizosphere respiration or autotrophic respiration and soil-derived heterotrophic respiration. However, it remains unclear whether CO 2 from rhizosphere respiration has the same δ 13 C value as root biomass. Here we investigated the magnitude of 13 C isotope fractionation during rhizosphere respiration relative to root biomass in six plant species. Plants were grown in a carbon-free sand-perlite medium inoculated with microorganisms from a farm soil for 62 days inside a greenhouse. We measured the δ 13 C value of rhizosphere respiration using a closed-circulation 48-hour CO 2 trapping method during 40~42 and 60~62 days after sowing. We found a consistent depletion in 13 C (0.9~1.7‰) of CO 2 from rhizosphere respiration relative to root biomass in three C 3 species (Glycine max L. Merr., Helianthus annuus L. and Triticum aestivum L.), but a relatively large depletion in 13 C (3.7~7.0‰) in three C 4 species (Amaranthus tricolor L., Sorghum bicolor (L.) Moench and Zea mays L. ssp. mays). Overall, our results indicate that CO 2 from rhizosphere respiration is more 13 C-depleted than root biomass. Therefore, accounting for this 13 C fractionation is required for accurately partitioning total soil CO 2 efflux into root-derived and soil-derived components using natural abundance stable carbon isotope methods.

show abstract

“…Increased activity of the oxidative pentose phosphate pathway (PPP), which decarboxylates the 13 C depleted C-1 position of glucose, can be significant in roots (DieuaideNoubhani et al, 1995;Bathellier et al, 2008Bathellier et al, , 2009). Thus, temporal changes in the partitioning of carbon originating from glucose-6-phosphate between glycolysis and PPP might also be responsible for diel variations in δ 13 C res .…”

Section: Fractionation Driven Variations (M2)mentioning

confidence: 99%

Diel variations in the carbon isotope composition of respired CO2 and associated carbon sources: a review of dynamics and mechanisms

Werner

Geßler

2011

Biogeosciences

View full text Add to dashboard Cite

Abstract. Recent advances have improved our methodological approaches and theoretical understanding of postphotosynthetic carbon isotope fractionation processes. Nevertheless we still lack a clear picture of the origin of shortterm variability in δ 13 C of respired CO 2 (δ 13 C res ) and organic carbon fractions on a diel basis. Closing this knowledge gap is essential for the application of stable isotope approaches for partitioning ecosystem respiration, tracing carbon flow through plants and ecosystems and disentangling key physiological processes in carbon metabolism of plants. In this review we examine the short-term dynamics in δ 13 C res and putative substrate pools at the plant, soil and ecosystem scales and discuss mechanisms, which might drive diel δ 13 C res dynamics at each scale. Maximum reported variation in diel δ 13 C res is 4.0, 5.4 and 14.8 ‰ in trunks, roots and leaves of different species and 12.5 and 8.1 ‰ at the soil and ecosystem scale in different biomes. Temporal variation in post-photosynthetic isotope fractionation related to changes in carbon allocation to different metabolic pathways is the most plausible mechanistic explanation for observed diel dynamics in δ 13 C res . In addition, mixing of component fluxes with different temporal dynamics and isotopic compositions add to the δ 13 C res variation on the soil and ecosystem level. Understanding short-term variations in δ 13 C res is particularly important for ecosystem studies, since δ 13 C res contains information on the fate of respiratory substrates, and may, therefore, provide a non-intrusive way to identify changes in carbon allocation patterns.

show abstract

Divergence in δ¹³C of dark respired CO₂ and bulk organic matter occurs during the transition between heterotrophy and autotrophy in Phaseolus vulgaris plants

Cited by 89 publications

References 68 publications

Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review

Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review

13C isotope fractionation during rhizosphere respiration of C3 and C4 plants

Diel variations in the carbon isotope composition of respired CO<sub>2</sub> and associated carbon sources: a review of dynamics and mechanisms

Contact Info

Product

Resources

About

Divergence in δ13C of dark respired CO2 and bulk organic matter occurs during the transition between heterotrophy and autotrophy in Phaseolus vulgaris plants

Cited by 89 publications

References 68 publications

Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review

Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review

13C isotope fractionation during rhizosphere respiration of C3 and C4 plants

Diel variations in the carbon isotope composition of respired CO&lt;sub&gt;2&lt;/sub&gt; and associated carbon sources: a review of dynamics and mechanisms

Contact Info

Product

Resources

About

Divergence in δ¹³C of dark respired CO₂ and bulk organic matter occurs during the transition between heterotrophy and autotrophy in Phaseolus vulgaris plants

Diel variations in the carbon isotope composition of respired CO<sub>2</sub> and associated carbon sources: a review of dynamics and mechanisms