Divergent effects of desferrioxamine on bacterial growth and characteristics

Eto, Daisei; Watanabe, Kenta; Saeki, Hisafumi; Oinuma, Ken-Ichi; Otani, Ko-ichi; Nobukuni, Megumi; Shiratori-Takano, Hatsumi; Takano, Hideaki; Beppu, Teruhiko; Uéda, Kenji

doi:10.1038/ja.2012.111

Cited by 21 publications

(12 citation statements)

References 10 publications

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“…9 This was the first observation suggesting that in Streptomyces species, these ironchelating compounds might have a much more complex and subtle role in triggering a wide range of cellular processes. 6,9 In line with these findings, Tierrafria and collaborators showed that the disruption of the desferrioxamine-binding receptor encoded by desE resulted in loss of sporulation in S. coelicolor. 10 Recently, co-culture experiments demonstrated that iron competition through siderophore piracy between actinomycetes prevented S. coelicolor from entering its sporulation program.…”

Section: Introductionmentioning

confidence: 74%

“…2 This suggests that the survival of a large percentage of microorganisms on Earth may depend on neighbouring siderophore-producing species. 2 Iron plays essential roles in house-keeping functions such as DNA replication, protein synthesis and respiration, and siderophore-mediated iron uptake is specifically associated with many other biological processes such as fungal sexual development 3 and germination, 4 plant growth promotion, 5 pilus formation, 6 and pathogen virulence. 7,8 In a study by Ueda and colleagues on terrestrial antibiotic-producing streptomycetes, it was reported that morphological development (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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Streptomyces coelicolor is an important model organism for developmental studies of filamentous GCrich actinobacteria. The genetic characterization of mutants of S. coelicolor blocked at the vegetative mycelium stage, the so-called bald (bld) mutants that are unable to erect spore-forming aerial hyphae, has opened the way to discovering the molecular basis of development in actinomycetes. Desferrioxamine (DFO) production and import of ferrioxamines (FO; iron-complexed DFO) are key to triggering morphogenesis of S. coelicolor and we show here that growth of S. coelicolor on the reference medium for Streptomyces developmental studies is fully dependent on DFO biosynthesis. UPLC-ESI-MS analysis revealed that all bld mutants tested are affected in DFO biosynthesis, with bldA, bldJ, and ptsH mutants severely impaired in DFO production, while bldF, bldK, crr and ptsI mutants overproduce DFO.Morphogenesis of bldK and bldJ mutants was recovered by supplying exogenous iron. Transcript analysis showed that the bldJ mutant is impaired in expression of genes involved in the uptake of FO, whereas transcription of genes involved in both DFO biosynthesis and FO uptake is increased in bldK mutants. Our study allows proposing altered DFO production and/or FO uptake as a novel phenotypic marker of many S. coelicolor bld mutants, and strengthens the role of siderophores and iron acquisition in morphological development of actinomycetes.

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Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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“…The use of cyclic AMP (cAMP), N-butyryl homoserine lactone, or N-oxohexanoyl-DL-homoserine lactone at a low concentration of 10 M permitted an increase in the microbial recovery of heterotrophic bacteria from the central Baltic Sea (146). The concept of mutualism between bacterial species emerged and was significantly highlighted by the role of siderophores (147)(148)(149). D'Onofrio et al identified 5 new acyldesferrioxamine siderophores isolated from Micrococcus luteus KLE1011, permitting the culture of a substantial number of marine bacterial species previously considered to be uncultivable (147).…”

Section: Signaling Compoundsmentioning

confidence: 99%

The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota

et al. 2015

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SUMMARY Bacterial culture was the first method used to describe the human microbiota, but this method is considered outdated by many researchers. Metagenomics studies have since been applied to clinical microbiology; however, a “dark matter” of prokaryotes, which corresponds to a hole in our knowledge and includes minority bacterial populations, is not elucidated by these studies. By replicating the natural environment, environmental microbiologists were the first to reduce the “great plate count anomaly,” which corresponds to the difference between microscopic and culture counts. The revolution in bacterial identification also allowed rapid progress. 16S rRNA bacterial identification allowed the accurate identification of new species. Mass spectrometry allowed the high-throughput identification of rare species and the detection of new species. By using these methods and by increasing the number of culture conditions, culturomics allowed the extension of the known human gut repertoire to levels equivalent to those of pyrosequencing. Finally, taxonogenomics strategies became an emerging method for describing new species, associating the genome sequence of the bacteria systematically. We provide a comprehensive review on these topics, demonstrating that both empirical and hypothesis-driven approaches will enable a rapid increase in the identification of the human prokaryote repertoire.

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“…Furthermore, we performed screening for bacterial isolates with a desferrioxaminedependent phenotype and observed a stricter dependence of growth on the supply of desferrioxamine with regard to the isolates affiliated with Microbacterium. 25 In an isolate of Janthinobacterium lividum, treatment with desferrioxamine does not strongly affect the growth but stimulates the production of violacein, a purple pigment antibiotic. In contrast to the positive effect, growth of some bacteria is significantly inhibited due to the presence of desferrioxamine.…”

Section: Desferrioxaminesmentioning

confidence: 99%

“…In contrast to the positive effect, growth of some bacteria is significantly inhibited due to the presence of desferrioxamine. 25 This finding suggests that those bacteria do not retain the ability to use ferrioxamine but have a different ferric-ion uptake mechanism.…”

Section: Desferrioxaminesmentioning

confidence: 99%

Antibiotics in microbial coculture

Uéda

Beppu

2016

J Antibiot

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Today, the frequency of discovery of new antibiotics in microbial culture is significantly decreasing. The evidence from whole-genome surveys suggests that many genes involved in the synthesis of unknown metabolites do exist but are not expressed under conventional cultivation conditions. Therefore, it is urgently necessary to study the conditions that make otherwise silent genes active in microbes. Here we overview the knowledge on the antibiotic production promoted by cocultivation of multiple microbial strains. Accumulating evidence indicates that cocultivation can be an effective way to stimulate the production of substances that are not formed during pure cultivation. Characterization of the promotive factors produced by stimulator strains is expected to give clues to the development of effective cultivation conditions for drug discovery. The Journal of Antibiotics (2017) 70, 361-365; doi:10.1038/ja.2016; published online 19 October 2016 INTRODUCTIONEver since the discovery of penicillin, antibiotics and many other kinds of biologically active substances have been obtained from microbial cultures. The divergent actions of these compounds have contributed to the current knowledge of the basic mechanisms of molecular interaction and therapeutic methods as well. This special issue contains studies dealing with the milestone works that have advanced the development of basic biological sciences and medicinal applications.In contrast to the great successes in mining of useful natural products in the last century, the frequency of isolation of new compounds from microbial cultures is substantially decreasing in this century. 1 In accordance with this situation, the pharmaceutical industry is changing the direction of drug development and reducing the weight of natural products among the resources for screening.The factors limiting the chances of new discovery can be attributed to humans, not to microorganisms. Studies on the accumulating genomic information indicate that a large number of biosynthetic gene clusters are not expressed under the conventional culture conditions. 1,2 Namely, the potential diversity of microbial products is still large, but scientists do not yet know how to fully take advantage of the abilities of microbes. This problem is due in part to the fact that the methods of modern microbiology still depend on the manipulation techniques developed by Robert Koch and colleagues, which relies on the single-colony isolation from solid agar plates and subsequent pure cultivation under laboratory conditions. We can imagine that the dependence on those artificial conditions restricts access to a number of microorganisms and/or their abilities.To address the issue, attempts have been made to discover new substances specifically produced in coculture. In natural environments, microbes are living in diverse associations with other organisms. The modes of interaction include antagonism, commensalism and mutualism. Functions specifically correlating with such interactions may not get activated during conv...

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Divergent effects of desferrioxamine on bacterial growth and characteristics

Cited by 21 publications

References 10 publications

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota

Antibiotics in microbial coculture

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