Diversity and antimicrobial potential of culturable heterotrophic bacteria associated with the endemic marine sponge<i>Arenosclera brasiliensis</i>

Rua, Cintia P. J.; Trindade-Silva, Amaro E.; Appolinario, Luciana; Venas, Tainá Moreira Martins; Garcia, Gizele D.; Carvalho, Lucas Silva; Lima, A. T. M.; Krüger, Ricardo Henrique; Pereira, Renato Crespo; Berlinck, Roberto G. S.; Valle, Rogério; Thompson, Cristiane C.; Thompson, Fabiano L.

doi:10.7717/peerj.419

Cited by 75 publications

(70 citation statements)

References 59 publications

(74 reference statements)

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“…are found to comprise 70 to 95% of the microbiome in several Red Sea (Stylophora pistillata, Pocillopora damicornis, and Acropora humilis) and Caribbean corals (Porites astreoides [49][50][51][52]). These bacteria have also been shown to live endosymbiotically, forming large aggregations deep within coral tissues (51), potentially playing a role in bacterial communication and structuring via the production of quorum-sensing signaling metabolites and antimicrobial compounds (53). Recently, Endozoicomonas species specificity was shown to map onto the different reproductive strategies of the coral host, suggesting that symbiont selection may be linked to life history (54).…”

Section: Discussionmentioning

confidence: 99%

Allelochemicals Produced by Brown Macroalgae of the Lobophora Genus Are Active against Coral Larvae and Associated Bacteria, Supporting Pathogenic Shifts to Vibrio Dominance

Morrow

Bromhall

Motti

et al. 2017

Appl Environ Microbiol

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Pervasive environmental stressors on coral reefs are attributed with shifting the competitive balance in favor of alternative dominants, such as macroalgae. Previous studies have demonstrated that macroalgae compete with corals via a number of mechanisms, including the production of potent primary and secondary metabolites that can influence coral-associated microbial communities. The present study investigates the effects of the Pacific brown macroalga Lobophora sp. (due to the shifting nature of the Lobophora species complex, it will be referred to here as Lobophora sp.) on coral bacterial isolates, coral larvae, and the microbiome associated with the coral Porites cylindrica. Crude aqueous and organic macroalgal extracts were found to inhibit the growth of coral-associated bacteria. Extracts and fractions were also shown to inhibit coral larval settlement and cause mortality at concentrations lower (Ͻ0.3 mg · ml Ϫ1 ) than calculated natural concentrations (4.4 mg · ml Ϫ1 ). Microbial communities associated with coral tissues exposed to aqueous (e.g., hydrophilic) crude extracts demonstrated a significant shift to Vibrio dominance and a loss of sequences related to the putative coral bacterial symbiont, Endozoicomonas sp., based on 16S rRNA amplicon sequencing. This study contributes to growing evidence that macroalgal allelochemicals, dissolved organic material, and native macroalgal microbial assemblages all play a role in shifting the microbial equilibrium of the coral holobiont away from a beneficial state, contributing to a decline in coral fitness and a shift in ecosystem structure.IMPORTANCE Diverse microbial communities associate with coral tissues and mucus, providing important protective and nutritional services, but once disturbed, the microbial equilibrium may shift from a beneficial state to one that is detrimental or pathogenic. Macroalgae (e.g., seaweeds) can physically and chemically interact with corals, causing abrasion, bleaching, and overall stress. This study contributes to a growing body of evidence suggesting that macroalgae play a critical role in shifting the coral holobiont equilibrium, which may promote the invasion of opportunistic pathogens and cause coral mortality, facilitating additional macroalgal growth and invasion in the reef. Thus, macroalgae not only contribute to a decline in coral fitness but also influence coral reef ecosystem structure.KEYWORDS DNA sequencing, Lobophora, Porites, QIIME, coral larvae, coral reef, holobiont, macroalgae, metagenomics, pathogens

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Section: Discussionmentioning

confidence: 99%

Allelochemicals Produced by Brown Macroalgae of the Lobophora Genus Are Active against Coral Larvae and Associated Bacteria, Supporting Pathogenic Shifts to Vibrio Dominance

Morrow

Bromhall

Motti

et al. 2017

Appl Environ Microbiol

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“…With this study we could furthermore add aeroplysinin-1 to the list of antibiotic resistances exhibited by Pseudovibrio species (Versluis et al, 2017). The genus Ruegeria is part of the abundant seawaterdwelling Roseobacter lineage (Buchan et al, 2005;Wagner-Döbler and Biebl, 2006) and has frequently been isolated from sponges and other marine environments (Mitova et al, 2004;Muscholl-Silberhorn et al, 2008;Esteves et al, 2013;Rua et al, 2014). Two other shared OTUs (OTU-168, 169) belong to the class Flavobacteriia, members of which are regularly detected in and isolated from marine sponges, however, their roles as sponge symbionts remain to be investigated (Lavy et al, 2014;Montalvo et al, 2014;Horn et al, 2016;Yoon et al, 2016;Versluis et al, 2017).…”

Section: Most Cultivated Taxa Differ From Sponge-associated Bacteriamentioning

confidence: 99%

Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients

et al. 2020

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Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, "omics"-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cultivate bacteria derived from the marine sponge Aplysina aerophoba, nine novel media formulations were created based on the predicted genomic potential of the prevalent sponge symbiont lineage Poribacteria. In addition, to maintain potential microbial metabolic interactions in vitro, a Liquid-Solid cultivation approach and a Winogradsky-column approach were applied. The vast majority of microorganisms in the inoculum appeared viable after cryopreservation of sponge specimen as determined by selective propidium monoazide DNA modification of membrane-compromised cells, however, only 2% of the initial prokaryotic diversity could be recovered through cultivation. In total, 256 OTUs encompassing seven prokaryotic phyla were cultivated. The diversity of the cultivated community was influenced by the addition of the antibiotic aeroplysinin-1 as well as by medium dilution, rather than carbon source. Furthermore, the Winogradsky-column approach reproducibly enriched distinct communities at different column depths, amongst which were numerous Clostridia and OTUs that could not be assigned to a known phylum. While some bacterial taxa such as Pseudovibrio and Ruegeria were recovered from nearly all applied cultivation conditions, others such as Bacteroidetes were specific to certain medium types. Predominant sponge-associated prokaryotic taxa remained uncultured, nonetheless, alternative cultivation approaches applied here enriched for previously uncultivated microbes.

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“…Symbiotic marine bacteria chemically protect their host organisms from pathogenic organisms [1]. Additionally, symbiotic bacteria are known to have various roles, such as host health, nutrition, and antibiotic production for host organisms [2][3][4][5][6]. Over the past 85 years, about 50,000 natural products have been discovered from microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance of Symbiotic Marine Bacteria Isolated from Marine Organisms in Jeju Island of South Korea

Park¹,

Lee²,

Lee³

et al. 2018

J Oceanogr Mar Res

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Diversity and antimicrobial potential of culturable heterotrophic bacteria associated with the endemic marine spongeArenosclera brasiliensis

Cited by 75 publications

References 59 publications

Allelochemicals Produced by Brown Macroalgae of the Lobophora Genus Are Active against Coral Larvae and Associated Bacteria, Supporting Pathogenic Shifts to Vibrio Dominance

Allelochemicals Produced by Brown Macroalgae of the Lobophora Genus Are Active against Coral Larvae and Associated Bacteria, Supporting Pathogenic Shifts to Vibrio Dominance

Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients

Antibiotic Resistance of Symbiotic Marine Bacteria Isolated from Marine Organisms in Jeju Island of South Korea

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