Divergent structures and functions of the Cupin proteins in plants

Hu, Fei; Ye, Ziyi; Dong, Kui; Zhang, Weimeng; Fang, Dongdong; Cao, Jun

doi:10.1016/j.ijbiomac.2023.124791

Cited by 6 publications

(1 citation statement)

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“…Germins and germin-like proteins (GLPs) belong to the cupin superfamily of plant proteins and play an important role in the plant’s defensive mechanism against osmotic and homeostasis stresses . Additionally, they are involved in enhancing the plant’s resistance to oxidative stress caused by both abiotic and biotic stimuli, − have provided a comprehensive description of the germins and GLPs, highlighting their occurrence in plants and their association with several developmental processes. , Additionally, it has been reported that the genome of O. sativa has a considerable collection of GLPs.…”

Section: Introductionmentioning

confidence: 99%

Genomic Exploration: Unraveling the Intricacies of Indica Rice Oryza sativa L. Germin-Like Protein Gene 12-3 (OsGLP12-3) Promoter via Cloning, Sequencing, and In Silico Analysis

Ahmad,

Ullah,

Ullah

et al. 2024

ACS Omega

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Germin and Germin-like proteins (GLPs) are a class of plant proteins that are part of the Cupins superfamily, found in several plant organs including roots, seeds, leaves, and nectar glands. They play a crucial role in plant defense against pathogens and environmental stresses. Herein, this study focused on the promoter analysis of OsGLP12-3 in rice cultivar Swat-1 to elucidate its regulation and functions. The region (1863bp) of the OsGLP12-3 promoter from Swat-1 genomic DNA was amplified, purified, quantified, and cloned using Topo cloning technology, followed by sequencing. Further in silico comparative analysis was conducted between the OsGLP12-3 promoters from Nipponbare and Swat-1 using the Plant CARE database, identifying 24 cis-acting regulatory elements with diverse functions. These elements exhibited distinct distribution patterns in the 2 rice varieties. The OsGLP12-3 promoter revealed an abundance of regulatory elements associated with biotic and abiotic stress responses. Computational tools were employed to analyze the regulatory features of this region. In silico expression analysis of OsGLP12-3, considering various developmental stages, stress conditions, hormones, and expression timing, was performed using the TENOR tool. Pairwise alignment indicated 86% sequence similarity between Nipponbare and Swat-1. Phylogenetic analysis was conducted to explore the evolutionary relationship between the OsGLP12-3 and other plant GLPs. Additionally, 2 unique regulatory elements were modeled and docked, GARE and MBS to understand their hydrogen bonding interactions in gene regulation. The study highlights the importance of OsGLP12-3 in plant defense against biotic and abiotic stresses, supported by its expression patterns in response to various stressors and the presence of specific regulatory elements within its promoter region.

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