Diversity among wild accessions of Bacopa monnieri (L.) Wettst. and their morphogenetic potential

Bansal, Mahima; Kumar, Anil; Reddy, M. Sudhakara

doi:10.1007/s11738-014-1493-0

Cited by 19 publications

(11 citation statements)

References 37 publications

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“…accession BM6 was selected for the present study (Bansal et al 2014). All routine chemicals were purchased from HiMedia Laboratories (Mumbai, India); growth regulators and antibiotics were purchased from Sigma Chemical Co. (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Bacopa monnieri (L.) Wettst, family Scrophulariaceae (common name brahmi) is the only source of bacosides, which possesses legendary reputation as a memory vitalizer with a proven nootropic action (Anonymous 1988). The major chemical entity responsible for its memoryfacilitating action is 'bacoside A', which is usually known to co-occur with 'bacoside B' (Chatterji et al 1965), latter differing only in optical rotation (Rastogi 1990).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’ production from Bacopa monnieri (L.) Wettst.

2014

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Stable lines of hairy roots were established from leaf explants of Bacopa monnieri using different strains (A4, R1000, SA79, MTCC 532 and MTCC 2364) of Agrobacterium rhizogenes. The efficiency of hairy roots induction of these strains varied significantly and the maximum transformation frequency (75 %) was observed in case of strain SA79 using leaf explants followed by internode (55 %) in the presence of acetosyringone. Different parameters such as cell density of Agrobacterium suspension, co-cultivation period and infection time influenced the root induction frequency. Maximum frequency of root induction was obtained with bacterial density of 0.6 OD 600 , 2 days of co-cultivation period and 10 min of infection time. Integration of T-DNA in the genome of hairy roots was confirmed by PCR amplification of rolB gene. Elimination of Agrobacterium from the established root cultures was ascertained by amplifying the DNA fragment specific to 16S rDNA and virD gene. All lines of hairy roots except strain A4 induced showed higher growth rate and accumulated higher levels of 'bacoside A' than the untransformed roots. Maximum biomass accumulation (6.8 g l -1 ) and 'bacoside A' content (10.02 mg g -1 DW) were recorded in case of the hairy root line induced by strain MTCC 2364.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’ production from Bacopa monnieri (L.) Wettst.

2014

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“…Thus the observed variations could only be due to genotypic differences of these accessions. Genetic variation in these accessions were reported earlier (Bansal et al 2014).…”

Section: Resultsmentioning

confidence: 57%

“…Bacoside A was extracted from the samples and purified following the procedure described earlier (Bansal et al 2014). Briefly, samples (1.0 g dried powder in triplicate) were soaked in 10.0 ml water for 24 h, filtered through glass wool and filtrates were discarded.…”

Section: Extraction Of Bacoside Amentioning

confidence: 99%

“…It is estimated that the yearly market demand for its raw material is about 1000 tons, which is increasing at a steady pace (National Medicinal Plants Board and Department of Science and Technology, Government of India 2007). This heavy demand of raw material is entirely met from wild populations, and levels of active principle in the raw material collected from the wild populations have been reported to show considerable variations (Nadeem et al 2007), which could be due to genotypic variations (Bansal et al 2014) and also the season of harvest (Walker et al 2001). Because all the above ground parts of B. monnieri have been reported to contain active principle and therefore, total harvestable biomass along with levels of bacoside A are important for the yield of active principle.…”

Section: Introductionmentioning

confidence: 99%

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Seasonal variations in harvest index and bacoside A contents amongst accessions of Bacopa monnieri (L.) Wettst. collected from wild populations

Bansal

Reddy

Kumar

2016

Physiol Mol Biol Plants

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Bacoside A, a major active principle of Bacopa monnieri known for its cognitive effects is a mixture of saponins like bacoside A3, bacopaside II, isomer of bacopasaponin C and bacopasaponin C. Seasonal changes in biomass and bacoside A levels in fourteen accessions of B. monnieri were evaluated after maintaining these at a common site at Thapar University campus, Patiala (30°19 0 36.12 00 N and 76°24 0 1.08 00 E) for 1 year. Harvestable biomass and total bacoside A contents varied significantly between the accessions and also in a particular accession during different seasons of the year. The maximum dry weight of plant (biomass 1.64 g) and bacoside A levels (6.82 mg/plant) were recorded in accession BM1. Harvestable biomass was highest during summer in accessions BM1 and BM7 (FW 4.2 g/plant), whereas bacoside A levels were also highest during summer and in accession BM1 (6.82 mg/plant). The lowest bacoside A level (0.06 mg/plant) was recorded in accession BM14 during winter. Principal component analysis showed that samples of summer were positively correlated with both the components suggesting an appropriate time for the harvest.

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Antimicrobials from medicinal plants

Pandey¹,

Agnihotri

2015

Biotechnology of Bioactive Compounds

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Diversity among wild accessions of Bacopa monnieri (L.) Wettst. and their morphogenetic potential

Cited by 19 publications

References 37 publications

Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’ production from Bacopa monnieri (L.) Wettst.

Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’ production from Bacopa monnieri (L.) Wettst.

Seasonal variations in harvest index and bacoside A contents amongst accessions of Bacopa monnieri (L.) Wettst. collected from wild populations

Antimicrobials from medicinal plants

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