Diversity and Co-Occurrence Patterns of Fungal and Bacterial Communities from Alkaline Sediments and Water of Julong High-Altitude Hot Springs at Tianchi Volcano, Northeast China

Wang, Xiao; Pecoraro, Vincent L.

doi:10.3390/biology10090894

Cited by 9 publications

(6 citation statements)

References 71 publications

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“…all had best hits that originated in Arctic sediments ( F. petrolei , Chaudhary et al, 2019 ) or thawing permafrost samples ( F. psychrolimnae , Aszalós et al, 2020 and F. degerlachei , Zeglin et al, 2016 ) ( Table 3 and Supplementary Table 3 ). Pseudomonas extremaustralis (sq55) was also detected in Arnarnesstrýtan vent fluids and has previously been observed in other alkaline hot springs ( Table 3 ; Wang and Pecoraro, 2021 ). Furthermore, this ASV (Sq55_Pseduomonas) was not detected in background seawater samples.…”

Section: Resultssupporting

confidence: 70%

“…degerlachei, Zeglin et al, 2016) (Table 3 and Supplementary Table 3). Pseudomonas extremaustralis (sq55) was also detected in Arnarnesstrýtan vent fluids and has previously been observed in other alkaline hot springs (Table 3; Wang and Pecoraro, 2021). Furthermore, this ASV (Sq55_Pseduomonas) was not detected in background seawater samples.…”

Section: Microbial Community Of Arnarnesstrýtan Ventsupporting

confidence: 61%

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Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland

et al. 2022

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Strýtan Hydrothermal Field (SHF) is a submarine system located in Eyjafördur in northern Iceland composed of two main vents: Big Strýtan and Arnarnesstrýtan. The vents are shallow, ranging from 16 to 70 m water depth, and vent high pH (up to 10.2), moderate temperature (Tmax ∼70°C), anoxic, fresh fluids elevated in dissolved silica, with slightly elevated concentrations of hydrogen and methane. In contrast to other alkaline hydrothermal vents, SHF is unique because it is hosted in basalt and therefore the high pH is not created by serpentinization. While previous studies have assessed the geology and geochemistry of this site, the microbial diversity of SHF has not been explored in detail. Here we present a microbial diversity survey of the actively venting fluids and chimneys from Big Strýtan and Arnarnesstrýtan, using 16S rRNA gene amplicon sequencing. Community members from the vent fluids are mostly aerobic heterotrophic bacteria; however, within the chimneys oxic, low oxygen, and anoxic habitats could be distinguished, where taxa putatively capable of acetogenesis, sulfur-cycling, and hydrogen metabolism were observed. Very few archaea were observed in the samples. The inhabitants of SHF are more similar to terrestrial hot spring samples than other marine sites. It has been hypothesized that life on Earth (and elsewhere in the solar system) could have originated in an alkaline hydrothermal system, however all other studied alkaline submarine hydrothermal systems to date are fueled by serpentinization. SHF adds to our understandings of hydrothermal vents in relationship to microbial diversity, evolution, and possibly the origin of life.

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Section: Resultssupporting

confidence: 70%

Section: Microbial Community Of Arnarnesstrýtan Ventsupporting

confidence: 61%

Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland

et al. 2022

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“…Microscopy was done using a Nikon ECLIPSE Ci-L microscope (Tokyo, Japan) to examine fungal morphological characters, such as hyphae, pseudohyphae, conidiophores, conidia, poroconidia, arthroconidia, etc. ( Wang and Pecoraro, 2021 ). All isolated fungal strains were deposited in the LP Culture Collection (personal culture collection held in the laboratory of Prof. Lorenzo Pecoraro), at the School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.…”

Section: Methodsmentioning

confidence: 99%

Environmental Factors Shaping the Diversity and Spatial-Temporal Distribution of Indoor and Outdoor Culturable Airborne Fungal Communities in Tianjin University Campus, Tianjin, China

Yuan

Wang²,

Pecoraro³

2022

Front. Microbiol.

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Airborne fungi have significant influence on air quality and may pose adverse effects on human health due to their allergenic and pathogenic effect. We carried out a 1-year survey on the airborne fungal diversity and concentration of indoor and outdoor environments, within the Tianjin University campus. Airborne fungi were sampled using an HAS-100B air sampler. Isolated fungal strains were identified based on morphological and molecular analysis. A total of 641 fungal strains belonging to 173 species and 74 genera were identified in this study. The dominant fungal genera were Cladosporium (29.49%), Alternaria (25.9%), and Epicoccum (6.24%), while the most frequently occurring species were A. alternata (15.44%), C. cladosporioides (11.86%), and E. nigrum (5.77%). The mean fungal concentration at different sites ranged from 0 to 420 CFU/m3, which is lower than the permissive standard level. There was a seasonal variation in the airborne fungal community, while temperature and relative humidity were positively correlated with the fungal concentration and diversity at almost all the sites. Higher fungal diversity was recorded in peak period of human traffic at the two canteens, whereas the two dormitories showed higher fungal diversity in off-peak period. Our study provides the first report on the diversity and concentration of airborne fungal species within different Tianjin University campus environments and clarifies the role played by environmental factors on the analyzed fungal community. Our results may represent valuable information for air quality monitoring and microbial pollution control in densely populated human living environments.

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“…Fungal DNA sequences were submitted to GenBank under accessions ON711628-ON712642. Fungal morphology was characterized based on macroscopic and microscopic observations ( Pecoraro et al, 2020 ; Wang and Pecoraro, 2021 ). Microscopic morphological traits, such as septate hyphae, phialides, hyphal coils, conidiophores, conidia, and conidial chains were examined under a Nikon ECLIPSE Ci-L microscope (Tokyo, Japan) for identification of isolates following the taxonomic keys for different taxa ( Gilman, 1957 ; Lucas, 1981 ; De Hoog et al, 2000 ).…”

Section: Methodsmentioning

confidence: 99%

Seasonal variation of airborne fungal diversity and community structure in urban outdoor environments in Tianjin, China

Nageen

Wang²,

Pecoraro³

2023

Front. Microbiol.

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Airborne fungi are ubiquitous in human living environments and may be a source of respiratory problems, allergies, and other health issues. A 12 months study was performed to investigate the diversity, concentration and community structure of culturable airborne fungi in different outdoor environments of Tianjin City, using an HAS-100B air sampler. A total of 1,015 fungal strains belonging to 175 species and 82 genera of Ascomycota 92.5%, Basidiomycota 7%, and Mucoromycota 0.3% were isolated and identified using morphological and molecular analysis. The most abundant fungal genera were Alternaria 35%, Cladosporium 18%, Penicillium 5.6%, Talaromyces 3.9%, Didymella 3%, and Aspergillus 2.8%, while the most frequently occurring species were A. alternata (24.7%), C. cladosporioides (11%), A. tenuissima (5.3%), P. oxalicum (4.53%), and T. funiculosus (2.66%). The fungal concentration ranged from 0 to 340 CFU/m3 during the whole study. Environmental factors, including temperature, relative humidity, wind speed, and air pressure exerted a varying effect on the presence and concentration of different fungal taxa. The four analyzed seasons showed significantly different airborne fungal communities, which were more strongly influenced by air temperature and relative humidity in spring and summer, whereas wind speed and air pressure had a stronger effect in autumn and winter. Fungal communities from green and busy sites did not show significant differences over the four analyzed seasons, which may be due to the effect of the surrounding environments characterized by high human activities on the air of the relatively small parks present in Tianjin. The present study provided valuable information on the seasonal dynamics and the environmental factors shaping the diversity and concentration of the analyzed outdoor airborne fungal communities, which can be of help for air quality monitoring, microbial contamination control, and health risk assessment in urban environments.

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Diversity and Co-Occurrence Patterns of Fungal and Bacterial Communities from Alkaline Sediments and Water of Julong High-Altitude Hot Springs at Tianchi Volcano, Northeast China

Cited by 9 publications

References 71 publications

Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland

Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland

Environmental Factors Shaping the Diversity and Spatial-Temporal Distribution of Indoor and Outdoor Culturable Airborne Fungal Communities in Tianjin University Campus, Tianjin, China

Seasonal variation of airborne fungal diversity and community structure in urban outdoor environments in Tianjin, China

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