2018
DOI: 10.1186/s12862-018-1274-2
|View full text |Cite
|
Sign up to set email alerts
|

Diverse RNA interference strategies in early-branching metazoans

Abstract: BackgroundMicro RNAs (miRNAs) and piwi interacting RNAs (piRNAs), along with the more ancient eukaryotic endogenous small interfering RNAs (endo-siRNAs) constitute the principal components of the RNA interference (RNAi) repertoire of most animals. RNAi in non-bilaterians – sponges, ctenophores, placozoans and cnidarians - appears to be more diverse than that of bilaterians, and includes structurally variable miRNAs in sponges, an enormous number of piRNAs in cnidarians and the absence of miRNAs in ctenophores … Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

3
19
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
6
1
1

Relationship

0
8

Authors

Journals

citations
Cited by 22 publications
(22 citation statements)
references
References 87 publications
3
19
0
Order By: Relevance
“…However, upon close inspection, the biogenesis and function of the endogenous small RNAs in whitefly are quite different. Our work reinforces the consistent observation that non-miRNA RNAi pathways are fluid; clade-specific duplication of the RNAi factors is common, even loss of an entire class of small RNA has occurred in several metazoan clades (Sarkies et al, 2015;Calcino et al, 2018;Mondal et al, 2018). Furthermore, these findings illustrate the benefits of in-depth dissection of the RNAi biology for evolutionarily and biologically different organisms, beyond those examined in model study systems, for developing genetic technology.…”
Section: Discussionsupporting
confidence: 84%
“…However, upon close inspection, the biogenesis and function of the endogenous small RNAs in whitefly are quite different. Our work reinforces the consistent observation that non-miRNA RNAi pathways are fluid; clade-specific duplication of the RNAi factors is common, even loss of an entire class of small RNA has occurred in several metazoan clades (Sarkies et al, 2015;Calcino et al, 2018;Mondal et al, 2018). Furthermore, these findings illustrate the benefits of in-depth dissection of the RNAi biology for evolutionarily and biologically different organisms, beyond those examined in model study systems, for developing genetic technology.…”
Section: Discussionsupporting
confidence: 84%
“…These results confirmed our hypothesis as NveAGO2 but not NveAGO1 is enriched with endo-siRNAs throughout development. To further confirm this hypothesis, we quantified endo-siRNAs that were annotated independently in a previous study 32 . Although this list was not generated by AGO-IP sequencing, these annotated endo-siRNA were enriched in our AGO2-IP data over AGO1-IP and the negative control (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 90%
“…Our initial expectation was to identify a similar type of specialization in Hexacorallia as it is known that endogenous siRNAs (endo-siRNAs) are present in Nematostella 9 , 32 . Using ShortStack 33 , 34 we identified endo-siRNAs in our libraries (Supplementary Data 4 ) and quantified their normalized read counts in NveAGO1 and NveAGO2 IP from three developmental stages (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The siRNA pathway is considered the most ancient and at the origin of the RNAi phenomenon. Its main components (Argonaute-Piwi, Dicer-like, and RNA-dependent RNA Polymerase proteins) are supposed to have been already present in the last common ancestor of eukaryotes (7).…”
Section: Introductionmentioning
confidence: 99%
“…In Drosophila melanogaster the siRNA pathway acts as the main antiviral immune response (8) and is also involved in somatic defense against transposons (9). However, the fact that several siRNAs target cellular mRNAs (7,10) and repress the expression of specific genes (11), suggests that it may also play a role in the regulation of gene expression.…”
Section: Introductionmentioning
confidence: 99%