Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area

Abstract

This study assessed the diversity and composition of bacterial communities within soils and lake sediments from an Arctic lake area (London Island, Svalbard). A total of 2,987 operational taxonomic units were identified by high-throughput sequencing, targeting bacterial 16S rRNA gene. The samples from four sites (three samples in each site) were significantly different in geochemical properties and bacterial community composition. Proteobacteria and Acidobacteria were abundant phyla in the nine soil samples, whereas Proteobacteria and Bacteroidetes were abundant phyla in the three sediment samples. Furthermore, Actinobacteria, Chlorobi, Chloroflexi, Elusimicrobia, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria significantly varied in their abundance among the four sampling sites. Additionally, members of the dominant genera, such as Clostridium, Luteolibacter, Methylibium, Rhodococcus, and Rhodoplanes, were significantly different in their abundance among the four sampling sites. Besides, distance-based redundancy analysis revealed that pH (p < 0.001), water content (p < 0.01), ammonium nitrogen (NH4+-N, p < 0.01), silicate silicon (SiO42--Si, p < 0.01), nitrite nitrogen (NO2--N, p < 0.05), organic carbon (p < 0.05), and organic nitrogen (p < 0.05) were the most significant factors that correlated with the bacterial community composition. The results suggest soils and sediments from a lake area in the Arctic harbor a high diversity of bacterial communities, which are influenced by many geochemical factors of Arctic environments.

Microbial genomes retrieved from High Arctic lake sediments encode for adaptation to cold and oligotrophic environments

“…However, several groups that were found here to be 320 highly prevalent (Archaea, Omnitrophica) were likely missed in the earlier study because of PCR 321 selection bias (Suzuki and Giovannoni, 1996). The community was also similar to previous 322 metagenomic studies of oligotrophic lake sediments (e.g., Wang et al, 2016). One notable exception to 323 previous studies (e.g., Rautio et al, 2011) was that Cyanobacteria were much less common in Lake 324…”

“…To date, culture-independent studies of microbial communities in Arctic lakes have been most 50 often performed by amplifying and sequencing taxonomic markers like the 16S rRNA gene (e.g., 51 Crump et al, 2012;Mohit et al, 2017;Ruuskanen et al, 2018;Stoeva et al, 2014;Wang et al, 2016Wang et al, , 52 2019). However, amplicon-based methods are subject to PCR amplification bias, which might alter the 53 estimates of microbial community composition and diversity.…”

Increases in temperature and nutrient availability positively affect methane‐cycling microorganisms in Arctic thermokarst lake sediments

“…The sediment cores had a relatively rare bacterial biosphere where 50% of the reads was affiliated to groups with an individual abundance of less than 2%. This high bacterial diversity was not observed in previously described thaw ponds/freshwater sediments (Liebner et al ., ; Rossi et al ., ; Crevecoeur et al ., ; Wang et al ., ; Wagner et al ., ). The dominant members of the bacterial community were Bacteriodetes (16%–19%) similar to a study of polygonal tundra in the Lena Delta, Siberia, where Bacteroidetes constituted almost 50% of the soil microbial community (Liebner et al ., ).…”

“…The dominant members of the bacterial community were Bacteriodetes (16%–19%) similar to a study of polygonal tundra in the Lena Delta, Siberia, where Bacteroidetes constituted almost 50% of the soil microbial community (Liebner et al ., ). Most studies of permafrost thaw ponds that used different DNA extraction and amplification methods, however, have found Proteobacteria to be the most dominant Bacteria (Rossi et al ., ; Crevecoeur et al ., ; Wang et al ., ; Wagner et al ., ), a pattern that was also shown by Wagner et al . () who used the same extraction method and 16S rRNA gene amplification as our study.…”

Short‐term Effects of Great Cormorant Droppings on Water Quality and Microbial Community of an Artificial Agricultural Reservoir

“…It was apparent that geochemical gradients within sediment and the water column yielded different contributions with regard to microbial diversity. Therefore, distinct patterns of microbial diversity across sediment and the water column were likely to be caused by differences in redox states and nutrient availability within the lake (Jiang et al, 2006; Wang et al, 2016). The NMDS generally showed a higher dispersion of plots belonging to sediment microcosms with a greater mass of added feces (Fig.…”