Diversified metabolism makes novel Thauera strain highly competitive in low carbon wastewater treatment

Ren, Tong; Chi, Yulei; Wang, Yu; Shi, Xuan; Jin, Xin; Jin, Pengkang

doi:10.1016/j.watres.2021.117742

Cited by 161 publications

(26 citation statements)

References 92 publications

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“…For example, Thauera has been identified in several types of wastewater treatments, including in microalgae–bacteria photobioreactors treating swine wastewater [ 10 ]. It is capable of heterotrophic denitrification using a variety of organic compounds, including extracellular polymeric substances, as carbon sources while respiring nitrate or nitrite [ 49 ], it has also been associated with the heterotrophic nitrification–aerobic denitrification process [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Current Concentrations of Zn, Cu, and As in Piggery Wastewater Compromise Nutrient Removals in Microalgae–Bacteria Photobioreactors Due to Altered Microbial Communities

Collao

García‐Encina

Blanco

et al. 2022

Biology

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The treatment of pig manure is a major environmental issue, and photobioreactors containing consortia of microalgae and bacteria have proven to be a promising and sustainable treatment alternative. This work studies the effect of Cu, Zn and As, three toxic elements frequently present in piggery wastewater, on the performance and microbiome of photobioreactors. After dopage with Zn (100 mg/L), Cu (100 mg/L), and As (500 µg/L), the high biomass uptake of Zn (69–81%) and Cu (81–83%) decreased the carbon removal in the photobioreactors, inhibited the growth of Chlorella sp., and affected heterotrophic bacterial populations. The biomass As uptake result was low (19%) and actually promoted microalgae growth. The presence of Cu and As decreased nitrogen removal, reducing the abundance of denitrifying bacterial populations. The results showed that metal(loid)s significantly affected 24 bacterial genera and that they did not recover after exposure. Therefore, this study makes an important contribution on the impact of the presence of metal(loid)s in piggery wastewater that compromises the overall performance of PBRs, and so, the environmental and health impact of treated effluents.

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Section: Discussionmentioning

confidence: 99%

Current Concentrations of Zn, Cu, and As in Piggery Wastewater Compromise Nutrient Removals in Microalgae–Bacteria Photobioreactors Due to Altered Microbial Communities

Collao

García‐Encina

Blanco

et al. 2022

Biology

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“…One probable reason could be the aerobic growth condition that we set for antibiotic treatment for retrieving drug-resistance bacteria [39,42]. Similarly, the second-most abundant group in our collection, namely the genus Thauera, was also not determined in our culturomics data for the same reason that Thauera is normally reported in anaerobic sludge [43,44].…”

Section: Identi Cation Of Pathogenic Genotypes In Culturomics Metagen...mentioning

confidence: 96%

Shotgun Metagenomics Unravels the Bacterial Diversity, Resistome Profile and Genetic Exchange in a Tropical Urban Environment

Salam

Philip

Prasad

et al. 2023

Preprint

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Background The environmental microbiome has a direct influence on human health and disease. Previous reports suggest that urbanization and anthropogenic activities can alter natural microbial flora and potentially spread infectious disease-causing agents by emergence of pathogenic strains of bacteria. The nature of microbes present in urban settings and the flow of genetic elements between environmental and clinically relevant pathogenic bacteria, however, remains largely unknown. Results To unravel the bacterial diversity and resistome profile of multiple hotspot setups of a tropical urban system such as transport hubs, wet markets, hospital surroundings, waste dumps, and urban coastal area (beaches) metagenomics analyses of sediment samples from around Thiruvananthapuram city were done. Our study revealed the presence of 3,735 species belonging to 46 phyla of bacteria and archaea. The phylum Pseudomonadota was the most abundant bacterial community, followed by Bacteriodota and Actinomycetota. The genus Cloacibacterium had the highest overall relative abundance, while Pseudomonas was the most prevalent bacterial genus in hospital surroundings and coastal area (beaches) settings. We identified 291 antimicrobial resistance genes (ARGs) in the urban resistome, conferring resistance to more than 15 drug classes. The hospital settings had the highest number of ARGs across different drug classes. From the culturomics microcosm setups, we reconstructed 62 high-quality metagenome-assembled genomes (MAGs) which shows high resemblance to pathogenic bacterias such as Klebsiella pneumoniae, Escherichia coli and Acinetobacter baumannii etc. The ARGs detected in these genomes include genes encoding antibiotic-modifying enzymes (ArnT, eptA, eptB), beta-lactamase (ampC, ampC1, ampH), transcription regulating proteins (KpnE, KpnF, KpnG), efflux pumps (oqxA, oqxB). Furthermore, eight MAGS belonging to Acinetobacter kookii, Acinetobacter pitti, Acinetobacter baumannii, Acinetobacter gerneri, Escherichia coli, Klebsiella pneumoniae and Klebsiella quasipneumoniae were found to contain virulence factors such as siderophores (acinetobactin, aerobactin, enterobactin etc.), capsule, secretion systems belonging to type III group) (T3SS, TTSS etc) or type II (T2SS), fimbriae (type 3 and I), efflux pump (AdeFGH), or quorum sensing (abaR) associated with pathogenicity. Conclusions The study provides insights into bacterial composition, antimicrobial resistance, and virulence potential in urban environments, highlighting the importance of monitoring and managing antimicrobial resistance in urban ecosystems.

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“…37 Carbohydrates (especially glucose) are considered to be easily utilized by denitrifiers. 38,39 3.3. Responses of Microcystis to Nitrogen Deficiency and Its Metabolites.…”

Section: Effects Of Microcystis On Nitrogen Reduction In the Whole Wa...mentioning

confidence: 99%

“…Reported studies support the idea that algae-produced DOC-contained amounts of carbohydrates, 10,47 and carbohydrates are considered to be easily utilized by denitrifying bacteria. 38,39 3.4. Mechanism for Nitrogen Removal Driven by Carbon/Nitrogen Coupling Metabolism of Microcystis.…”

Section: Effects Of Microcystis On Nitrogen Reduction In the Whole Wa...mentioning

confidence: 99%

Cyanobacterial Blooms Enhance Nitrogen Removal in Lakes through Carbon/Nitrogen Coupling Metabolism

Huang,

Chen,

Ostrovsky

2023

ACS EST Water

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Cyanobacterial blooms can promote nitrogen removal; however, the underlying mechanism remains a mystery. The present study suggested that the carbon−nitrogen coupling metabolism should play a critical role in nitrogen removal in eutrophic lakes. Field investigations illustrated that the carbohydrate content of natural Microcystis negatively correlated with the DIN concentration in spatiotemporal distribution. Under N-deficient conditions, the carbohydrate content of natural Microcystis increased from 10.5% to 44.1% after 4 days. The metabolomic analysis showed ten differential carbohydrate metabolites, of which seven were upregulated. More carbohydrate-rich Microcystis biomass might sink onto the bottom under a low-nitrogen condition; thus, nitrogen was removed more efficiently due to the enhancement of denitrification. This was further supported by enclosure experiments with Microcystis in which TN was removed more efficiently under low-nitrogen conditions (40.6%) than under high-nitrogen concentrations (17.4%). These results illustrated that the carbon/ nitrogen coupling mechanisms in intracellular physiological processes of Microcystis and in lake ecosystems were tightly linked, especially since lakes suffer from eutrophication. The present study provided field evidence for carbohydrate accumulation in Microcystis at nitrogen deficiency and indicated that the cyanobacteria−carbon interactions maintain the lake's high potential to remove nitrogen via denitrification.

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Diversified metabolism makes novel Thauera strain highly competitive in low carbon wastewater treatment

Cited by 161 publications

References 92 publications

Current Concentrations of Zn, Cu, and As in Piggery Wastewater Compromise Nutrient Removals in Microalgae–Bacteria Photobioreactors Due to Altered Microbial Communities

Current Concentrations of Zn, Cu, and As in Piggery Wastewater Compromise Nutrient Removals in Microalgae–Bacteria Photobioreactors Due to Altered Microbial Communities

Shotgun Metagenomics Unravels the Bacterial Diversity, Resistome Profile and Genetic Exchange in a Tropical Urban Environment

Cyanobacterial Blooms Enhance Nitrogen Removal in Lakes through Carbon/Nitrogen Coupling Metabolism

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