Diverse mechanisms of osteoblast spreading on hydroxyapatite and titanium

Mizutani, Takashi; Hosokawa, Ryuji; Okamoto, Keiko; Kimoto, T.; Akagawa, Yasumasa

doi:10.1016/s0142-9612(99)00264-1

Cited by 118 publications

(91 citation statements)

References 31 publications

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“…The composite showed a viscosity level 36 dPa.s. The viscocity values possessed by this composite is very close to the standard values of injectable bone substitute which is 40 dPa.s 26 . Qualitatively, the suspension made from the composite can be applied as an injectable bone substitute.…”

Section: Resultssupporting

confidence: 72%

SYNTHESIS OF HAp-CHITOSAN-PVA COMPOSITE AS INJECTABLE BONE SUBSTITUTE MATERIAL

2017

Rasayan J Chem

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The main objective of this study is to synthesize a composite of hydroxyapatite (HAp)-chitosan-polyvinyl alcohol (PVA) and to evaluate its uses as bone filler. An injectable bone substitute (IBS) material is a suspension of bone filler which applied by injection to fill the gaps caused by osteoporosis. A hydroxyapatite used as the composite's component was synthesized from Tutut shell. The composite was made by mixing hydroxyapatite, chitosan and PVA to homogeneous followed by irradiation with gamma rays at 20 kGy. The composite was characterized by FTIR analysis, XRD, SEM, viscosity test, and cytotoxicity assay. The FTIR analysis showed the bond formation between hydroxyapatite and chitosan, which later formed cross linkages by the addition of PVA. The viscosity of the composite was 36dPa. The cytotoxicity assay (MTT assay) showed that the suspension is not toxic because the cell viability value was obtained at more than 50%. As a conclusion, the composite of HAp from tutut shell-chitosan-PVA is suitable to be used as an injectable bone substitute.

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Section: Resultssupporting

confidence: 72%

SYNTHESIS OF HAp-CHITOSAN-PVA COMPOSITE AS INJECTABLE BONE SUBSTITUTE MATERIAL

2017

Rasayan J Chem

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“…In this context, the aspects of cell adhesion, cell morphology, cell surface receptors, and cytokine production have all been well studied 6,[18][19][20][21] . However, the molecular events that take place at the interface between bone and dental implants particularly the role of osteoclasts during osseointegration, long-term bone remodeling, and in implant-related diseases are still unknown.…”

Section: Discussionmentioning

confidence: 99%

Titanium Surface Roughness Accelerates RANKL-dependent Differentiation in the Osteoclast Precursor Cell Line, RAW264.7

et al. 2007

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The present study was a molecular analysis of the initial differentiation of osteoclast precursor RAW264.7 cells on titanium specimens. RAW264.7 cell line was cultured on titanium specimens of which the surfaces were finished by wet grinding with 2000-, 1200-, 600-, or 180-grit waterproof abrasive paper. Total RNA was extracted from cells cultured in the presence or absence of Receptor Activator of NF-кB Ligand (RANKL), prior to cDNA synthesis for real-time quantitative reverse transcriptase-polymerase chain reaction analysis.Titanium surfaces initially enhanced the expression of osteoclast differentiation markers including tartrate-resistant acid phosphatase and cathepsin K in RAW264.7 cells cultured with RANKL stimulation, in a roughness-dependent manner. The mRNA expressions of both RANKL receptor, RANK, and its adapter protein TNF receptor-associated factor 6 (TRAF6) increased when RAW264.7 cells were cultured on titanium specimens with roughened surfaces, as compared with that of control specimen with a polished surface. These results, taken together, suggested that titanium surface roughness facilitated osteoclast differentiation through the activation of the RANK-TRAF6 signaling network.

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“…It was found that osteoblasts were able to adhere to titanium through production of ECM proteins that was absorbed onto the surface of titanium (Matsuura et al, 2000). The quantities of ECM proteins produced were, however, insufficient while some proteins were not produced at all, leading to inadequate bone anchorage.…”

Section: Interaction Of Biomaterials With Cellsmentioning

confidence: 99%

Reprogramming and Carcinogenesis—Parallels and Distinctions

Wasik

Grabarek

Pantovic

et al. 2014

International Review of Cell and Molecular Biology

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Abbreviations: CDK -Cyclin-Dependent Kinase; CSC -cancer stem cells; DSB -double strand breaks ; ECM -extracellular matrix; ES cell -embryonic stem cell; GFP -green fluorescent protein; GSK3 -glycogen synthase kinase-3; HN -Hemagglutininneuraminidase; iPS -induced pluripotent stem cells; MSFs -myocardial scar fibroblasts; RB -Retinoblastoma protein; RGD -arginine-glycine-aspartic acid; RISC -RNA-induced silencing complex; Shc -Src homology 2 domain-containing.

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Diverse mechanisms of osteoblast spreading on hydroxyapatite and titanium

Cited by 118 publications

References 31 publications

SYNTHESIS OF HAp-CHITOSAN-PVA COMPOSITE AS INJECTABLE BONE SUBSTITUTE MATERIAL

SYNTHESIS OF HAp-CHITOSAN-PVA COMPOSITE AS INJECTABLE BONE SUBSTITUTE MATERIAL

Titanium Surface Roughness Accelerates RANKL-dependent Differentiation in the Osteoclast Precursor Cell Line, RAW264.7

Reprogramming and Carcinogenesis—Parallels and Distinctions

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