Diversity analysis of aflatoxigenic isolates of Aspergillus sp. from major groundnut growing states of India

Belludi, Rakesh; Sandhu, P.; Sharma, Pankaj; Sekhon, Amritpal Singh

doi:10.1007/s42360-022-00492-x

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…The results obtained from all nine isolates in the dendrogram were grouped into four main clusters (A, B, C, D). This can be explained by the high genetic variation observed among the isolates of Trichoderma combined with the fact that many of these isolates are the result of mutagenesis by ultraviolet light [27]. Consequently, cluster analysis performed by Jaccard showed that the isolates of T. atroviride were closely related to each other, and the fourth isolate of T. atroviride formed a cluster along with Hypocrea lixii, T. gamsii isolates, and two strains of T. longibrachiatum.…”

Section: Resultsmentioning

confidence: 99%

Genetic Diversity of Trichoderma and Hypocrea lixii Iraqi Strains by Using Random Amplified Polymorphic DNA (RAPD) Technique

Handel

2023

Iraqi Journal of Science

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As the diversity and characteristics of Trichoderma species are difficult to determine using morphological methods, henceforth molecular tools are crucial. This study utilized random amplified polymorphic DNA (RAPD) technique to investigate the genetic diversity of Trichoderma with sexual phase Hypocrea and to identify similarities and differences in the phylogenetic tree. Nine Iraqi Trichoderma strains (four strains of T. atroviride, one strain of Hypocrea lixii, two strains of T. gamsii and two strains of T. longibriantium) were examined in this research. The genomic DNA of each species was extracted and amplified with each of the five primers. 197 bands were obtained by using five oligodeoxynucleotide primers of which 98.47% were polymorphic and about 1.52% were monomorphic. When primers OPA4, OPA8, and OPA10 were used, the genetic variability was about 100%. Whereas, after using primers OPA7 and OPA5, the obtained genetic variability was 95.7% and 92.6% respectively. Gel images of the RAPD were processed with photo-cap program which detected the bands of isolates based on the ladder. The detected bands were then clustered based on the Jaccard method in Past software that showed T. atroviride, T. gamsii, Hypocrea lixii, and T. longibrachiatum isolates were grouped as clades and lineages. Although all strains belonged to the same species and group in one clade, they differed in size and number of bands. The Jaccard cluster analysis showed that the three isolates of T. atroviride were closely related to each other, while the four isolates of T. atroviride in one cluster were same as Hypocrea lixii, the isolates of T. gamsii and two strains of T. longibrachiatum formed one cluster. Thus, the high reliability of RAPD markers could be applied to identify Trichoderma species and create genetic maps instead of other DNA-based methods which are not only costly but time-consuming too.

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Section: Resultsmentioning

confidence: 99%

Genetic Diversity of Trichoderma and Hypocrea lixii Iraqi Strains by Using Random Amplified Polymorphic DNA (RAPD) Technique

Handel

2023

Iraqi Journal of Science

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“…The fungal color changes from transparent white to red, indicating that the isolate can produce aflatoxins. (Saito & Machida, 1999); (Belludi et al, 2022).…”

Section: Examine Some Fungal Isolates' Ability To Produce Aflatoxins ...mentioning

confidence: 99%

Isolation and identification of the most important aflatoxin B1-producing species in Najaf Governorate respiratory patients

AL-Masari

Al-Obaidi

2022

ijhs

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The purpose of this study was to evaluate the efficacy of some biological methods for removing toxins or reducing the toxic effect of aflatoxin. Initially, the genus Aspergillus spp was isolated from chronic lung infections, Sputum samples were collected from patients with chest and respiratory diseases who visited the consulting clinic, and the samples were subjected to direct microscopic examination and agricultural and molecular diagnosis using PCR technology. Aflatoxin B1 chemical detection also indicated the use of some poison detection methods, such as the ammonia vapour method, a kind-layer chromatic analysis, and high performance chromatography. The tested isolation gave the same result as the ammonia test for ultraviolet rays by changing the color of the inverted dish to red-carbon and expressing one isolation as a negative result of the test.

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“…Thus, the negative effect of these secondary metabolites on grain quality has been described [2]. The group of such secondary metabolites includes about 20 chemically related metabolites, some of them are identified as B1, B2, G1 and G2 [2,3].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity of Gram-Negative Pigment-Forming Bacteria Against Aspergillus Unguis

et al. 2023

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The paper presents a study of the antifungal activity of the pigment-forming strains Janthinobacterium lividum VKM B-3515, Pseudomonas chlororaphis VKM B-3546D and their mixture against the test culture Aspergillus unguis VKM F-1754. The growth rate of the colony and the average growth of A. unguis VKM F-1754 in the presence of J. lividum VKM B-3515 were 46.3% lower than the control variant. The growth rate of fungi in the presence of a mixture of two bacteria was 32% lower than in the control, and in the variant with a pure culture of Pseudomonas, it was 21%. The average increase in the diameter of the colonies of A. unguis VKM F-1754 cultivated with (P. chlororaphis VKM B-3546D-J. lividum VKM B-3515) was 33% lower than the control variant with Pseudomonas. While the mixture (P. chlororaphis VKM B-3546D-J. lividum VKM B-3515) showed less activity compared to the pure culture of J. lividum VKM B-3515. Thus, the use of two strains together may not be appropriate.

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Diversity analysis of aflatoxigenic isolates of Aspergillus sp. from major groundnut growing states of India

Cited by 4 publications

References 18 publications

Genetic Diversity of Trichoderma and Hypocrea lixii Iraqi Strains by Using Random Amplified Polymorphic DNA (RAPD) Technique

Genetic Diversity of Trichoderma and Hypocrea lixii Iraqi Strains by Using Random Amplified Polymorphic DNA (RAPD) Technique

Isolation and identification of the most important aflatoxin B1-producing species in Najaf Governorate respiratory patients

Antifungal Activity of Gram-Negative Pigment-Forming Bacteria Against Aspergillus Unguis

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