2003
DOI: 10.1089/104454903322624993
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Diverse Effects of Nanosecond Pulsed Electric Fields on Cells and Tissues

Abstract: The application of pulsed electric fields to cells is extended to include nonthermal pulses with shorter durations (10-300 ns), higher electric fields (< or =350 kV/cm), higher power (gigawatts), and distinct effects (nsPEF) compared to classical electroporation. Here we define effects and explore potential application for nsPEF in biology and medicine. As the pulse duration is decreased below the plasma membrane charging time constant, plasma membrane effects decrease and intracellular effects predominate. Ns… Show more

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Cited by 234 publications
(186 citation statements)
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“…Different works have reported about the effects on animal cells of these kinds of pulses, commonly named nanosecond pulsed electric fields (nsPEF). More precisely, it has been shown that the gene electrotransfection efficiency can be improved by nsPEF exposure, also called nanoporation, (Beebe et al 2003) and that disturbances on the cell membrane could be sufficient to render it permeable to small molecules, such as propidium iodide (Ibey et al 2009;Vernier et al 2006). Beyond their potential effects on the cell membrane, these nsPEF show a great interest because they also offer the possibility to disturb the intra cellular structures and functions (Beebe and Schoenbach 2005).…”
Section: Introductionmentioning
confidence: 99%
“…Different works have reported about the effects on animal cells of these kinds of pulses, commonly named nanosecond pulsed electric fields (nsPEF). More precisely, it has been shown that the gene electrotransfection efficiency can be improved by nsPEF exposure, also called nanoporation, (Beebe et al 2003) and that disturbances on the cell membrane could be sufficient to render it permeable to small molecules, such as propidium iodide (Ibey et al 2009;Vernier et al 2006). Beyond their potential effects on the cell membrane, these nsPEF show a great interest because they also offer the possibility to disturb the intra cellular structures and functions (Beebe and Schoenbach 2005).…”
Section: Introductionmentioning
confidence: 99%
“…They provide a new approach for physically targeting intracellular organelles with many applications, including the initiation of apoptosis in cultured cells [12][13][14] and tumors [6], enhancement of gene transfection efficiency [13,14], and inhibiting tumor growth [6]. During the past year, we have treated over 300 murine melanomas in 120 mice with 40 kV/cm electric field pulses 300 ns in duration with dramatic results.…”
mentioning
confidence: 99%
“…Applying pulses with higher field strength (50-300 kV/cm) and shorter duration (10-300 ns) do not fully charge the cell membrane and instead interact primarily with the membranes of intracellular organelles [4]. These nanosecond duration PEFs (nsPEFs) cause intracellular calcium release [5,6] and apoptosis-induction in cell suspensions and in vivo tumors [5,7,8]. To better understand the mechanisms involved, attempts to develop mathematical models are underway 0006-291X/$ -see front matter Ó 2007 Elsevier Inc. All rights reserved.…”
mentioning
confidence: 99%