Diversity and Biotechnological Potential of Marine Actinomycetes from India

Sarkar, Gargi; Suthindhiran, K.

doi:10.1007/s12088-022-01024-x

Cited by 27 publications

(17 citation statements)

References 48 publications

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“…In post-harvest fruit management, antagonists are subjected to changes in pH, temperature, and humidity because in these conditions the efficiency of biocontrol agents can be affected [ 99 ]. Actinomycetes from marine, saline, hypersaline, and wetland environments are subjected to extreme environmental conditions that allow them to adapt to the changes in temperature, pH, and humidity that occur post-harvest [ 19 , 100 ]. However, in most of the studies of actinomycetes and biocontrol of phytopathogenic fungi in post-harvest fruit, the isolates provided are from terrestrial environments ( Figure 4 ).…”

Section: Antifungal Activity Of Actinomycetes Isolated In Different E...mentioning

confidence: 99%

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Torres-Rodríguez¹,

Pérez

Quiñones-Aguilar³

et al. 2022

Plants

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Synthetic fungicides have been the main control of phytopathogenic fungi. However, they cause harm to humans, animals, and the environment, as well as generating resistance in phytopathogenic fungi. In the last few decades, the use of microorganisms as biocontrol agents of phytopathogenic fungi has been an alternative to synthetic fungicide application. Actinomycetes isolated from terrestrial, marine, wetland, saline, and endophyte environments have been used for phytopathogenic fungus biocontrol. At present, there is a need for searching new secondary compounds and metabolites of different isolation sources of actinomycetes; however, little information is available on those isolated from other environments as biocontrol agents in agriculture. Therefore, the objective of this review is to compare the antifungal activity and the main mechanisms of action in actinomycetes isolated from different environments and to describe recent achievements of their application in agriculture. Although actinomycetes have potential as biocontrol agents of phytopathogenic fungi, few studies of actinomycetes are available of those from marine, saline, and wetland environments, which have equal or greater potential as biocontrol agents than isolates of actinomycetes from terrestrial environments.

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Section: Antifungal Activity Of Actinomycetes Isolated In Different E...mentioning

confidence: 99%

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Torres-Rodríguez¹,

Pérez

Quiñones-Aguilar³

et al. 2022

Plants

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“…BCy which was isolated from Cymodocearotundata seagrass using two different media [18], Sarkar and Suthindhiran (2022) also suggested the diversity of marine actinobacteria from different marine ecosystems and found 30 types of actinobacteria from various types of seaweed such as Cymodaceae sp., Enhalus sp., Halophila sp., Halodule sp., Syringodium sp., and Thalassia sp. [19]. In addition, the diversity of heterotroph bacteria in seagrass ecosystems was also shown by Boontanom and Chantarasiri (2020), who found a diversity of 9 isolates of epiphytic bacteria isolated from seaweed (Halodule uninervis) [20].…”

Section: Morphological Characteristics Of Cellulolytic Bacterial Isol...mentioning

confidence: 78%

Cellulolytic activity at the low temperature of associative bacteria from a seagrass ecosystem

Simbolon

Ismet

Ismiati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Cellulase enzymes have been widely used in many industrial activities, such as the production of biofuels, biofertilizers, and bio-decomposers. The usage of cellulase enzymes on an industrial scale usually involves a relatively high temperature. Microorganisms in marine ecosystems are widely known to have the ability to produce cellulase enzymes in various environmental ranges. However, exploring marine cellulolytic bacteria at low temperatures still lacks information. This study aims to determine the cellulolytic activity of bacteria associated with seagrass ecosystems at low temperatures. Cellulolytic activity test used selected bacteria isolates from the seagrass ecosystem (actinobacteria: AA10b, AA11a, AA30a, AB22b; heterotrophic bacterial: HB1.1, HB2.1, HT1.2, HT2.2). The cellulolytic index values were obtained from the clear zone test on CMC 1% medium with congo red staining (actinobacteria) and iodine (heterotrophic bacteria) in different temperature treatments (4°C, 17°C, 20°C, and 40°C). The results showed that all isolates had various cellulolytic index values at different temperatures. There was no bacterial cellulolytic activity at 4°C. However some isolates showed activities at 17°C and increased as the temperature was higher. The highest production of cellulolytic activity occurred at 40°C for both actinobacteria and heterotrophic bacteria isolates. The actinobacteria isolate AA11a had the highest cellulolytic index of 7.00 at 40°C. This study indicates that at low temperatures, associative bacteria from the seagrass ecosystem have considerable cellulolytic activity for further research applications in various industries.

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“…Because nearly 80% of our collection was able to use CMC as only carbon source, we evaluated cellulose degradation in CCM1 agar medium in terms of enzymatic index (EI) to select the best possible cellulolytic candidate. Calculating EI is used to evaluate enzyme-producing prospects within the same genus ( Teather & Wood, 1982 ) and was previously used for this purpose in other studies ( Barzkar & Sohail, 2020 ; Fatokun, Nwodo & Okoh, 2016 ; Lewin et al., 2022 ; Sarkar & Suthindhiran, 2022 ). In this study, 12 out of 196 strains showed high EI values (≥ 3.0) and were considered as highly cellulolytic, so they were selected for further quantitative enzymatic assays ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Escudero-Agudelo,

Martínez-Villalobos,

Arocha-Garza

et al. 2023

PeerJ

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The quest for microbial cellulases has intensified as a response to global challenges in biofuel production. The efficient deconstruction of lignocellulosic biomass holds promise for generating valuable products in various industries such as food, textile, and detergents. This article presents a systematic bioprospection aimed at isolating actinomycetes with exceptional cellulose deconstruction capabilities. Our methodology explored the biodiverse oligotrophic region of Cuatro Cienegas, Coahuila, within the Chihuahuan Desert. Among the evaluated actinomycetes collection, 78% exhibited cellulolytic activity. Through a meticulous screening process based on enzymatic index evaluation, we identified a highly cellulolytic Streptomyces strain for further investigation. Submerged fermentation of this strain revealed an endoglucanase enzymatic activity of 149 U/mg. Genomic analysis of strain Streptomyces sp. STCH565-A revealed unique configurations of carbohydrate-active enzyme (CAZyme) genes, underscoring its potential for lignocellulosic bioconversion applications. These findings not only highlight the significance of the Chihuahuan Desert as a rich source of cellulolytic microorganisms but also offer insights into the systematic exploration and selection of high-performing cellulolytic microorganisms for application in diverse environmental contexts. In conclusion, our bioprospecting study lays a foundation for harnessing the cellulolytic potential of actinomycetes from the Chihuahuan Desert, with implications for advancing cellulose deconstruction processes in various industries. The findings can serve as a blueprint for future bioprospecting efforts in different regions, facilitating the targeted discovery of microorganisms with exceptional cellulosic deconstruction capabilities.

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Diversity and Biotechnological Potential of Marine Actinomycetes from India

Cited by 27 publications

References 48 publications

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Cellulolytic activity at the low temperature of associative bacteria from a seagrass ecosystem

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

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