Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant &amp;lt;i&amp;gt;Echinacea purpurea&amp;lt;/i&amp;gt; (L.) Moench

Rosa, Luiz Henrique; Tabanca, Nurhayat; Techen, Natascha; Wedge, David E.; Pan, Zhiqiang; Bernier, Ulrich R.; Becnel, James J.; Agramonte, Natasha M.; Walker, Larry; Moraes, Rita M.

doi:10.4236/ajps.2012.38133

Cited by 28 publications

(17 citation statements)

References 28 publications

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“…However, Ravnikar et al have reported the dichloromethane extract of P. herbarum to possess better antimicrobial activity than ethyl acetate extract [70]. Our results differ from the findings of Rosa et al who have described F. solani as ineffective against C. gloeosporioides [71]. The plausible reason for this dissimilarity might be the difference in the strain of fungi and also because of varied solvents used in both the studies for obtaining fungal extracts.…”

Section: Evaluation Of Endophytic Fungal Isolates and Crude Fungal Excontrasting

confidence: 85%

“…The plausible reason for this dissimilarity might be the difference in the strain of fungi and also because of varied solvents used in both the studies for obtaining fungal extracts. It suggests the efficiency of ethyl acetate extract over ethanolic extract used by Rosa et al [71]. Isolation and Characterization of Compound from P. herbarum…”

Section: Evaluation Of Endophytic Fungal Isolates and Crude Fungal Exmentioning

confidence: 98%

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Production of Gentisyl Alcohol from Phoma herbarum Endophytic in Curcuma longa L. and Its Antagonistic Activity Towards Leaf Spot Pathogen Colletotrichum gloeosporioides

Gupta

Kaul

Singh

et al. 2016

Appl Biochem Biotechnol

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Endophytes from medicinal plants represent a potential source of bioactive compounds. During the present investigation, fungal endophytes were isolated from turmeric (Curcuma longa), an important medicinal plant. A total of 207 endophytic fungal isolates were obtained from the rhizome of C. longa L. They were grouped into seven genera based on morphological and molecular data. The fungal endophytes of C. longa were evaluated for antifungal activity against Colletotrichum gloeosporioides, the causal organism of leaf spot of turmeric. The disease is a major cause for economic loss in turmeric cultivation. Endophytic Phoma herbarum showed significant activity against C. gloeosporioides and was therefore selected for further studies. A compound gentisyl alcohol was isolated from P. herbarum which showed effective antagonism against C. gloeosporioides. The organism could therefore be used as a biocontrol agent against C. gloeosporioides.

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Section: Evaluation Of Endophytic Fungal Isolates and Crude Fungal Excontrasting

confidence: 85%

Section: Evaluation Of Endophytic Fungal Isolates and Crude Fungal Exmentioning

confidence: 98%

Production of Gentisyl Alcohol from Phoma herbarum Endophytic in Curcuma longa L. and Its Antagonistic Activity Towards Leaf Spot Pathogen Colletotrichum gloeosporioides

Gupta

Kaul

Singh

et al. 2016

Appl Biochem Biotechnol

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“…The genus Mycoleptodiscus comprises 15 species, including endophytes, plant pathogens, and human pathogens (Padhye et al, 1995;Watanabe et al, 1997;Rosa et al, 2012). Although the extract of M. indicus was found to exhibit larvicidal activity against A. aegypti, relatively little is known about the antifungal activity of this species.…”

Section: Discussionmentioning

confidence: 99%

A cultured endophyte community is associated with the plant Clerodendrum inerme and antifungal activity

Gong¹,

Xh²,

Yq³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Fungal endophytes live in the inner tissues of Clerodendrum inerme and may be significant resources for new chemicals in drug discovery. A total of 242 endophytic fungi were recovered from 602 sample segments of C. inerme; 66 were purified. The 66 fungi belonging to 16 taxa and 11 genera (Alternaria, Nigrospora, Bartalinia, Pestalotiopsis, Fusarium, Mycoleptodiscus, Trichoderma, Phomopsis, Diaporthe, Lasiodiplodia, and Curvularia) were identified by morphological characteristics and fungal internal transcribed spacer sequences. The most abundant genera were Alternaria and Lasiodiplodia. Some of the endophytes exhibited tissue specificity. The colonization frequencies of endophytes in the stems were evidently higher than those in the roots and leaves. The crude ethyl acetate extracts were tested against 6 endophytes isolated from C. inerme. Three of 10 (33.3%) endophytes, which were identified as Phomopsis sp, Curvularia sp, and Mycoleptodiscus sp, displayed distinct antifungal activity against ≥3 tested fungi. To our knowledge, this is the first report of an endophytic community associated with C. inerme in China and its antifungal activity in vitro.

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“…The extracts were obtained by maceration of the mycelium in ethyl acetate (EtOAc), according to Rosa et al (2012) with modifications. Endophytic strains were grown in Petri dishes containing about 20 mL of PDA maintained at 28°C.…”

Section: Preparation Of Endophytic Fungi Extractsmentioning

confidence: 99%

Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler

et al. 2016

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We report the isolation and identification of endophytic fungi from Combretum lanceolatum Pohl ex Eichler. Further, we evaluated the relationships of fungi with the host plant and tested bioactivities of isolates. The fungi were isolated from disinfected root fragments and plated onto potato dextrose agar. Root pieces were also used to quantify fungal structures associated with the roots. Identification of fungi was carried out by characterization of morphological features and sequencing of the ITS region. Endophytism was confirmed by inoculation of endophyte-free seedlings followed by microscopic examination. The extract was obtained by maceration of the mycelium in ethyl acetate for antioxidant and antimicrobial evaluations. A total of 112 strains belonging to nine different species were isolated, the major classes were Dothideomycetes and Sordariomycetes. C. lanceolatum is colonized by dark septate endophytes (DSE), evidenced by the presence of microsclerotia and melanized hyphae. There is also co-colonization with mycorrhizal fungi in the same root fragments. Seedling inoculation experiments revealed that C. perangustum-95C and M. phaseolina-46C showed association with the seedlings of C. lanceolatum and differentiated microsclerotia and dark septate hyphae, indicating that these species are DSE. In the antimicrobial test, the D. phaseolorum-92C extract had the highest zones of inhibition against S. aureus and E. coli, respectively. The results showed that 100 % of the extracts have antioxidant activity ranging from low to moderate. All endophyte species had antioxidant and antimicrobial activities that were directly proportional to the dose-responses. Future research will involve chemical characterization and structural elucidation of bioactive compounds.

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Diversity and Biological Activities of Endophytic Fungi Associated with Micropropagated Medicinal Plant <i>Echinacea purpurea</i> (L.) Moench

Cited by 28 publications

References 28 publications

Production of Gentisyl Alcohol from Phoma herbarum Endophytic in Curcuma longa L. and Its Antagonistic Activity Towards Leaf Spot Pathogen Colletotrichum gloeosporioides

Production of Gentisyl Alcohol from Phoma herbarum Endophytic in Curcuma longa L. and Its Antagonistic Activity Towards Leaf Spot Pathogen Colletotrichum gloeosporioides

A cultured endophyte community is associated with the plant Clerodendrum inerme and antifungal activity

Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler

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