Diversity analysis of green gram (Vigna radiata (L.) Wilczek)through morphological and molecular markers

Kaur, Gunjeet; Joshi, Arunabh; Jain, Devendra; Choudhary, Ravish; Vyas, Divya

doi:10.3906/tar-1508-59

Cited by 16 publications

(9 citation statements)

References 17 publications

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“…The number of alleles (23) generated by the ten SSR markers analysis were shared among the studied varieties. The CEDG050 marker recorded the highest number of polymorphic alleles (5).…”

Section: Discussionmentioning

confidence: 97%

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Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Mwangi

Okoth

Kariuki

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Mung bean is a pulse crop principally grown in the tropic and subtropic parts of the world for its nutrient-rich seeds. Seven mung beans accessions from Eastern Kenya were evaluated using thirteen phenotypic traits. In addition, 10 SSR markers were used to determine their genetic diversity and population structure. This aimed at enhancing germplasm utilization for subsequent mung bean breeding programs. Results Analysis of variance for most of the phenology traits showed significant variation, with the yield traits recording the highest. The first three principal components (PC) explained 83.4% of the overall phenotypic variation, with the highest (PC1) being due to variation of majority of the traits studied such as pod length, plant height, and seeds per pod. The dendogram revealed that the improved genotypes had common ancestry with the local landraces. The seven mung beans were also genotyped using 10 microsatellite markers, eight of which showed clear and consistent amplification profiles with scorable polymorphisms in all the studied genotypes. Genetic diversity, allele number, and polymorphic information content (PIC) were determined using powermarker (version 3.25) and phylogenetic tree constructed using DARWIN version 6.0.12. Analysis of molecular variance (AMOVA) was calculated using GenALEx version 6.5. A total of 23 alleles were detected from the seven genotypes on all the chromosomes studied with an average of 2.875 across the loci. The PIC values ranged from 0.1224 (CEDG056) to 0.5918 (CEDG092) with a mean of 0.3724. Among the markers, CEDG092 was highly informative while the rest were reasonably informative except CEDG056, which was less informative. Gene diversity ranged from 0.1836 (CEDG050) to 0.5102 (CDED088) with an average of 0.3534. The Jaccards dissimilarity matrix indicated that genotypes VC614850 and N26 had the highest level of dissimilarity while VC637245 and N26 had lowest dissimilarity index. The phylogenetic tree grouped the genotypes into three clusters as revealed by population structure analysis (K = 3), with cluster III having one unique genotype (VC6137B) only. AMOVA indicated that the highest variation (99%) was between individual genotype. In addition, marker traits association analysis revealed 18 significant associations (P < 0.05). Conclusion These findings indicate sufficient variation among the studied genotypes that can be considered for germplasm breeding programs.

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“…The number of alleles (23) generated by the ten SSR markers analysis were shared among the studied varieties. The CEDG050 marker recorded the highest number of polymorphic alleles (5).…”

Section: Discussionmentioning

confidence: 97%

“…It was then diluted to 20 ng/μl and stored at −20 °C until use. Ten sets of simple sequence repeat (SSR) marker primers developed by Gunjeet [23] were used to amplify the extracted DNA. The primers were selected based on their annealing temperature and the amplicon size of at most 300 base pairs (Table 1).…”

Section: Molecular Characterizationmentioning

confidence: 99%

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Mwangi

Okoth

Kariuki

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…Assessment of genetic diversity in the varieties is of immense importance for planning an effective and scientific breeding program for enhancement of crop yield as well as its stability (Chandra et al 2013;Kaur et al 2016). For assessing genetic diversity, molecular markers in addition to the conventional morphological and biochemical markers are powerful tools since they remain unaffected by environmental factors and are independent of tissue or developmental stage of crops (Zhang et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of polymorphic simple sequence repeats markers for the analysis of genetic diversity in Indian mungbean [Vigna radiata (L.) Wilczek]

Bangar¹,

Chaudhury²,

Umdale³

et al. 2017

Ind. Jrnl. Gen. Plnt. Bree.

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Mungbean is a widely cultivated grain legume in Asia, Africa and South America. In this study, 52 varieties of mungbean were profiled with 39 polymorphic SSR primers after screening a total 315 SSR primers. A total of 96 alleles were scored from the 39 primers with an average of 2.46 alleles per locus indicating the low diversity among varieties. The Nei's genetic diversity index and the Shannon information index of SSR primers varied from 0 to 0.649 and 0 to 1.169, respectively. The results showed that the potential transferability of adzuki bean primers (83.3%) was greater than the cowpea primers (25%). The varieties profiled were grouped into four major clusters. But the clustering pattern did not reflect on their geographical origin. Further, the AMOVA indicated presence of moderate genetic differentiation among groups compared to higher differentiation among varieties within populations. The SSR markers identified here will add valuable genomic resources for germplasm characterization, cultivar identification and assessment of genetic diversity of mungbean varieties.

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“…components. Kaur et al (2016) reported the UPGMA dendrogram based on the combined morphological and molecular markers in which the 23 mungbean genotypes were divided into three main clusters, showing a close genetic relationship, which might be due to their close genetic bases [35].…”

Section: Genetic Diversity In Plant Species -Characterization and Conmentioning

confidence: 99%

Morphological, Biochemical, and Molecular Characterization of Orange-Fleshed Sweet Potato (Ipomoea batatas [L.] Lam) Germplasms

Sharma¹,

Jain²,

Khandelwal³

et al. 2019

Genetic Diversity in Plant Species - Characterization and Conservation

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The sweet potato is considered as an excellent source of β-carotene and anthocyanins and has a considerable value in the functional food market. In this report, 21 sweet potato (Ipomoea batatas [L.] Lam) germplasms were evaluated for genetic diversity using morphological and biochemical and molecular markers. Ten morphological traits were studied, and the mean squares due to germplasm were highly significant for storage root number per plant, individual root weight, storage root (fresh) per plant, storage root (dry) per plant, storage root yield, and storage root length. UPGMA cluster analysis based on morphological traits separated the germplasm into three groups. The similarity coefficient ranged from 0.00 to 0.50 with an average of 0.176. Biochemical analysis, viz. total phenol and antioxidant, was performed to find out superior genotype at biochemical level under given conditions. Maximum total phenol was observed in the genotype "V-12" (1.39 mg), whereas maximum total antioxidant was observed in "Samrat" (0.30 mg). RAPD analysis was carried out, and out of 15 RAPD primers, 10 primers produced 96 reproducible and polymorphic bands. UPGMA cluster analysis based on RAPD data also separated the genotypes into three clusters. The results of the present study can be used for sweet potato crop improvement through molecular breeding and marker-assisted selection for desired traits in future.

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Diversity analysis of green gram (Vigna radiata (L.) Wilczek)through morphological and molecular markers

Cited by 16 publications

References 17 publications

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Genetic and phenotypic diversity of selected Kenyan mung bean (Vigna radiata L. Wilckzek) genotypes

Detection and characterization of polymorphic simple sequence repeats markers for the analysis of genetic diversity in Indian mungbean [Vigna radiata (L.) Wilczek]

Morphological, Biochemical, and Molecular Characterization of Orange-Fleshed Sweet Potato (Ipomoea batatas [L.] Lam) Germplasms

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