Diversity and antibiotic resistance profiles of Pseudomonads from a hospital wastewater treatment plant

Santoro, D.O.; Cardoso, Alexander Machado; Coutinho, Felipe H.; Pinto, Leonardo Henriques; Vieira, Ricardo P.; Albano, Rodolpho Mattos; Clementino, Maysa M.

doi:10.1111/jam.12936

Cited by 38 publications

(22 citation statements)

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“…Resistance was also noted to have emerged in some environmental lineages against various antibiotics, for instance, ertapenem, cefoxitin, amikacin, and amoxicillin-clavulanic acid in sewage water isolates (Additional file 2: Figure S3), which presumably arises in response to antibiotics in environmental wastewater, as reported previously [42, 43]. Several studies have shown the presence of highly antibiotic-resistant bacteria and resistance genes in sewage released into aquatic environments [42, 44–46]. In line with these findings, our results suggest that the release of untreated hospital sewage may play a role in the environmental emergence and spread of multiresistant pathogenic bacteria, and that wastewater (including hospital waste) warrants treatment to eliminate these organisms prior to release.…”

Section: Discussionsupporting

confidence: 53%

Whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental Klebsiella pneumoniae

et al. 2017

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Background Klebsiella pneumoniae is a gram-negative bacterial species capable of occupying a broad range of environmental and clinical habitats. Known as an opportunistic pathogen, it has recently become a major causative agent of clinical infections worldwide. Despite growing knowledge about the highly diverse population of K. pneumoniae, the evolution and clinical significance of environmental K. pneumoniae, as well as the relationship between clinical and environmental K. pneumoniae, are poorly defined.MethodsWe isolated and sequenced K. pneumoniae from in-patients in a single hospital in Thailand, as well as hospital sewage, and surrounding canals and farms within a 20-km radius.ResultsPhylogenetic analysis of 77 K. pneumoniae (48 clinical and 29 non-clinical isolates) demonstrated that the two groups were intermixed throughout the tree and in some cases resided in the same clade, suggesting recent divergence from a common ancestor. Phylogenetic comparison of the 77 Thai genomes with 286 K. pneumoniae from a global collection showed that Thai isolates were closely related to the clinical sub-population of the global collection, indicating that Thai clinical isolates belonged to globally circulating lineages. Dating of four Thai K. pneumoniae clades indicated that they emerged between 50 and 150 years ago. Despite their phylogenetic relatedness, virulence factors and β-lactamase resistance genes were more numerous in clinical than in environmental isolates. Our results indicate that clinical and environmental K. pneumoniae are closely related, but that hospitals may select for isolates with a more resistant and virulent genotype.ConclusionsThese findings highlight the clinical relevance of environmental K. pneumoniae isolates.Electronic supplementary materialThe online version of this article (doi:10.1186/s13073-017-0397-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 53%

Whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental Klebsiella pneumoniae

et al. 2017

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“…The role of hospitals in the environmental release of antibiotic-resistant bacteria and ARGs has been demonstrated and has become a growing concern for public health [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. Hospital WWTPs can harbour antibiotic-resistant bacteria and ARGs [ 48 , 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Antibiotic Resistance Genes in Escherichia coli Isolates from Hospital Wastewater in Vietnam

Lien

Lan

Chúc

et al. 2017

IJERPH

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The environmental spread of antibiotic-resistant bacteria has been recognised as a growing public health threat for which hospitals play a significant role. The aims of this study were to investigate the prevalence of antibiotic resistance and antibiotic resistance genes (ARGs) in Escherichia coli isolates from hospital wastewater in Vietnam. Wastewater samples before and after treatment were collected using continuous sampling every month over a year. Standard disk diffusion and E-test were used for antibiotic susceptibility testing. Extended-spectrum beta-lactamase (ESBL) production was tested using combined disk diffusion. ARGs were detected by polymerase chain reactions. Resistance to at least one antibiotic was detected in 83% of isolates; multidrug resistance was found in 32%. The highest resistance prevalence was found for co-trimoxazole (70%) and the lowest for imipenem (1%). Forty-three percent of isolates were ESBL-producing, with the blaTEM gene being more common than blaCTX-M. Co-harbouring of the blaCTX-M, blaTEM and qepA genes was found in 46% of isolates resistant to ciprofloxacin. The large presence of antibiotic-resistant E. coli isolates combined with ARGs in hospital wastewater, even post-treatment, poses a threat to public health. It highlights the need to develop effective processes for hospital wastewater treatment plants to eliminate antibiotic resistant bacteria and ARGs.

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“…In terms of culture-based techniques, some consensus is emerging around E. coli as a highly suitable target (Blaak et al, 2015b;Liang et al, 2015), although many other potentially useful bacterial targets, such as Klebsiella spp. (Berendonk et al, 2015), fecal enterococci (Berendonk et al, 2015), and bacteria that grow in aquatic/soil environments such as Pseudomonas aeruginosa (Santoro et al, 2015) or various Aeromonads exist. However, E. coli is a practical choice given that it is already the most widely monitored target as an indicator of fecal pollution and thus methodologies are already standardized and infrastructure is more likely to be in place to implement monitoring campaigns based on E. coli (Matheu et al, 2017).…”

Section: Detection Methods For Antibiotic Resistance Monitoring Targetsmentioning

confidence: 99%

Antimicrobal Resistance: Fecal Sanitation Strategies for Combatting a Global Public Health Threat

Ashbolt¹,

Pruden²,

Miller³

et al. 2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Diversity and antibiotic resistance profiles of Pseudomonads from a hospital wastewater treatment plant

Abstract: The presence of MDR bacteria from treated effluents alerts for the need to improve these systems to avoid the spreading of resistance genes in aquatic ecosystems. This has special relevance in Brazil, where a significant portion of the population has no access to treated water.

Cited by 38 publications

References 72 publications

Whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental Klebsiella pneumoniae

Whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental Klebsiella pneumoniae

Antibiotic Resistance and Antibiotic Resistance Genes in Escherichia coli Isolates from Hospital Wastewater in Vietnam

Antimicrobal Resistance: Fecal Sanitation Strategies for Combatting a Global Public Health Threat

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