Diverse serologic response to rotavirus infection of infants in a single epidemic

Hf, Clark; Kt, Dolan; Horton-Slight, Paula; Palmer, J.; Sa, Plotkin

doi:10.1097/00006454-198511000-00006

Cited by 41 publications

(25 citation statements)

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“…The fourth child developed a possible heterotypic antibody response. Heterotypic immunity in man has also been reported by Clark et al (1985). It appears from the immunoprecipitation data that the possible heterotypic immunity might be mediated by antibody to VP3, since there is no response to VP7 as seen by RIPA.…”

Section: Discussionmentioning

confidence: 57%

“…Previous studies analysing the immune response in humans to different polypeptides have exclusively used antigen not derived from naturally infecting virus (Clark et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, human sera have been shown to immunoprecipitate certain structural polypeptides of bovine rotavirus in a study using purified virus, labelled in vitro, as antigen (Clark et al, 1985). The aim of the present study was to analyse the human immune response to both structural and non-structural polypeptides of naturally infecting human rotavirus.…”

confidence: 99%

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Serum Antibody Responses to Individual Viral Polypeptides in Human Rotavirus Infections

Svensson¹,

Sheshberadaran²,

Vene³

et al. 1987

Journal of General Virology

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SUMMARYA radioimmunoprecipitation assay (RIPA) was used to study the serum antibody responses to individual polypeptides that developed after infection with viruses from human rotavirus subgroups I and II. Paired sera from eight children (1 to 8.5 years of age) were used in the study. Although all of the eight acute sera were negative by the complement fixation test, four of them were positive by RIPA, indicating a previous infection by rotavirus. A significant difference in the number of polypeptides immunoprecipitated was seen among the convalescent sera. The number of polypeptides immunoprecipitated was found to be related to previous infection experience. At most, ten different polypeptides were immunoprecipitated: seven structural polypeptides VPl to VP7 and three non-structural polypeptides, NSl, NS 2 and NS3. No sera immunoprecipitated VP8 or VP9. Acute sera positive by RIPA immunoprecipitated up to five polypeptides, VP1, VP2, VP3, VP4 and VP6. One of the non-structural proteins (NS2) was found to be particularly immunogenic, since antibodies to this polypeptide were detected in several convalescent sera. Among the structural proteins VP2 and VP6 were found to be the two immunodominant polypeptides which were recognized by all convalescent sera. Only three convalescent sera immunoprecipitated VP7, the major type-specific antigen responsible for inducing neutralizing antibodies. Three of four originally seronegative children with no reactivity in the convalescent sera to VP7 developed neutralizing antibodies to a single serotype. One child developed antibodies to two serotypes.

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Section: Discussionmentioning

confidence: 57%

“…Previous studies analysing the immune response in humans to different polypeptides have exclusively used antigen not derived from naturally infecting virus (Clark et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

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Serum Antibody Responses to Individual Viral Polypeptides in Human Rotavirus Infections

Svensson¹,

Sheshberadaran²,

Vene³

et al. 1987

Journal of General Virology

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“…Studies of monkeys have demonstrated that the passive transfer of serum antibodies can provide protection against infection (89). Studies have also demonstrated that the first infection with rotavirus elicits a predominantly homotypic, serum-neutralizing antibody response to the virus, and subsequent infections elicit a broader, heterotypic response (19,23,58,63) Controversy exists as to whether serum antibodies are directly involved in protection or merely reflect recent infection. Review of data from a variety of studies of humans, including challenge experiments with adult volunteers, longitudinal studies of rotavirus infection in young children, and clinical trials of animal and animal-human reassortant rotavi- on May 10, 2018 by guest http://cmr.asm.org/ rus vaccines in infants, suggests that serum antibodies, if present at critical levels, are either protective themselves or an important and powerful correlate of protection against rotavirus disease, even though other host effectors may play an important role as well (40).…”

Section: Natural Protectionmentioning

confidence: 99%

Rotavirus Vaccines: an Overview

2008

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SUMMARY Rotavirus infection is the most common cause of severe diarrhea disease in infants and young children worldwide and continues to have a major global impact on childhood morbidity and mortality. Vaccination is the only control measure likely to have a significant impact on the incidence of severe dehydrating rotavirus disease. In 1999, a highly efficacious rotavirus vaccine licensed in the United States, RotaShield, was withdrawn from the market after 14 months because of its association with intussusception. Two new live, oral, attenuated rotavirus vaccines were licensed in 2006: the pentavalent bovine-human reassortant vaccine (RotaTeq) and the monovalent human rotavirus vaccine (Rotarix). Both vaccines have demonstrated very good safety and efficacy profiles in large clinical trials in western industrialized countries and in Latin America. Careful surveillance has not revealed any increased risk of intussusception in the vaccinated groups with either vaccine. The new rotavirus vaccines are now introduced for routine use in a number of industrialized and developing countries. These new safe and effective rotavirus vaccines offer the best hope of reducing the toll of acute rotavirus gastroenteritis in both developed and developing countries.

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“…Primary rotavirus infection usually results in production of NAb to the infecting serotype, although heterotypic NAb responses are also often detected (7,13,15,19,40,45,58). Subsequent rotavirus infection or inoculation with different serotypes has resulted in production of NAb to the new rotavirus strain and increased titers of antibody to other rotavirus serotypes, presumably due to anamnestic responses (4,8,15,34,51,55).…”

mentioning

confidence: 99%

Prevalence of Neutralizing Antibodies against Different Rotavirus Serotypes in Children with Severe Rotavirus-Induced Diarrhea and Their Mothers

Ray

Kelkar

2004

Clin Vaccine Immunol

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Neutralizing antibody (NAb) responses to different rotavirus serotypes were compared in 64 convalescentphase serum samples from hospitalized rotavirus-positive children less than 2 years of age and their mothers. Compared to the child patients, the mothers showed significantly higher NAb positivity to animal rotavirus serotypes G3 simian (96.88%), G6 bovine (85.94%), and G10 bovine (25.0%) and to human rotavirus serotypes G8 (79.69%) and G3 (57.81%) (P < 0.01 for each) but not to human serotypes G1, G2, G4, and G9 (P > 0.05). The overall prevalence of NAb among the child patients was low for human rotavirus serotypes G1 (20.31%) and G3 (21.8%). The comparative NAb response in individual mother-child paired serum samples was analyzed against each rotavirus serotype. A substantial number of child patients showed higher NAb titers than their mothers to serotypes G1, G2, G4, and G9, indicating that these serotypes are the major serotypes causing rotavirus diarrhea among the children of Pune, India. In these cases, the mothers were either negative or had lower titers of NAbs than their children. Correlation was observed between the infecting serotype and child patient serum that showed a homologous NAb response at a higher level than that of the mother. It appears that when the level of NAb to a particular serotype is higher among child patients than among their mothers, that serotype is the infecting serotype, and that low titers of NAb among the mothers predispose the children to infection with that serotype, if the serotype is in circulation.

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Diverse serologic response to rotavirus infection of infants in a single epidemic

Cited by 41 publications

References 0 publications

Serum Antibody Responses to Individual Viral Polypeptides in Human Rotavirus Infections

Serum Antibody Responses to Individual Viral Polypeptides in Human Rotavirus Infections

Rotavirus Vaccines: an Overview

Prevalence of Neutralizing Antibodies against Different Rotavirus Serotypes in Children with Severe Rotavirus-Induced Diarrhea and Their Mothers

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