Divergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (SERM) in the teleost Sparus auratus

Vieira, Florbela A.; Pinto, Patrícia I. S.; Guerreiro, Pedro M.; Power, Deborah M.

doi:10.1016/j.ygcen.2012.09.018

Cited by 19 publications

(10 citation statements)

References 43 publications

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“…Primer sequences, amplicon size, amplicon melting temperature, reaction efficiency, R 2 and the accession number of genes are listed in Table 2. Standard curves relating amplification cycle to initial template quantity (in copy number, calculated as in Vieira et al, 2012) were generated using serial dilutions of purified and quantified target amplicons. All amplicons were sequenced to confirm qPCR specificity.…”

Section: Analysis Of Gene Expression By Quantitative Real-time Pcr (Qmentioning

confidence: 99%

Thermal imprinting modifies bone homeostasis in cold challenged sea bream (Sparus aurata, L.)

Mateus

Costa

Gisbert

et al. 2017

Journal of Experimental Biology

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Fish are ectotherms and temperature plays a determinant role in their physiology, biology and ecology, and is a driver of seasonal responses. The present study assessed how thermal imprinting during embryonic and larval stages modified the response of adult fish to low water temperature. We targeted the gilthead sea bream, which develops a condition known as winter syndrome when it is exposed to low water temperatures. Eggs and larvae of sea bream were exposed to four different thermal regimes and then the response of the resulting adults to a low temperature challenge was assessed. Sea bream exposed to a high-low thermal regime as eggs and larvae (HLT; 22°C until hatch and then 18°C until larvae-juvenile transition) had increased plasma cortisol and lower sodium and potassium in response to a cold challenge compared with the other thermal history groups. Plasma glucose and osmolality were increased in coldchallenged HLT fish relative to the unchallenged HLT fish. Cold challenge modified bone homeostasis/responsiveness in the lowhigh thermal regime group (LHT) relative to other groups, and ocn, ogn1/2, igf1, gr and trα/β transcripts were all downregulated. In the low temperature group (LT) and HLT group challenged with a low temperature, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) activities were decreased relative to unchallenged groups, and bone calcium content also decreased in the LT group. Overall, the results indicate that thermal imprinting during early development of sea bream causes a change in the physiological response of adults to a cold challenge.

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Section: Analysis Of Gene Expression By Quantitative Real-time Pcr (Qmentioning

confidence: 99%

Thermal imprinting modifies bone homeostasis in cold challenged sea bream (Sparus aurata, L.)

Mateus

Costa

Gisbert

et al. 2017

Journal of Experimental Biology

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“…Transcripts were quantified using the relative standard curve method (Vieira et al 2012a), generated using serial dilutions of specific qPCR products for each gene (obtained using the same species, tissues and primers for qPCR analysis). All amplicons were sequenced to confirm their identity.…”

Section: :3mentioning

confidence: 99%

Thermal imprinting modifies adult stress and innate immune responsiveness in the teleost sea bream

Mateus

Costa

Cardoso

et al. 2017

Journal of Endocrinology

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The impact of thermal imprinting on the plasticity of the hypothalamic-pituitary-interrenal (HPI) axis and stress response in an adult ectotherm, the gilthead sea bream (, L.), during its development was assessed. Fish were reared under 4 thermal regimes, and the resulting adults exposed to acute confinement stress and plasma cortisol levels and genes of the HPI axis were monitored. Changes in immune function, a common result of stress, were also evaluated using histomorphometric measurements of melanomacrophages centers (MMCs) in the head kidney and by monitoring macrophage-related transcripts. Thermal history significantly modified the HPI responsiveness in adult sea bream when eggs and larvae were reared at a higher than optimal temperature (HT, 22°C), and they had a reduced amplitude in their cortisol response and significantly upregulated pituitary and head kidney transcripts. Additionally, after an acute stress challenge, immune function was modified and the head kidney of adult fish reared during development at high temperatures (HT and LHT, 18-22°C) had a decreased number of MMCs and a significant downregulation of dopachrome tautomerase. Thermal imprinting during development influenced adult sea bream physiology and increased plasma levels of glucose and sodium even in the absence of an acute stress in fish reared under a high-low thermal regime (HLT, 22-18°C). Overall, the results demonstrate that temperature during early development influences the adult HPI axis and immune function in a teleost fish.

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“…In addition, the selective ER modulator (SERM), raloxifene (an ER agonist in mammalian bone), upregulated both ERβa and βb in dentary bone and downregulated ERβa in vertebra [72]. Taken together, these studies suggest that estrogenic compounds most probably exert at least part of their actions on the fish skeleton or skin-scale through the duplicate ERβs (especially the βa isoform), rather than ERα.…”

Section: Mechanisms Of Estrogenic Action In Mineralized Tissuesmentioning

confidence: 99%

“…In common with mammals, it appears that the expression, localization and regulation of different ER isoform in fish differ between different types of mineralized tissues [42,46,72] (Table 1), probably giving rise to different responsiveness to estrogens and to the potential for interaction or different actions between ER isoforms. The relative and tissue-specific role of ER isoforms in mediating estrogenic actions in fish mineralized tissues, as well as the expression and involvement of other possible players (e.g., ER variants, membrane ERs, other hormones or their receptors) will need further investigation.…”

Section: Mechanisms Of Estrogenic Action In Mineralized Tissuesmentioning

confidence: 99%

“…For example, scales are proposed to be a preferential site for E 2 -induced Ca mobilization compared to bone [33,39], and they are directly exposed to the aquatic environment and for these reasons have been preferentially studied as an EDC target tissue (Table 1). However, the estrogenic EDCs impacts on the fish endoskeleton and different bone types (endochondral, dermal, chondroid) need to be studied, as they respond differently to estrogenic compounds [46,72];The number of contaminants present in the aquatic environment both alone (in different concentrations) or in complex mixtures that may have an estrogenic disrupting action. …”

Section: Estrogenic Endocrine Disruption In Mineralized Tissuesmentioning

confidence: 99%

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Effects of Estrogens and Estrogenic Disrupting Compounds on Fish Mineralized Tissues

2014

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Estrogens play well-recognized roles in reproduction across vertebrates, but also intervene in a wide range of other physiological processes, including mineral homeostasis. Classical actions are triggered when estrogens bind and activate intracellular estrogen receptors (ERs), regulating the transcription of responsive genes, but rapid non-genomic actions initiated by binding to plasma membrane receptors were recently described. A wide range of structurally diverse compounds from natural and anthropogenic sources have been shown to interact with and disrupt the normal functions of the estrogen system, and fish are particularly vulnerable to endocrine disruption, as these compounds are frequently discharged or run-off into waterways. The effect of estrogen disruptors in fish has mainly been assessed in relation to reproductive endpoints, and relatively little attention has been given to other disruptive actions. This review will overview the actions of estrogens in fish, including ER isoforms, their expression, structure and mechanisms of action. The estrogen functions will be considered in relation to mineral homeostasis and actions on mineralized tissues. The impact of estrogenic endocrine disrupting compounds on fish mineralized tissues will be reviewed, and the potential adverse outcomes of exposure to such compounds will be discussed. Current lacunae in knowledge are highlighted along with future research priorities.

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Divergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (SERM) in the teleost Sparus auratus

Cited by 19 publications

References 43 publications

Thermal imprinting modifies bone homeostasis in cold challenged sea bream (Sparus aurata, L.)

Thermal imprinting modifies bone homeostasis in cold challenged sea bream (Sparus aurata, L.)

Thermal imprinting modifies adult stress and innate immune responsiveness in the teleost sea bream

Effects of Estrogens and Estrogenic Disrupting Compounds on Fish Mineralized Tissues

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