Diversity and distribution of cyanobacterial toxins in the Italian subalpine lacustrine district

Cerasino, Leonardo; Salmaso, Nico

doi:10.2478/s13545-012-0028-9

Cited by 46 publications

(25 citation statements)

References 44 publications

(30 reference statements)

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“…The most abundant congeners were LR in M. aeruginosa and RRdm in P. rubescens. The relative abundances and identities of congeners were in accordance with previous observations (Cerasino and Salmaso, 2012;Salmaso et al, 2014;Cerasino et al, 2016) Fig. 3.…”

Section: Discussionsupporting

confidence: 91%

A comparative study of the metabolic profiles of common nuisance cyanobacteria in southern perialpine lakes

Cerasino¹,

Capelli²,

Salmaso³

2017

Adv Ocean Limnol

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This work allowed the comparison of the metabolic profiles of the most important cyanobacteria species in southern perialpine lakes, namely Aphanizomenon flos-aquae, Dolichospermum lemmermannii, Microcystis aeruginosa, Planktothrix rubescens, and Tychonema bourrellyi. Monospecific cultures were obtained from samples of 3 different natural lakes (Garda, Idro, and Caldonazzo). LC-MS/MS analyses were conducted on strains. A first set of experiments was aimed at assessing the presence of the best known toxins (microcystins, nodularins, (homo)anatoxin-a, cylindrospermopsins, paralytic shellfish poisons) in the cultures. Results of this screening study revealed that M. aeruginosa and P. rubescens produced toxic peptides (microcystins), T. bourrellyi produced toxic alkaloids (anatoxin-a and possibly some paralytic shellfish toxins), Aph. flos-aquae and D. lemmermannii did not produce any of the analyzed toxins. M. aeruginosa and P. rubescens showed typical microcystin production with LR form dominant in the former, and RRdm form dominant in the latter. A second set of experiments was aimed at comparing the capability of the 5 cyanobacterial species to produce peptidic secondary metabolites. For this purpose, an untargeted peptidomic analysis was conducted on the strains. The analysis allowed revealing globally 328 metabolites, spanning in a mass range between 400 and 2000 Da. The majority of compounds with masses in the 500-1200 Da range (corresponding to the majority of peptidic secondary metabolites) resulted to be produced by M. aeruginosa and P. rubescens strains, thus indicating a higher ability of these species to produce non-ribosomal peptides compared to the others. 27 metabolites out of 328 could be putatively assigned to specific classes of compounds: microcystins, aeruginosins and anabaenopeptins were the most represented classes of compounds, and were mostly found in M. aeruginosa and P. rubescens strains.

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Section: Discussionsupporting

confidence: 91%

A comparative study of the metabolic profiles of common nuisance cyanobacteria in southern perialpine lakes

Cerasino¹,

Capelli²,

Salmaso³

2017

Adv Ocean Limnol

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“…In Italy, reports of ATX are quite rare, refer ring exclusively to environmental samples (e.g., Cerasino and Salmaso, 2012). The production of ATX was associated with blooms or higher development of Anabaena planctonica (Doli chospermum planctonicum) (Bruno et al, 1994), Anabaena crassa (D. crassum) (Messineo et al, 2009) and "a peculiar P. rubescens population" (Viaggiu et al, 2004;Messineo et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In a recent work, Cerasino and Salmaso (2012) documented a widespread presence of ATX in the lake district south of the Alps. Based on analyses carried out on environmental sam pies collected during the warmer months, detectable con centrations of ATX ranging between 0.1 and 0.6 ~~g L 1 were found in the lakes Garda, Iseo, Como and Maggiore, i.e.…”

Section: Introductionmentioning

confidence: 99%

Anatoxin-a producing Tychonema (Cyanobacteria) in European waterbodies

et al. 2015

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Keywords:Anatoxin a Tychonema Cell quotaPolyphasic approach Phylogenetic analysis European waterbodies a b s t r a c tIn order to identify the cyanobacterial species responsible of anatoxin a (ATX) production in Lake Garda (Northern Italy), an intensive isolation and culturing of filamentous cyano bacteria were established since 2014 from environmental samples. In this work, we report a detailed account of the strategy adopted, which led to the discovery of a new unexpected producer of ATX, Tychonema bourrellyi. So far, this species is the first documented example of cultured Oscillatoriales able to produce ATX isolated from pelagic freshwater ecosys tems. The isolated filaments were identified adopting a polyphasic approach, which included microscopic species identification, genetic characterisation and phylogenetic analyses based on 16S rRNA genes. The taxonomic identification was further confirmed by the high (>99%) rbcLX sequence similarities of the T. bourrellyi strains of Lake Garda with those deposited in DNA sequence databases. More than half of the isolates were shown to produce a significant amount of ATX, with cell quota ranging between 0.1 and 2.6 mg mm 3 , and 0.01 and 0.35 pg cell 1 . The toxic isolates were tested positive for anaC of the anatoxin a synthetase (ana) gene cluster. These findings were confirmed with the discovery of one ATX producing T. bourrellyi strain isolated in Norway. This strain and a further non ATXproducing Norwegian Tychonema bornetii strain tested positive for the presence of the anaF gene of the ana gene cluster. Conversely, none of the Italian and Norwegian Tychonema strains were positive for microcystins (MCs), which was also confirmed by the absence of mcyE PCR products in all the samples analysed. This work suggests that the only reliable strategy to identify cyanotoxins producers should be based on the isolation of strains and their identification with a polyphasic approach associated to a concurrent metabolomic profiling.

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“…MCs were determined by LC-MS/MS (Waters Acquity UPLC 1 directly coupled to an AB Sciex 4000 QTRAP hybrid triple quadrupole-linear ion trap mass spectrometer). The analyses followed the methods described in Guzzella et al (2010) and Cerasino and Salmaso (2012).…”

Section: Sampling and Analytical Methodsmentioning

confidence: 99%

Strict coupling between the development ofPlanktothrix rubescensand microcystin content in two nearby lakes south of the Alps (lakes Garda and Ledro)

Salmaso

Adriano

Shams

et al. 2013

Ann. Limnol. - Int. J. Lim.

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-Cyanobacteria and their principal cyanotoxins were studied in the largest Italian lake (Lake Garda, 65 m a.s.l.) and in a near smaller reservoir (Lake Ledro, 652 m a.s.l.). The two lakes share a fraction of water due to a pipe pumping water from and into the lakes and the same dominant cyanobacterial species (Planktothrix rubescens). Despite the differences in the concentrations of cyanotoxins (mostly microcystins, MCs) and biovolumes of P. rubescens (BV Pr ) (over one order of magnitude), the Bayesian analyses linking these two variables showed striking similarities, suggesting the existence of similar toxic genotypes colonizing the two water bodies and a constitutive MC production. It was stressed that a greater sensitivity and reliability in the management strategies aimed at minimizing the risks due to cyanobacteria should also contemplate the use of specific lake-tailored models linking MCs and BV Pr .

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Diversity and distribution of cyanobacterial toxins in the Italian subalpine lacustrine district

Cited by 46 publications

References 44 publications

A comparative study of the metabolic profiles of common nuisance cyanobacteria in southern perialpine lakes

A comparative study of the metabolic profiles of common nuisance cyanobacteria in southern perialpine lakes

Anatoxin-a producing Tychonema (Cyanobacteria) in European waterbodies

Strict coupling between the development ofPlanktothrix rubescensand microcystin content in two nearby lakes south of the Alps (lakes Garda and Ledro)

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