Diversidade do potencial amilolítico em fungos filamentosos: purificação e caracterização de uma glucoamilase de Aspergillus brasiliensis

Almeida, Paula Zaghetto de

doi:10.11606/d.59.2015.tde-15052015-085904

Cited by 3 publications

(6 citation statements)

References 49 publications

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“…fungus. A similar result was obtained by Almeida (2015), who, in his research, performed the day test on two filamentous fungi Aspergillus brasiliensis and Rhizopus oryzae, and the ideal time for each was five and four days, respectively. Paris et al (2012) found that the best growth time for the production of amylases, proteases and lipases by Aspergillus niger was on the sixth day.…”

Section: Mediumsupporting

confidence: 79%

“…Almeida 2015, Silva (2009) and Facchini (2010) demonstrated the superiority of Aspergillus filamentous fungi, compared to other genera, in the production of enzymes. Almeida (2015) reports that the amylolytic activity achieved by Aspergillus brasiliensis under identical conditions corresponded to 24.36% of the activity shown by the filamentous fungus Aspergillus sp. M1.11 and about 2.1% of the activity of Aspergillus sp.…”

Section: Selection Of Filamentous Amylase-producing Fungimentioning

confidence: 95%

“…The results obtained regarding the collected genus were similar to those found by several authors. The predominantly isolated fungi found in sampling made in various parts of the country were cosmopolitan Aspergillus and Penicillium (Cavalcanti et al, 2006;Gomes, 2007;Benassi et al, 2014;Almeida, 2015;Griebeler, et al, 2015, Pasin et al, 2020. Pelczar et al (1997) divided the organisms into three classes, according to the ideal growth temperature: psychrophiles (temperatures below 20 °C), mesophiles (between 25 and 40 °C) and thermophiles (temperatures above 40 °C).…”

Section: Sampling Isolation and Identification Of Fungimentioning

confidence: 99%

“…This fact represents a greater savings in the cost of producing enzymes. Almeida (2015) tested the Khanna, SR and CP media without any modifications. For Aspergillus brasiliensis, the greatest production of amylases was observed under static conditions in SR, CP and Khanna media, respectively, where the variation in activity between the two best media was very small, considering the difference between the compositions.…”

Section: Definition Of the Culture Medium And The Salt Solution For Cmentioning

confidence: 99%

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PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

Souza¹,

Marinho²,

Pasin³

et al. 2020

jCEC

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Amylases are used in numerous industrial applications for converting starch into products of greater value. This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize the parameters for the cultivation of the microorganism and biochemically characterize the amylase. Among 21 filamentous fungi isolated in Janaúba, state of Minas Gerais, Brazil, the best amylase producer was selected for standardization of culture parameters and subsequent enzymatic characterization. Maximum activity was obtained in CP medium after six days of cultivation at 30 °C. Amylases produced by this fungus are stable to variations in pH and temperature, exhibited optimum activities at 65 oC and pH 6.0, and were significantly activated in the presence of 5 and 10 mm KH2PO4.

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Section: Mediumsupporting

confidence: 79%

Section: Selection Of Filamentous Amylase-producing Fungimentioning

confidence: 95%

Section: Sampling Isolation and Identification Of Fungimentioning

confidence: 99%

Section: Definition Of the Culture Medium And The Salt Solution For Cmentioning

confidence: 99%

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PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

Souza¹,

Marinho²,

Pasin³

et al. 2020

jCEC

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“…Podem também associar-se simbioticamente com raízes de plantas, formando micorrizas (Grimm et al, 2005;Strullu-Derrien & Strullu, 2007). (Varga et al, 2007;Almeida, 2015).…”

Section: 8o Fungo Filamentoso Aspergillus Brasiliensisunclassified

Imobilização e caracterização bioquímica de mananases e pectinases produzidas por Aspergillus brasiliensis: potencial para aplicação na clarificação de sucos de frutas

Infante¹

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e dedicação que tiveram ao decorrer da minha formação pessoal e educacional, sempre me apoiando para seguir o meu sonho mesmo que a 2 mil Km de distância. À minha irmã Roberta, cunhado Alexandre, meu irmão Marcus Vinicius e cunhada Thais por todo carinho, amizade e momentos de conversas e diversão. Aos meus sobrinhos Letizia Marie e Rafael Henrique por simplesmente existirem na minha vida. Amo todos vocês! Á Profa. Dra. Maria de Lourdes T. de M. Polizeli pela orientação, paciência e apoio durante esses 4 anos de convivência. Por acreditar no meu potencial e crescimento, contribuindo muito para minha formação.

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Fungal amylases applied to the sweet potato starch for bioethanol production

Romão

Filho

Tininis

et al. 2022

RSD

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Bioethanol is a sustainable energy source to help reducing the emission of pollutants into the global environment. In order to cope with that, the ethanol production technologies and use of efficient and low-cost substrates are developed. The objective of this research was to evaluate fungal amylases in sweet potato starch for bioethanol production by Saccharomyces cerevisiae in the laboratory. Endomelanconiopsis endophytica (1.40 U/mL), Neopestalotiopsis cubana (1.67 U/mL) and Fusarium pseudocircinatum (1.11 U/mL) with high enzymatic activities were selected and their amylases were tested for activity on sweet potato starch for bioethanol production. Simultaneous saccharification and fermentation was performed at 30° C and pH = 5.0. 17.3 - 88.1 (%) of bioethanol that was produced and compared to the expected theoretical yield. Therefore, amylases from these fungi simultaneously inserted on sweet potato starch and S. cerevisiae are potentially useful for bioethanol production.

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Diversidade do potencial amilolítico em fungos filamentosos: purificação e caracterização de uma glucoamilase de Aspergillus brasiliensis

Cited by 3 publications

References 49 publications

PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

Imobilização e caracterização bioquímica de mananases e pectinases produzidas por Aspergillus brasiliensis: potencial para aplicação na clarificação de sucos de frutas

Fungal amylases applied to the sweet potato starch for bioethanol production

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