Diversified component incorporated hybrid nanoflowers: A versatile material for biosensing and biomedical applications

Dang, Thinh Viet; Kim, Moon Il

doi:10.1007/s11814-022-1292-z

Cited by 9 publications

(2 citation statements)

References 69 publications

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“…The linear ranges were 0 to 40 and 0 to 4 nM, respectively. Recently, the FMD gene detection was investigated as an LOD of 0.3 fM and 0 to 1 nM of linear range using amperometry based on gold nanostructures [ 36 , 37 ]. However, since the detection of the genes required amplification steps that were time-consuming and equipment-demanding, the peptide detection was considered more convenient in terms of field-friendly methodology.…”

Section: Resultsmentioning

confidence: 99%

Surface-Plasmon-Resonance Amplification of FMD Detection through Dendrimer Conjugation

Jung,

Park

2024

Sensors

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The amplification of the surface plasmon resonance (SPR) sensitivity for the foot-and-mouth disease (FMD) detection was studied using Poly(amidoamine) (PAMAM) succinamic-acid dendrimers. The dendrimers were conjugated with the complementary annealed with the aptamers capable of binding specifically to FMD peptides. The tethered layer of the dendrimer-conjugated double-stranded(ds)-aptamers was formed on the SPR sensor Au surface via a thiol bond between the aptamers and Au. After the tethered layer was formed, the surface was taken out of the SPR equipment. Then, the ds-aptamers on the surface were denatured to collect the dendrimer-conjugated single-stranded(ss)-complementary. The surface with only the remaining ss-aptamers was transferred again to the equipment. Two types of the injections, the FMD peptide only and the dendrimer-conjugated ss-complementary followed by the FMD peptides, were performed on the surface. The sensitivity was increased 20 times with the conjugation of the dendrimers, but the binding rate of the peptides became more than two times slower.

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Section: Resultsmentioning

confidence: 99%

Surface-Plasmon-Resonance Amplification of FMD Detection through Dendrimer Conjugation

Jung,

Park

2024

Sensors

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“…Notably, the differences in DNA sequence and length could affect the morphology of the nanospheres. The preparation could be conducted facilely under mild conditions based on the coordination interaction between metal ions (such as Cu, Zn, Fe, Co, Ca, and Mn) and amide and amine groups in DNA organic ligands [41], in which DNA-Mn hybrid nanospheres showed potential as a cell tracking agent and a contrast agent for magnetic resonance imaging (MRI) [22]. Compared with using DNA as a linker to assemble nanospheres, hybrid nanospheres provide many advantageous properties such as simplicity, resistance against nucleases, and large loading capacities [40].…”

Section: Self-assembly Of Metal-dna Coordinationmentioning

confidence: 99%

Self-Assembled DNA Nanospheres: Design and Applications

Li,

Liu,

Wang

et al. 2023

Chemistry

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Self-assembled DNA nanospheres, as versatile and ideal vehicles, have offered new opportunities to create intelligent delivery systems for precise bioimaging and cancer therapy, due to their good biostability and cell permeability, large loading capacity, and programmable self–assembly behaviors. DNA nanospheres can be synthesized by the self–assembly of Y–shaped DNA monomers, ultra–long single-stranded DNA (ssDNA), and even metal–DNA coordination. Interestingly, they are size–controllable by varying some parameters including concentration, reaction time, and mixing ratio. This review summarizes the design of DNA nanospheres and their extensive biomedical applications. First, the characteristics of DNA are briefly introduced, and different DNA nanostructures are mentioned. Then, the design of DNA nanospheres is emphasized and classified into three main categories, including Y–shaped DNA unit self-assembly by Watson–Crick base pairing, liquid crystallization and the dense packaging of ultra–long DNA strands generated via rolling circle amplification (RCA), and metal–DNA coordination–driven hybrids. Meanwhile, the advantages and disadvantages of different self–assembled DNA nanospheres are discussed, respectively. Next, the biomedical applications of DNA nanospheres are mainly focused on. Especially, DNA nanospheres serve as promising nanocarriers to deliver functional nucleic acids and drugs for biosensing, bioimaging, and therapeutics. Finally, the current challenges and perspectives for self-assembled DNA nanospheres in the future are provided.

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Ficin-copper hybrid nanoflowers with enhanced peroxidase-like activity for colorimetric detection of biothiols

Dang,

Kim,

Kim

2023

Microchim Acta

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Diversified component incorporated hybrid nanoflowers: A versatile material for biosensing and biomedical applications

Cited by 9 publications

References 69 publications

Surface-Plasmon-Resonance Amplification of FMD Detection through Dendrimer Conjugation

Surface-Plasmon-Resonance Amplification of FMD Detection through Dendrimer Conjugation

Self-Assembled DNA Nanospheres: Design and Applications

Ficin-copper hybrid nanoflowers with enhanced peroxidase-like activity for colorimetric detection of biothiols

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