Diversidad Genética en México De Variedades Nativas De Chile ‘Poblano’ Mediante Microsatélites

Toledo, Aremi R. Contreras; Sánchez, Higinio López; Santacruz-Várela, Amalio; Valadez-Moctezuma, Ernestina; Rincón, Víctor Heber Aguilar; Corona-Torres, Tarsicio; López, Pedro Antonio

doi:10.35196/rfm.2011.4.225

Cited by 12 publications

(4 citation statements)

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“…According to Wright´s classification, the average differentiation between lines (F ST = 0.48) was high, compared to the F ST obtained in natural populations of C. chinense. A study conducted by Contreras-Toledo et al, (2011) obtained F ST values between 0.10 and 0.17, as explained by the effect of gene flow between the five evaluated populations. The opposite occurred for the three lines in the present research, where the gene flow was very restricted.…”

Section: Discussionmentioning

confidence: 88%

SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae)

Lopéz-Candelo

Viáfara-Vega

Cárdenas

2022

Rev. Colomb. Cienc. Hortic

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This study was the first evaluation of the genetic diversity of cultivated populations of habanero pepper (Capsicum chinense) in Colombia using SSR-HRM. Three habanero pepper lines were characterized with eight microsatellite markers using the High-Resolution Melt (HRM) technique. Twenty-seven individuals from the HL-original line and 30 individuals each from derived lines HL-70 and HL67 were genotyped. Three microsatellites were monomorphic, and five were polymorphic; however, a high allelic diversity was detected in the homozygous state in the 87 individuals. The Ng8 marker differentiated the HL-original and HL-67 lines from the HL-70 line with HRM profiles. The analysis of molecular variance (AMOVA) revelated that 52% of the genetic variation existed within lines. The HL-67 line was more similar to the HL-original line than to the HL-70 line. HL-70 recorded the highest genetic diversity for the derived lines and, therefore, could be used in a new breeding program. In contrast, the HL-67 line, because of its high genetic homogeneity, could potentially be used to evaluate different environmental conditions to find optimal conditions that increase productivity and pungency. Finally, comparing the HRM profiles with the monomorphic markers (Ng 33, Ng 18 and Ng 10) differentiated the C. chinense and C. frutescens species, which was difficult because of high morphologic similarity between these two species and is usually evaluated at the flowering stage, while HRM profiles can be done at any plant stage.

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Section: Discussionmentioning

confidence: 88%

SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae)

Lopéz-Candelo

Viáfara-Vega

Cárdenas

2022

Rev. Colomb. Cienc. Hortic

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“…La variación observada en los primeros tres componentes concuerda con la baja estructura genética encontrada en las poblaciones estudiadas. Valores inferiores fueron reportados por Contreras et al (2011) en chile poblano y ancho (C. annum L.), así como Toledo-Aguilar et al (2016) con chile ancho (C. annum L.). Sus valores explicaron el 50.5 % de la variación total observada, con 10 componentes principales, para lo cual usaron marcadores microsatélites (SSR).…”

Section: Relación Genética Interpoblacionalunclassified

“…entre ellos,Contreras et al (2011) en 55 poblaciones de chile Poblano del estado de Puebla reportaron 105 alelos en total y 5.53 alelos por locus para 19 loci SSR y polimorfismo de 80 %, el cual es inferior al encontrado en el presente trabajo. Toledo-Aguilar et al (2016) utilizaron 24 loci de SSR, para analizar la diversidad genética de chile ancho y encontraron 220 alelos, con un promedio de 9.2 alelos por locus en 44 accesiones de ese tipo de chile.Con el mismo tipo de marcador SSR, también se han reportado estudios de diversidad genética entre diferentes especies de Capsicum;por ejemplo, Dhaliwal et al (2014), en 64 accesiones de tres especies de Capsicum de la India (C. annuum, C. Chinense y C. utescens) utilizaron 27 loci SSR y obtuvieron 75 alelos en total y 2.78 alelos por locus.…”

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DIVERSIDAD GENÉTICA DE CHILE HABANERO (Capsicum chinense Jacq.) MEDIANTE ISSR

et al. 2018

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El chile habanero (Capsicum chinense Jacq.) es distintivo de la Península de Yucatán, México; sin embargo, los estudios de diversidad genética a nivel molecular en este cultivo son escasos. El presente trabajo se planteó con el objetivo de caracterizar la diversidad genética del germoplasma de chile habanero, mediante marcadores ISSR. El material genético consistió en 60 poblaciones criollas de chile habanero recolectadas en los estados de Yucatán, Campeche, Quintana Roo y Tabasco, México, las cuales fueron genéticamente analizadas con tres loci ISSRs. El ADN fue extraído por el método de CTAB y los fragmentos se amplificaron por la reacción en cadena de la polimerasa (PCR). Los datos se analizaron con los programas POPGENE V 1.31 y NTSYS V 2.0 mediante un análisis de componentes principales y las relaciones entre las poblaciones se obtuvieron con un análisis jerárquico de conglomerados usando el método UPGMA. Se detectaron un total 32 bandas y 98 % de ellas fueron polimórficas. Los resultados indican que la diversidad genética en poblaciones de chile habanero es alta, en donde el 95.5 % de la variación observada se encuentra dentro de las poblaciones y sólo el 4.5 % entre ellas. Se detectó un alto flujo génico entre las poblaciones. Los resultados de los análisis multivariados sugieren que gran cantidad de germoplasma de chile habanero se ha dispersado del estado de Yucatán a los estados de Campeche, Quintana Roo y Tabasco. El patrón de distribución de la diversidad genética de chile habanero no se asoció con el lugar de origen de las poblaciones analizadas.

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“…Inclusion of both semiwild and wild chile pepper types, along with diverse landraces will help us best capture existing genetic diversity in this system. Domestication and genetic diversity of C. annuum populations in Mexico have been studied with different molecular markers including isoenzymes, RAPDs, SSRs, and more recently with SNPs (Hernández-Verdugo et al, 2001;Oyama et al, 2006;Contreras et al, 2011;González-Jara et al, 2011;Pacheco-Olvera et al, 2012;Toledo-Aguilar et al, 2016;Taitano et al, 2019). Many previous studies have explored the structure and genetic diversity of wild pepper populations while analyzing only a small number of domesticated populations (Hernández-Verdugo et al, 2001;Oyama et al, 2006;González-Jara et al, 2011;Pacheco-Olvera et al, 2012).…”

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Genetic diversity, gene flow, and differentiation among wild, semiwild, and landrace chile pepper (Capsicum annuum) populations in Oaxaca, Mexico

Martínez

Eguiarte

Mercer

et al. 2022

American J of Botany

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Premise Capsicum annuum (Solanaceae) was originally domesticated in Mexico, where wild (C. annuum var. glabriusculum) and cultivated (C. annuum var. annuum) chile pepper populations (>60 landraces) are common, and wild‐resembling individuals (hereafter semiwild) grow spontaneously in anthropogenic environments. Here we analyze the role of elevation and domestication gradients in shaping the genetic diversity in C. annuum from the state of Oaxaca, Mexico. Methods We collected samples of 341 individuals from 28 populations, corresponding to wild, semiwild (C. annuum var. glabriusculum) and cultivated C. annuum, and closely related species Capsicum frutescens and C. chinense. From the genetic variation of 10 simple sequence repeat (SSR) loci, we assessed the population genetic structure, inbreeding, and gene flow through variance distribution analyses, genetic clustering, and connectivity estimations. Results Genetic diversity (HE) did not differ across domestication levels. However, inbreeding coefficients were higher in semiwild and cultivated chiles than in wild populations. We found evidence for gene flow between wild populations and cultivated landraces along the coast. Genetic structure analysis revealed strong differentiation between most highland and lowland landraces. Conclusions Gene flow between wild and domesticated populations may be mediated by backyards and smallholder farms, while mating systems may facilitate gene flow between landraces and semiwild populations. Domestication and elevation may overlap in their influence on genetic differentiation. Lowland Gui'ña dani clustered with highland landraces perhaps due to the social history of the Zapotec peoples. In situ conservation may play an important role in preserving semiwild populations and private alleles found in landraces.

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Diversidad Genética en México De Variedades Nativas De Chile ‘Poblano’ Mediante Microsatélites

Cited by 12 publications

References 2 publications

SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae)

SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae)

DIVERSIDAD GENÉTICA DE CHILE HABANERO (Capsicum chinense Jacq.) MEDIANTE ISSR

Genetic diversity, gene flow, and differentiation among wild, semiwild, and landrace chile pepper (Capsicum annuum) populations in Oaxaca, Mexico

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