Diversified pattern of the human colorectal cancer microbiome

Geng, Jiawei; Fan, Hong; Tang, Xiaodan; Zhai, Huiqin; Zhang, Zhigang

doi:10.1186/1757-4749-5-2

Cited by 114 publications

(94 citation statements)

References 21 publications

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“…Fusobacterium has been associated with colorectal tumors and adenomas in several recent studies. 27,31,[61][62][63][64][65] In the present study, Fusobacterium was significantly over represented in tumor compared to adjacent tissues in the Spanish cohort, while in the US, this phylum was more abundant in both tissues compared to the Spanish cohort, but enriched specifically in tumors in splenic flexure, sigmoid and rectum. More studies are needed to assess the biological significance of these results, which could be related to the ability of these microorganisms to form and maintain biofilms in specific locations of the large intestine as recently shown.…”

Section: Discussionmentioning

confidence: 64%

Gut microbiome compositional and functional differences between tumor and non-tumor adjacent tissues from cohorts from the US and Spain

Delgado²,

et al. 2015

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Colorectal cancer (CRC) is the third most common cancer in the world and the second leading cause of cancer deaths in the US and Spain. The molecular mechanisms involved in the etiology of CRC are not yet elucidated due in part to the complexity of the human gut microbiota. In this study, we compared the microbiome composition of 90 tumor and matching adjacent tissue (adjacent) from cohorts from the US and Spain by 16S rRNA amplicon sequencing in order to determine the impact of the geographic origin on the CRC microbiome. Data showed a significantly (P < 0.05) higher Phylogenetic Diversity ( Predicted metagenome functional content from 16S rRNA surveys showed that bacterial motility proteins and proteins involved in flagellar assembly were over represented in adjacent tissues of both cohorts, while pathways involved in fatty acid biosynthesis, the MAPK signaling pathway, and bacterial toxins were over represented in tumors. Our study suggests that microbiome compositional and functional dissimilarities by geographic location should be taken in consideration when approaching CRC therapeutic options.

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Section: Discussionmentioning

confidence: 64%

Gut microbiome compositional and functional differences between tumor and non-tumor adjacent tissues from cohorts from the US and Spain

Delgado²,

et al. 2015

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“…This genus has been recurrently associated with CRC [20, 48, 50–53, 57, 96–98]. Moreover, Fusobacterium was not detected in healthy control samples, as it is a relatively uncommon bacterium in the gut microbiome.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiome of Moroccan colorectal cancer patients

Allali

Benkeblia

Bouguenouch

et al. 2018

Med Microbiol Immunol

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Although colorectal cancer is the third leading cause of death in Morocco, there are no studies of the microbiome changes associated with the disease in the Moroccan population. The aim of our study was to compare the stool microbiome of Moroccan cancer patients with healthy individuals. We analyzed the microbiome composition of samples from 11 CRC patients and 12 healthy individuals by 16S rRNA amplicon sequencing. Principal coordinate analysis of samples revealed defined cancer versus healthy clusters. Our findings showed that cancer samples had higher proportions of Firmicutes (T = 50.5%; N = 28.4%; p = 0.04), specifically of Clostridia (T = 48.3%; N = 19.0%; p = 0.002), and Fusobacteria (T = 0.1%; N = 0.0%; p = 0.02), especially of Fusobacteriia (T = 0.1%; N = 0.0%; p = 0.02), while Bacteroidetes were enriched in healthy samples (T = 35.1%; N = 62.8%; p = 0.06), particularly the class Bacteroidia (T = 35.1%; N = 62.6%; p = 0.06). Porphyromonas, Clostridium, Ruminococcus, Selenomonas, and Fusobacterium were significantly overrepresented in diseased patients, similarly to other studies. Predicted functional information showed that bacterial motility proteins, flagellar assembly, and fatty acid biosynthesis metabolism were significantly overrepresented in cancer patients, while amino acid metabolism and glycan biosynthesis were overrepresented in controls. This suggests that involvement of these functional metagenomes is similar and relevant in the carcinogenesis process, independent of the origin of the samples. Results from this study allowed identification of bacterial taxa relevant to the Moroccan population and encourages larger studies to facilitate population-directed therapeutic approaches.Electronic supplementary materialThe online version of this article (10.1007/s00430-018-0542-5) contains supplementary material, which is available to authorized users.

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“…The best know example is the role Helicobater pylori plays in gastric cancer [43][44][45]. However, a handful of laboratories have reported links between bacterial infection and oral [11], colon [46][47][48][49], pancreatic [46,50], liver [51], esophageal [52] and prostate cancers [53]. The biological mechanism of these associations is not yet understood [54,55], however, there are several mechanisms by which bacterial infection could lead to the initiation and progression of oncogenic processes [42,56,57].…”

Section: Discussionmentioning

confidence: 99%

16S rRNA amplicon sequencing identifies microbiota associated with oral cancer, Human Papilloma Virus infection and surgical treatment

et al. 2016

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Systemic inflammatory events and localized disease, mediated by the microbiome, may be measured in saliva as head and neck squamous cell carcinoma (HNSCC) diagnostic and prognostic biomonitors. We used a 16S rRNA V3-V5 marker gene approach to compare the saliva microbiome in DNA isolated from Oropharyngeal (OPSCC), Oral Cavity Squamous Cell Carcinoma (OCSCC) patients and normal epithelium controls, to characterize the HNSCC saliva microbiota and examine their abundance before and after surgical resection.The analyses identified a predominance of Firmicutes, Proteobacteria and Bacteroidetes, with less frequent presence of Actinobacteria and Fusobacteria before surgery. At lower taxonomic levels, the most abundant genera were Streptococcus, Prevotella, Haemophilus, Lactobacillus and Veillonella, with lower numbers of Citrobacter and Neisseraceae genus Kingella. HNSCC patients had a significant loss in richness and diversity of microbiota species (p<0.05) compared to the controls. Overall, the Operational Taxonomic Units network shows that the relative abundance of OTU's within genus Streptococcus, Dialister, and Veillonella can be used to discriminate tumor from control samples (p<0.05). Tumor samples lost Neisseria, Aggregatibacter (Proteobacteria), Haemophillus (Firmicutes) and Leptotrichia (Fusobacteria). Paired taxa within family Enterobacteriaceae, together with genus Oribacterium, distinguish OCSCC samples from OPSCC and normal samples (p<0.05). Similarly, only HPV positive samples have an abundance of genus Gemellaceae and Leuconostoc (p<0.05). Longitudinal analyses of samples taken before and after Research PaperOncotarget 51321 www.impactjournals.com/oncotarget surgery, revealed a reduction in the alpha diversity measure after surgery, together with an increase of this measure in patients that recurred (p<0.05). These results suggest that microbiota may be used as HNSCC diagnostic and prognostic biomonitors.

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Diversified pattern of the human colorectal cancer microbiome

Cited by 114 publications

References 21 publications

Gut microbiome compositional and functional differences between tumor and non-tumor adjacent tissues from cohorts from the US and Spain

Gut microbiome compositional and functional differences between tumor and non-tumor adjacent tissues from cohorts from the US and Spain

Gut microbiome of Moroccan colorectal cancer patients

16S rRNA amplicon sequencing identifies microbiota associated with oral cancer, Human Papilloma Virus infection and surgical treatment

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