Diversification of Root Hair Development Genes in Vascular Plants

Huang, Ling; Shi, Xinhui; Wang, Wenjia; Ryu, Kook Hui; Schiefelbein, John

doi:10.1104/pp.17.00374

Cited by 37 publications

(44 citation statements)

References 63 publications

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“…However, nothing is known about the negative regulation of RSL class I genes in any other land plant. The role of GL2 as negative regulator of RSL class I genes and root hair development is not likely to be widely conserved because the closest homologs of GL2 in many other vascular plants are not expressed in roots ( Huang et al, 2017 ). Furthermore, GL2 genes have not been identified in bryophytes (liverworts, mosses and hornworts) ( Zalewski et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants

Honkanen

Thamm

Arteaga-Vázquez

et al. 2018

eLife

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Basic helix-loop-helix transcription factors encoded by RSL class I genes control a gene regulatory network that positively regulates the development of filamentous rooting cells – root hairs and rhizoids – in land plants. The GLABRA2 transcription factor negatively regulates these genes in the angiosperm Arabidopsis thaliana. To find negative regulators of RSL class I genes in early diverging land plants we conducted a mutant screen in the liverwort Marchantia polymorpha. This identified FEW RHIZOIDS1 (MpFRH1) microRNA (miRNA) that negatively regulates the RSL class I gene MpRSL1. The miRNA and its mRNA target constitute a feedback mechanism that controls epidermal cell differentiation. MpFRH1 miRNA target sites are conserved among liverwort RSL class I mRNAs but are not present in RSL class I mRNAs of other land plants. These findings indicate that while RSL class I genes are ancient and conserved, independent negative regulatory mechanisms evolved in different lineages during land plant evolution.

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Section: Introductionmentioning

confidence: 99%

Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants

Honkanen

Thamm

Arteaga-Vázquez

et al. 2018

eLife

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“…FEBS Letters, 553(1-2): 113-118 ALA family and other P-type ATPases generated in Arabidopsis Heat Tree Viewer (http://arabidopsis-heattree.org), with ALA clusters (Baxter et al, 2003) bracketed by grey rectangles and thicker lines representing bootstrap values of 98 or greater. (B) Database expression profile of ALA4/5/6/7 genes in rosette, root, hypocotyl, trichomes, and mature pollen, generated from Arabidopsis eFP browser (Winter et al, 2007), with root hair data from Huang et al, 2017 Figure 5. Ectopic expression of ALA4 in pollen restores seed set and normal pollen transmission in an ala6/7 double KO.…”

Section: Acknowledgementsmentioning

confidence: 99%

The Lipid Flippases ALA4 and ALA5 Play Critical Roles in Cell Expansion and Plant Growth

et al. 2020

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A subclass of lipid flippases contribute to glycerolipid and sphingolipid homeostasis in Arabidopsis leaves, and play critical roles in cell expansion and vegetative growth. Footnotes: Author contributions: J.D. authored the manuscript. R.P. and J.D. analyzed vegetative physiology. S.M. bred the double-knockout lines and identified dwarf mutants. E.B., A.R., and J.S. performed subcellular localization experiments. L.P. and R.L. were involved in the cloning of ALA5 for yeast expression. T.B. performed lipid uptake and functional complementation assays. R.L. analyzed the data and carried out statistical analysis for yeast experiments. M.P. and R.L. supervised yeast experiments. E.C. and R.C. performed sphingolipid profiling of ala4/5 mutants. J.H. performed all plant crosses, supervised in planta experiments, and assisted J.D. in writing. J.H. agrees to serve as author responsible for contact.

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“…However, plant-specific Class III peroxidase can also generate ROS (Shigeto and Tsutsumi, 2016). Accordingly, several Class III peroxidase genes are highly induced during root hair development in higher plants (Huang et al, 2017;Moon et al, 2018). Results from our microarray and RT-qPCR analyses of the root hairs from Os07g39940-OX indicated that three genes encoding Class III peroxidase were up-regulated in the transgenics (Fig.…”

Section: Rsl Class II Subfamily Genes and Rhl1 Regulate Ros Levels Inmentioning

confidence: 82%

RSL Class II Transcription Factors Guide the Nuclear Localization of RHL1 to Regulate Root Hair Development

et al. 2018

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Root hairs are important for absorption of nutrients and water from the rhizosphere. The Root Hair Defective-Six Like (RSL) Class II family of transcription factors is expressed preferentially in root hairs and has a conserved role in root hair development in land plants. We functionally characterized the seven members of the RSL Class II subfamily in the rice (Oryza sativa) genome. In root hairs, six of these genes were preferentially expressed and four were strongly expressed. Phenotypic analysis of each mutant revealed that Os07g39940 plays a major role in root hair formation, based on observations of a short root hair phenotype in those mutants. Overexpression (OX) for each of four family members in rice resulted in an increase in the density and length of root hairs. These four members contain a transcription activation domain and are targeted to the nucleus. They interact with rice Root Hairless1 (OsRHL1), a key regulator of root hair development. When heterologously expressed in epidermal cells of Nicotiana benthamiana leaves, OsRHL1 was predominantly localized to the cytoplasm. When coexpressed with each of the four RSL Class II members, however, OsRLH1 was translocated to the nucleus. Transcriptome analysis using Os07g39940-OX plants revealed that 86 genes, including Class III peroxidases, were highly up-regulated. Furthermore, reactive oxygen species levels in the root hairs were increased in Os07g39940-OX plants but were drastically reduced in the os07g39940 and rhl1 mutants. Our results demonstrate that RSL Class II members function as essential regulators of root hair development in rice.

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Diversification of Root Hair Development Genes in Vascular Plants

Cited by 37 publications

References 63 publications

Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants

Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants

The Lipid Flippases ALA4 and ALA5 Play Critical Roles in Cell Expansion and Plant Growth

RSL Class II Transcription Factors Guide the Nuclear Localization of RHL1 to Regulate Root Hair Development

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