Diversity and distribution of 16S rRNA and phenol monooxygenase genes in the rhizosphere and endophytic bacteria isolated from PAH-contaminated sites

Peng, Anping; Liu, Juan; Ling, Wanting; Chen, Zeyou; Gao, Yanzheng

doi:10.1038/srep12173

Cited by 21 publications

(6 citation statements)

References 51 publications

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“…Taking into consideration the limitations of DGGE fingerprinting of the 16S rDNA (Muyzer et al ), the technique is still of practical use to study microbial community structure and diversity (Hu et al , Peng et al ). Therefore we used culture‐dependent DGGE fingerprinting of the 16S rDNA (Edenborn and Sexstone ) to characterize the bacterial communities, CFUs, developed on agar plates representing all of the tested culture media.…”

Section: Discussionmentioning

confidence: 99%

Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria

Sarhan

Mourad

Hamza

et al. 2016

Physiologia Plantarum

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We have developed teabags packed with dehydrated plant powders, without any supplements, for preparation of plant infusions necessary to develop media for culturing rhizobacteria. These bacteria are efficiently cultivated on such plant teabag culture media, with better progressive in situ recoverability compared to standard chemically synthetic culture media. Combining various plant-based culture media and incubation conditions enabled us to resolve unique denaturing gradient gel electrophoresis (DGGE) bands that were not resolved by tested standard culture media. Based on polymerase chain reaction PCR-DGGE of 16S rDNA fingerprints and sequencing, the plant teabag culture media supported higher diversity and significant increases in the richness of endo-rhizobacteria, namely Gammaproteobacteria (Enterobacteriaceae) and predominantly Alphaproteobacteria (Rhizobiaceae). This culminated in greater retrieval of the rhizobacteria taxa associated with the plant roots. We conclude that the plant teabag culture medium by itself, without any nutritional supplements, is sufficient and efficient for recovering and mirroring the complex and diverse communities of rhizobacteria. Our message to fellow microbial ecologists is: simply dehydrate your plant canopy, teabag it and soak it to prepare your culture media, with no need for any additional supplementary nutrients.

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Section: Discussionmentioning

confidence: 99%

Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria

Sarhan

Mourad

Hamza

et al. 2016

Physiologia Plantarum

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“…Therefore, it is assumed that the initial pollution level of PAHs played an important role in the dissipation quantity of PAHs. In fact, PAH contamination level may play a decisive role in bacterial community structure by affecting the distribution, diversity, and levels of rhizosphere bacteria (Peng et al 2015), and this might also substantially affect the removal efficiency of PAHs. Other factors influencing the removal efficiency of PAHs might be the microbial community structure, soil environmental condition, plant species and the profile of PAHs.…”

Section: Resultsmentioning

confidence: 99%

Rhizosphere effect on removal and bioavailability of PAHs in contaminated agricultural soil

Możdżeń

Zhang

et al. 2021

Biologia

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The rhizosphere effect of ryegrass (Lolium perenne L.) on polycyclic aromatic hydrocarbons (PAHs) dissipation, bioavailability and the structure change of microbial community was investigated using a compartmented device-rhizobox. The PAHs removal efficiency, bioavailability and the change in structure of the microbial community were ascertained using HPLC, Tenax-TA extraction and PCR-DGGE, respectively. The results showed that in the root area (R1) and bulk soil (CK), the removal of 3-ring PAHs were 97.72 ± 0.34% and 95.51 ± 0.75%, 4-ring PAHs were 89.01 ± 1.61% and 78.65 ± 0.47%, 5-ring PAHs were 77.64 ± 4.05% and 48.63 ± 3.19%, 6-ring PAHs were 68.69 ± 3.68% and 36.09 ± 1.78%, respectively. The average removal efficiency of the total PAHs after 80 days followed the order: R1M (91.1%) > CKM (84.9%) > CK (77.6%), indicating that planted soil with inoculation of Mycobacterium sp. as well as non-planted soil inoculated with Mycobacterium sp. could both significantly accelerate the removal of PAHs compared to control soil. The bioavailability ratio of PAHs with 3 and 4 rings tended to decrease (from 59.9% to 14.8% for 3-ring and 7.61% to 5.08% for 4-ring, respectively in R1) while those with 5 rings increased significantly (from 2.41% to 33.78% in R1) during the last 40 days, indicating that bioavailability alteration varies with the number of rings in the PAHs. In addition, PAH bioavailability generally did not show a significant difference between treated soil and control soil. These results suggest that ryegrass rhizosphere effect as well as inoculation of Mycobacterium sp. can accelerate PAH removal in polluted soil. The bacteria community structure demonstrated a complex interplay of soil, bacteria and ryegrass root, and potential PAH degraders were present in abundance. This study provides the exploring data of rhizosphere and bioaugmentation effect on PAH dissipation in agricultural soil, as well as the change of bioavailability and microbial composition thereof.

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“…Ambientes contaminados com fenol estão relacionados a uma maior expressão de genes da classe das monooxigenases, que desempenham papel importante na hidrolização catalítica de compostos aromáticos. Esses genes estão presentes nos micro-organismos rizosfericos e endofíticos e há evidências de transferência horizontal de genes de degradação entre esses grupos (WANG et al, 2007;PENG et al, 2015). Devido a versatilidade metabólica, os endofíticos podem ser uma boa alternativa para busca de biorremediadores, possuindo simultaneamente a capacidade de degradar tanto o fenol como os hidrocarbonetos, como no caso dos isolados 6F, 7F, 8F e 10F.…”

Section: Resultsunclassified

Avaliação Da Capacidade De Degradação De Hidrocarbonetos E Fenol Por Isolados Bacterianos Endofíticos De Bambu

Ribeiro¹,

Oliveira²,

Rodrigues³

et al. 2016

Encicl. Biosf.

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Endophytic bacteria exhibit a wide biotechnological potential, not limited to the plant growth promotion, and often also described by their bioremediation capacity. These microorganisms participate from the hydrocarbon degradation and other organic pollutants, to heavy metals remediation, elevating significantly the efficiency of phitoremediation systems. The aim of this study was to evaluate qualitatively the hydrocarbon degradation capacity and phenol tolerance by endophytic bacteria from bamboo. For the hydrocarbon degradation screening, it was performed tests in microplates with the microbial cultivation using each one of the following compounds as a sole carbon source: diesel oil, gasoline, kerosene, burned oil, lubricants (20W40, 20W50, 5W50 and 15W40). For phenol tolerance analysis, the endophytic were cultivated in mineral minimum solid medium, using phenol as the sole carbon source in three concentrations: 50, 100 and 150 mg/L. For the 21 evaluated bacterial isolates, one isolate (7F) presented the fuel degradation capacity (diesel oil, gasoline and kerosene), and two isolates (9F and 6F) exhibited partial degradation capacity. The vegetal oil degradation was observed for three isolates (6F, 7F and 9F) and partially for the isolate 10F. The degradation for the tested lubricants was observed partially for one isolate (7F), with the partial degradation for 5W50 and 15W40 oils by the 8F isolate. Fifteen isolates were tolerant to phenol in all the analyzed concentrations. The results suggest a potential application of the endophytic bacterial isolates in bioremediation process.

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Diversity and distribution of 16S rRNA and phenol monooxygenase genes in the rhizosphere and endophytic bacteria isolated from PAH-contaminated sites

Cited by 21 publications

References 51 publications

Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria

Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria

Rhizosphere effect on removal and bioavailability of PAHs in contaminated agricultural soil

Avaliação Da Capacidade De Degradação De Hidrocarbonetos E Fenol Por Isolados Bacterianos Endofíticos De Bambu

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