Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species

Abstract

This study investigated the biochemical properties, enzyme activities, isoenzyme pattern, and molecular weight of three types of digestive enzyme from six freshwater fish species: Puntius gonionotus (common silver barb), Puntioplites proctozysron (Smith’s barb), Oreochromis niloticus (Nile tilapia), Hemibagrus spilopterus (yellow mystus), Ompok bimaculatus (butter catfish), and Kryptopterus geminus (sheatfish). The optimum pHs for amylase and alkaline protease activities were 7.0–8.0 and 8.0–10.0, and the optimum temperatures were 45–60 °C and 50–55 °C, respectively. A pepsin-like enzyme was detected in all three carnivorous fishes (Ompok bimaculatus, Kryptopterus geminus, and Hemibagrus spilopterus) with optimum reaction pH of 2.0 for each and optimum reaction temperatures 50–55 °C. In optimum reaction conditions, the amylase and alkaline protease from Puntioplites proctozyron showed the highest activities. Lower activities of all enzymes were observed at temperature (29 °C) of Lam Nam Choen swamp than at the optimum reaction temperatures. The fish species contained one to three and five to eight isoforms of amylase and alkaline protease, respectively, with molecular weights from 19.5 to 175 kDa. Both the alkaline proteases and amylases were stable in wide pH and temperature ranges.

Studies on the feeding habit and digestive enzyme activities in three small indigenous fish species from Assam, India

“…In the current study, high protease activity was observed in M. tengara compared to others. Similarly lower activity of amylase and higher alkaline protease activities in carnivorous fish than in the omnivorous and herbivorous fish species was reported by Champasri et al (2021). High proteases and trypsin activities observed in M. tengara in the present study are in agreement with findings in other carnivorous fish species (Gioda et al 2017;Weinrauch et al 2019).…”

Thermal stress response of different age group of Nile tilapia Oreochromis niloticus (Linnaeus, 1758) exposed to various temperature regimes

“…This DSI value is similar to low when compared to other in freshwater sh viz. Puntioplites proctozysron, Hemibagrus spilopterus, Ompok bimaculatus, and Kryptopterus geminus but lower than Puntius gonionotus, Oreochromis niloticus, (Champasri et al 2021). In the present study, no signi cant differences in the DSI of adult tilapia were observed in three temperatures (28°C, 30°C and 32°C).…”

Multipotential Alkaline Protease From a Novel Pyxidicoccus sp. 252: Ecofriendly Replacement to Various Chemical Processes

“…The activity and stability of PyCP in the alkaline pH range makes it suitable as an additive in detergent formulations and in dehairing of the skin in the leather industry ( Abdel Fattah et al, 2016 ; Suginta et al, 2016 ; Champasri et al, 2021 ; Li et al, 2021 ). The pH optimum of commercial detergent proteases (Savinase, Subtilisin Carlsberg, and Subtilisin Novo) is pH 10.5, and they are stable in the pH range 7.0–12.0 ( Niyonzima and More, 2015 ).…”