Diverse forms of Pin-II family proteinase inhibitors from Capsicum annuum adversely affect the growth and development of Helicoverpa armigera

Tamhane, Vaijayanti A.; Giri, Ashok P.; Sainani, Mohini N.; Gupta, Vidya S.

doi:10.1016/j.gene.2007.07.024

Cited by 56 publications

(38 citation statements)

References 37 publications

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“…A characteristic feature of PI-II proteins is that they contain one to several sequence repeats, which code for one precursor. Proteolytic cleavage of these precursors at specific linker sites results in several single or multidomain PIs with different properties (Atkinson et al, 1993;Beekwilder et al, 2000;Horn et al, 2005;Tamhane et al, 2007), which could explain the absence of more than two bands when silencing genes encoding the two-domain proteins SPI2a and SPI2b. It is possible that other peptides with low activity or concentration were not detected or resolved with the method that we used and that there are in fact more than three SPI peptides missing in the (A) Mean 6 SE relative transcript abundance of SPI1, SPI2a, and SPI2b in leaves of wild-type plants and of transgenic lines expressing an irconstruct specific for SPI2a and SPI2b (irSPI2a+b) or a construct specific for SPI1, SPI2a, and SPI2b (irSPI1/2a+b) (n = 7).…”

Section: Spi2a and Spi2b Are Strong Inhibitors Of Subtilisin But Accomentioning

confidence: 99%

Serine Protease Inhibitors Specifically Defend Solanum nigrum against Generalist Herbivores but Do Not Influence Plant Growth and Development

et al. 2010

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Solanaceaeous taxa produce diverse peptide serine proteinase inhibitors (SPIs), known antidigestive defenses that might also control endogenous plant proteases. If and how a plant coordinates and combines its different SPIs for the defense against herbivores and if these SPIs simultaneously serve developmental functions is unknown. We examine Solanum nigrum's SPI profile, comprising four different active inhibitors, of which the most abundant proved to be novel, to understand their functional specialization in an ecological context. Transcript and activity characterization revealed tissuespecific and insect-elicited accumulation patterns. Stable and transient gene silencing of all four SPIs revealed different specificities for target proteinases: the novel SPI2c displayed high specificity for trypsin and chymotrypsin, while two other SPI2 homologs were highly active against subtilisin. In field and lab experiments, we found all four SPIs to display herbivoreand gene-specific defensive properties, with dissimilar effects on closely related species. However, we did not observe any clear developmental phenotype in SPI-silenced plants, suggesting that SPIs do not play a major role in regulating endogenous proteases under the conditions studied. In summary, specific single SPIs or their combinations defend S. nigrum against generalist herbivores, while the defense against herbivores specialized on SPI-rich diets requires other unknown defense mechanisms.

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Section: Spi2a and Spi2b Are Strong Inhibitors Of Subtilisin But Accomentioning

confidence: 99%

Serine Protease Inhibitors Specifically Defend Solanum nigrum against Generalist Herbivores but Do Not Influence Plant Growth and Development

et al. 2010

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“…Another important example of molecular co-evolution of plants and insects is the interplay of insect gut proteases and their proteinaceous inhibitors expressed by plants. Proteinase inhibitors expressed in plant tissues retard the growth and development of insects by disturbing their digestive metabolism (Green and Ryan 1972;Tamhane et al 2005Tamhane et al , 2007. In terms of indirect defense mechanisms many plant species develop extrafloral nectaries in order to attract natural enemies of herbivores such as ants (Oliveira and Freitas 2004).…”

Section: Diversification For Fitness and Survival: Coevolution Of Traitsmentioning

confidence: 99%

Ecological turmoil in evolutionary dynamics of plant–insect interactions: defense to offence

Mishra

Lomate

Joshi

et al. 2015

Planta

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Available history manifests contemporary diversity that exists in plant-insect interactions. A radical thinking is necessary for developing strategies that can co-opt natural insect-plant mutualism, ecology and environmental safety for crop protection since current agricultural practices can reduce species richness and evenness. The global environmental changes, such as increased temperature, CO₂ and ozone levels, biological invasions, land-use change and habitat fragmentation together play a significant role in re-shaping the plant-insect multi-trophic interactions. Diverse natural products need to be studied and explored for their biological functions as insect pest control agents. In order to assure the success of an integrated pest management strategy, human activities need to be harmonized to minimize the global climate changes. Plant-insect interaction is one of the most primitive and co-evolved associations, often influenced by surrounding changes. In this review, we account the persistence and evolution of plant-insect interactions, with particular focus on the effect of climate change and human interference on these interactions. Plants and insects have been maintaining their existence through a mutual service-resource relationship while defending themselves. We provide a comprehensive catalog of various defense strategies employed by the plants and/or insects. Furthermore, several important factors such as accelerated diversification, imbalance in the mutualism, and chemical arms race between plants and insects as indirect consequences of human practices are highlighted. Inappropriate implementation of several modern agricultural practices has resulted in (i) endangered mutualisms, (ii) pest status and resistance in insects and (iii) ecological instability. Moreover, altered environmental conditions eventually triggered the resetting of plant-insect interactions. Hence, multitrophic approaches that can harmonize human activities and minimize their interference in native plant-insect interactions are needed to maintain natural balance between the existence of plants and insects.

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“…Protease inhibitors when incorporated into artificial diets or expressed in transgenic plants increase mortality [174] and reduce the growth and development of larvae from many insect pest species including Coleoptera [175,176], Orthoptera [177] and Lepidoptera [178,179] (Table 2). The mechanisms by which ingested PIs mediate their effects on insect physiology differs between insect species [180].…”

Section: Mechanism Of Action Of Protease Inhibitors On Lepidopteran Imentioning

confidence: 99%

Biotechnological Approaches for the Control of Insect Pests in Crop Plants

Stevens¹,

Dunse²,

Fox³

et al. 2012

Pesticides - Advances in Chemical and Botanical Pesticides

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Diverse forms of Pin-II family proteinase inhibitors from Capsicum annuum adversely affect the growth and development of Helicoverpa armigera

Cited by 56 publications

References 37 publications

Serine Protease Inhibitors Specifically Defend Solanum nigrum against Generalist Herbivores but Do Not Influence Plant Growth and Development

Serine Protease Inhibitors Specifically Defend Solanum nigrum against Generalist Herbivores but Do Not Influence Plant Growth and Development

Ecological turmoil in evolutionary dynamics of plant–insect interactions: defense to offence

Biotechnological Approaches for the Control of Insect Pests in Crop Plants

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