Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria

Lazarkevich, Irina; Tomova, Iva; Kambourova, Margarita; Vasileva−Tonkova, Evgenia

doi:10.5038/1827-806x.42.1.8

Cited by 63 publications

(39 citation statements)

References 47 publications

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“…The input of microorganisms by dripping water and periodic flooding of the river may carry organisms that cannot survive the cave conditions and are used by opportunistic fungi as a source of nutrients. Use of a nutrient-rich medium for the isolation of fungi may have restricted the growth of strains that are better adapted to oligotrophic conditions (Tomova et al, 2013). We observed that the number of isolates was higher in the samples deeper inside the cave compared to the entrance zone.…”

Section: Discussionsupporting

confidence: 53%

Terrestrial filamentous fungi from Gruta do Catão (São Desidério, Bahia, Northeastern Brazil) show high levels of cellulose degradation

Paula¹,

Montoya²,

Rodrigues³

et al. 2016

JCKS

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Subterranean environments are oligotrophic. However, few studies have investigated the composition and function of their terrestrial mycobiota. This study examined the functional role of filamentous cave fungi in cellulose degradation. Soil samples and dry sediment were collected in the surface epigean environment and two sites in the entrance and twilight zones inside Gruta do Catão in the São Desidério karst area, state of Bahia, Brazil. Fungi were cultured from the samples, and the total organic carbon, culturable microorganisms, and carbon and nitrogen microbial biomasses were estimated. All fungal strains were evaluated for cellulase production in carboxymethylcellulose synthetic medium, and the enzymatic indices were estimated. We observed a significant difference (p 0.05) in physical, chemical, and biological parameters between epigean soil and cave sediments by Tukey's test. We recovered a total of 20 isolates comprising the genera Aspergillus (50.0%), Penicillium (25.0%), Talaromyces (10.0%), Trichoderma (5.0%), Purpureocillium (5.0%) and Scopulariopsis (5.0%). The majority of the isolates (90%) showed cellulolytic activity, which is a higher percentage compared to that normally reported in the literature for sediments. Thus there is a high probability that the filamentous fungi act in nutrient cycling, thereby contributing to the quality and maintenance of the cave ecosystem. These results indicate that parameters such as total organic carbon, biomass, and relative humidity that tend to differ between caves and epigean environments, provide selective pressures for microorganisms that use alternative sources of energy and nutrients.

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Section: Discussionsupporting

confidence: 53%

Terrestrial filamentous fungi from Gruta do Catão (São Desidério, Bahia, Northeastern Brazil) show high levels of cellulose degradation

Paula¹,

Montoya²,

Rodrigues³

et al. 2016

JCKS

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show abstract

“…intermedius, E. coli, Klebsiella ozaenae, K. oxytoca, K. pneumoniae and Pantoea agglomerans in cave pools. In some studies, predominance of Serratia isolates was confirmed in the karst waters probably due to insects and bats faeces (Janda 2001; Kämpfer et al 2008;Jurado et al 2010;Tomova et al 2013).…”

Section: Discussionmentioning

confidence: 95%

The occurrence of coliform bacteria in the cave waters of Slovak Karst, Slovakia

Seman

Gaálová

Cíchová³

et al. 2014

Folia Microbiol

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The diversity and abundance of coliform bacteria (taxonomically enterobacterias), an important quality water indicator, were determined for four representative caves in Slovak Karst: Domica Cave, Gombasecká Cave, Milada Cave and Krásnohorská Cave. Three hundred and fifty-two enterobacterial isolates were successfully identified by biochemical testing (commercial ENTEROtest 24) and selected isolates confirmed by molecular techniques (PCR, 16S rDNA sequence analysis). A total of 39 enterobacterial species were isolated from cave waters, with predominance of Escherichia coli, Serratia spp. and Enterobacter spp. PCR amplification of lacZ gene is not specific enough to provide a reliable detection of coliform bacteria isolated from the environment. Sequence analysis of 16S rDNA confirmed that all of the selected isolates belong to the family Enterobacteriaceae. In general, physical and chemical parameters of cave waters in Slovak Karst corresponded to national drinking water quality standards.

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“…The 16S rRNA gene was amplified from the extracted DNA using universal bacterial prim− ers 8F and 1492R (Weisburg et al 1991). PCR was performed as described in our previous work (Tomova et al 2013). The obtained PCR products were purified and sequenced in Macrogen Inc. (South Korea).…”

Section: Methodsmentioning

confidence: 99%

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Tomova¹,

Stoilova−Disheva²,

Vasileva−Tonkova³

2014

Polish Polar Research

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Abstract:In this study, selected heavy metals resistant heterotrophic bacteria isolated from soil samples at the Windmill Islands region, Wilkes Land (East Antarctica), were character− ized. Phylogenetic analysis revealed affiliation of isolates to genera Bacillus, Lysini− bacillus, Micrococcus and Stenotrophomonas. The strains were found to be psychrotolerant and halotolerant, able to tolerate up to 10% NaCl in the growth medium. The Minimum In− hibitory Concentration of the seven heavy metals Cr, Cu, Ni, Co, Cd, Zn, and Pb was deter− mined in solid media for each bacterial strain. Gram−positive Vi−2 strain and Gram−negative Vi−4 strain showed highest multiply heavy metals resistance, and Vi−3 and Vi−4 strains showed multi−antibiotic resistance to more than a half of the 13 used antibiotics. Plasmids were detected only in Gram−negative Vi−4 strain. The bacteria were able to produce differ− ent hydrolytic enzymes including industrially important proteases, xylanases, cellulases, and b−glucosidases. High heavy metals resistance of the Antarctic bacteria suggests their potential application for wastewater treatment in cold and temperate climates. Highly sensi− tive to Cd and Co ions Vi−1, Vi−5 and Vi−7 strains would be promising for developing biosensors to detect these most toxic heavy metals in environmental samples.

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Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria

Cited by 63 publications

References 47 publications

Terrestrial filamentous fungi from Gruta do Catão (São Desidério, Bahia, Northeastern Brazil) show high levels of cellulose degradation

Terrestrial filamentous fungi from Gruta do Catão (São Desidério, Bahia, Northeastern Brazil) show high levels of cellulose degradation

The occurrence of coliform bacteria in the cave waters of Slovak Karst, Slovakia

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

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