Diversity and distribution of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) and their bacterial symbionts (γ-Proteobacteria: Enterobacteriaceae) in Jordan

Stock, S. Patricia; Banna, Luma Al; Darwish, Rula M.; Katbeh, Ahmad

doi:10.1016/j.jip.2008.01.003

Cited by 21 publications

(6 citation statements)

References 23 publications

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“…The natural occurrence of EPNs has been documented in different soils, ranging from natural and managed ecosystems of all continents with the exception of Antarctica (Laznik and Trdan 2011). Numerous surveys in agricultural (managed) areas were conducted to collect and describe native EPNs using morphological and molecular diagnostic tools for the management of local target pests (Stock et al 2008). These native EPN populations have mainly gained an increasing attention due to its beneficial attributes like better adaptation to local biotic and abiotic conditions (Stock et al 2008 andCampos-Herrera et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Numerous surveys in agricultural (managed) areas were conducted to collect and describe native EPNs using morphological and molecular diagnostic tools for the management of local target pests (Stock et al 2008). These native EPN populations have mainly gained an increasing attention due to its beneficial attributes like better adaptation to local biotic and abiotic conditions (Stock et al 2008 andCampos-Herrera et al 2012). At present, about 100 Steinernema and 16 Hetererhabditis have been described worldwide (Bhat et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Survey and identification of entomopathogenic nematodes in the province of Cotabato, Philippines, for biocontrol potential against the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

Dichusa

Ramos

Aryal

et al. 2021

Egypt J Biol Pest Control

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Background The tobacco cutworm, Spodoptera litura [Fab.] (Lepidoptera: Noctuidae), is a devastating insect pest of several crops. Entomopathogenic nematodes (EPNs) of the families Heterorhabditidae and Steinernematidae are used as an alternative control measure in lieu of the hazardous synthetic chemical applications. Results A survey of naturally occurring EPNs was conducted across the province of Cotabato, Philippines, covering a total of 5 municipalities with 25 villages. Galleria-baiting technique was employed to recover nematodes from peanut and grassland soils. Out of 50 soil samples collected, only 5 samples harbored nematodes, indicating a recovery of 10%. Preliminary morphological data identified only one EPN under the genera Heterorhabditis (1 isolate), whereas 4 were facultative necromenic nematodes from the genera Metarhabditis (2 isolates) and Oscheius (2 isolates). Analysis of D2D3 segments of the 28S rDNA confirmed high sequence similarity to Heterorhabditis indica, Metarhabditis rainai, Oscheius insectivora, and Oscheius sp. This is the first record of H. indica and M. rainai in the entire region, whereas the first record for Oscheius spp. in the Philippines. Furthermore, the biocontrol potential of the local H. indica infective juvenile (IJ) populations (PIGCD1) isolated from peanut was assessed against the tobacco cutworm, S. litura, under laboratory conditions. The mean percentage mortality caused by H. indica on S. litura at 7 different concentrations ranged from 0-100% at 24 h post inoculation. The lethal concentration (LC50) required to kill 50% of the S. litura larvae population with H. indica was 7.13±1 (IJs/larva). Conclusions The use of Galleria-baiting method is a convenient approach to detect EPNs including other facultative necromenic nematodes from the soils. Obtained data indicated that the local H. indica isolate can be a promising alternative measure to suppress the economically important insect pest, S. litura, and this may provide significant outlook to establish the biocontrol program in the country.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Survey and identification of entomopathogenic nematodes in the province of Cotabato, Philippines, for biocontrol potential against the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

Dichusa

Ramos

Aryal

et al. 2021

Egypt J Biol Pest Control

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“…The prevalence of EPN in the soil samples in our research is low (3.28%). This result is not unusual and aligns with results from neighboring countries, such as 2% in Türkiye ( Hazir et al ., 2003 ), 0.9% in Jordan ( Stock et al ., 2008 ), 1% in Lebanon ( Noujeim et al ., 2011 ), and 3.2% in northwestern Iran ( Eivazian Kary et al ., 2009 ). Globally, the prevalence of EPNs varied widely, ranging from 0.7% to 100% ( Bruck, 2004 ; Campos-Herrera et al ., 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification of entomopathogenic nematodes with insights into their distribution in the Syrian coast regions and virulence against Tuta absoluta

Ali,

Allouf,

Ahmad

2023

Journal of Nematology

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The occurrence and distribution of entomopathogenic nematodes (EPNs) in the Syrian coast regions remain relatively uncharted. To address this gap in our knowledge, an extensive survey of these ecosystems was essential. This study aims to isolate and identify EPNs from diverse ecosystems within the coastal regions. The distribution of EPNs in cultivated and natural environments was analyzed according to habitat, altitude, and sampling season factors. Between 2017 and 2020, EPNs were recovered from 27 out of 821 soil samples (3.28%) and collected from 24 out of 375 sampling sites (6.4%). Based on morphological, morphometric, and molecular (ITS) characteristics, four EPN species were identified: Heterorhabditis indica (51.85%), representing the first report of its occurrence in the coastal regions, H. bacteriophora (33.33%), H. pakistanense (7.4%), which is also reported for the first time in Syria, and Steinernema affine (7.4%). There were statistical differences in the abundance and recovery frequency of EPNs in each type of habitat. Additionally, there were statistical differences in the altitude and sampling season recovery frequency. Co-inertia analysis revealed correlation between the distribution and occurrence of EPNs in vegetation habitats, altitude, and sampling seasons, as well as some soil characteristics. H. indica and H. bacteriophora were associated with citrus orchards, low-altitude ranges, moderate organic matter, and acidic soil. More specifically, H. indica isolates were correlated with olive orchards, vegetable fields, autumn season, and clay, sandy, and sandy loam soils. Meanwhile, H. bacteriophora isolates were correlated with tobacco fields, grasslands, alkaline pH, spring season, silty loam, and clay loam soils. H. pakistanense was linked to pear orchards, vineyards, moderate pH, and low organic matter. S. affine occurred in walnut orchards, silty soil, higher altitudes, and winter season. The virulence levels of three native EPN isolates (S. affine, H. indica and H. bacteriophora) were evaluated against 3rd and 4th instar larvae (outside and inside mines) and pupae of T. absoluta, a destructive pest in Syria. All three native EPN species exhibited ability to infect and kill the insect, with observed significant differences in their virulence. This study provides an understanding of EPN occurrence, distribution, and their potential for application in sustainable pest control strategies in Syria.

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“…The relationship between habitat features and abundance of EPNs has been stated in several studies. It has been reported that there are high occurrence rates of EPNs in disturbed habitats, such as agricultural areas with high anthropogenic influences [38][39][40]. However, in undisturbed habitats, abundance of EPNs is relatively higher [5,[41][42][43].…”

Section: Discussionmentioning

confidence: 99%

First Record of the Entomopathogenic Nematode Steinernema litorale (Filipjev) (Rhabditida: Steinernematidae) and Its Symbiotic Bacterium from Turkey, and Its Efficacy Capability

Özdemir

Bayram

Susurluk

2020

Insects

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The entomopathogenic nematode Steinernema litorale was isolated from Çamkoru Nature Park located in Ankara, Turkey, in September 2018. Steinernema litorale was recovered in 1 of 67 soil samples from a natural forest area; the soil was characterised as sandy loam. The isolated nematode S. litorale was identified based on morphological and molecular parameters. The symbiotic bacterium of S. litorale was determined as Xenorhabdus bovienii. Steinernema litorale was found for the first time in Turkey and the Middle East. The virulence of the isolate was tested on Galleria mellonella larvae. Different concentrations of the nematode (10, 25, 50, 75, and 100 infective juveniles (IJs/larvae) were used. While the LC50 values at 48 h, 72 h, and 96 h were 153.419, 51.005, and 15.439 IJs, respectively, and the LT50 values at 75 IJs and 100 IJs showed that this isolate is capable to control insect larvae within 50.083 and 36.266 h, respectively.

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Diversity and distribution of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) and their bacterial symbionts (γ-Proteobacteria: Enterobacteriaceae) in Jordan

Cited by 21 publications

References 23 publications

Survey and identification of entomopathogenic nematodes in the province of Cotabato, Philippines, for biocontrol potential against the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

Survey and identification of entomopathogenic nematodes in the province of Cotabato, Philippines, for biocontrol potential against the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

Isolation, identification of entomopathogenic nematodes with insights into their distribution in the Syrian coast regions and virulence against Tuta absoluta

First Record of the Entomopathogenic Nematode Steinernema litorale (Filipjev) (Rhabditida: Steinernematidae) and Its Symbiotic Bacterium from Turkey, and Its Efficacy Capability

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