Divergence of tRNA genes in chloroplast DNA of higher plants

Meeker, R.; Tewari, Κ. Κ.

doi:10.1016/0167-4781(82)90011-2

Cited by 12 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 1 lists the tRNA genes that have been sequenced, tRNA genes of ctDNA of higher plants are extremely homologous. Competition hybridization experiments between heterologous ctDNA and tRNAs has shown homology between the total tRNAs of various higher plants (29). Nucleotide sequence analysis has confirmed such homology.…”

Section: Discussionmentioning

confidence: 69%

Nucleotide sequences of transfer RNA genes in the Pisum sativum chloroplast DNA

Shapiro

Tewari

1986

Plant Mol Biol

View full text Add to dashboard Cite

Eight transfer RNA (tRNA) genes which were previously mapped to five regions of the Pisum sativum (pea) chloroplast DNA (ctDNA) have been sequenced. They have been identified as tRNA(Val)(GAC), tRNA(Asn)(GUU), tRNA(Arg)(ACG), tRNA(Leu)(CAA), tRNA(Tyr)(GUA), tRNA(Glu)(UUC), tRNA(His)(GUG), and tRNA(Arg)(UCU) by their anticodons and by their similarity to other previously identified tRNA genes from the chloroplast DNAs of higher plants or from E. gracilis. In addition,two other tRNA genes, tRNA(Gly) (UCC) and tRNA(Ile)(GAU), have been partially sequenced. The tRNA genes are compared to other known chloroplast tRNA genes from higher plants and are found to be 90-100% homologous. In addition there are similarities in the overall arrangement of the individual genes between different plants. The 5' flanking regions and the internal sequences of tRNA genes have been studied for conserved regions and consensus sequences. Two unusual features have been found: there is an apparent intron in the D-loop of the tRNA(Gly)(UCC), and the tRNA(Glu)(UUC) contains GATTC in its T-loop.

show abstract

Section: Discussionmentioning

confidence: 69%

Nucleotide sequences of transfer RNA genes in the Pisum sativum chloroplast DNA

Shapiro

Tewari

1986

Plant Mol Biol

View full text Add to dashboard Cite

show abstract

“…42 genes) in all three legumes. From a previous study on the number of Pisum chloroplast tRNA genes, approximately 42 genes were estimated by saturation-hybridization of total chloroplast tRNA to total chloroplast DNA [13,14]. The reason for having a higher copy number of some tRNA genes is not known.…”

Section: Comparison Of the Chloroplast Gene Maps Of Pisum Vicia And mentioning

confidence: 99%

Divergence of chloroplast gene organization in three legumes: Pisum sativum, Vicia faba and Phaseolus vulgaris

Crouse¹,

Mubumbila

Stummann³

et al. 1986

Plant Mol Biol

View full text Add to dashboard Cite

Isolated chloroplasts from Pisum sativum were found to contain at least 32 tRNA species. Hybridization of in vitro labeled, identified, chloroplast tRNAs to Pisum chloroplast DNA fragments revealed the locations of the tRNA genes on the circular chloroplast genome. Comparison of this gene map to the maps of Vicia faba and Phaseolus vulgaris showed that the chloroplast genomes of Pisum and Phaseolus are otherwise more closely related than either genome is to the chloroplast genome of Vicia. Furthermore, the results suggest how possible recombination events could be involved in the evolution of these three closely related, but divergent, chloroplast genomes.

show abstract

“…Chloroplasts play vital roles in the growth and development of plants, including the synthesis of nucleotides, amino acids, fatty acids, vitamins, phytohormones, and several other metabolites [15][16][17]. The chloroplast genome is a highly conserved, doublestranded circular molecule containing genes that encode tRNAs, rRNAs, and many proteins [18,19]. The semiautonomous and complete expression system of the plant plastid genome makes it a good material for evolutionary and genomics research [20,21].…”

Section: Introductionmentioning

confidence: 99%

Evolution and structural variations in chloroplast tRNAs in gymnosperms

et al. 2021

View full text Add to dashboard Cite

Background Chloroplast transfer RNAs (tRNAs) can participate in various vital processes. Gymnosperms have important ecological and economic value, and they are the dominant species in forest ecosystems in the Northern Hemisphere. However, the evolution and structural changes in chloroplast tRNAs in gymnosperms remain largely unclear. Results In this study, we determined the nucleotide evolution, phylogenetic relationships, and structural variations in 1779 chloroplast tRNAs in gymnosperms. The numbers and types of tRNA genes present in the chloroplast genomes of different gymnosperms did not differ greatly, where the average number of tRNAs was 33 and the frequencies of occurrence for various types of tRNAs were generally consistent. Nearly half of the anticodons were absent. Molecular sequence variation analysis identified the conserved secondary structures of tRNAs. About a quarter of the tRNA genes were found to contain precoded 3′ CCA tails. A few tRNAs have undergone novel structural changes that are closely related to their minimum free energy, and these structural changes affect the stability of the tRNAs. Phylogenetic analysis showed that tRNAs have evolved from multiple common ancestors. The transition rate was higher than the transversion rate in gymnosperm chloroplast tRNAs. More loss events than duplication events have occurred in gymnosperm chloroplast tRNAs during their evolutionary process. Conclusions These findings provide novel insights into the molecular evolution and biological characteristics of chloroplast tRNAs in gymnosperms.

show abstract

Divergence of tRNA genes in chloroplast DNA of higher plants

Cited by 12 publications

References 10 publications

Nucleotide sequences of transfer RNA genes in the Pisum sativum chloroplast DNA

Nucleotide sequences of transfer RNA genes in the Pisum sativum chloroplast DNA

Divergence of chloroplast gene organization in three legumes: Pisum sativum, Vicia faba and Phaseolus vulgaris

Evolution and structural variations in chloroplast tRNAs in gymnosperms

Contact Info

Product

Resources

About