Diversity and Dynamics of Methanotrophs within an Experimental Landfill Cover Soil

Jugnia, Louis‐B.; Aït-Benichou, Samah; Fortin, Nathalie; Cabral, Alexandre R.; Greer, Charles W.

doi:10.2136/sssaj2008.0161

Cited by 11 publications

(5 citation statements)

References 54 publications

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“…DNA was extracted from 10 g of the native soil samples and from one of the triplicate microcosms for each incubation treatment following the protocol of Fortin et al (18). DNA crude extracts from the native soil samples were purified using polyvinylpolypyrrolidone (PVPP)/Sephacryl spin columns (26) and used for PCR-DGGE (PCR and denaturing gradient gel electrophoresis [DGGE]) and quantitative PCR (qPCR) assays. DNA crude extracts from the samples incubated with 13 CH 4 were loaded onto cesium chloride (CsCl) density gradients together with SYBR safe (Invitrogen, Carlsbad, CA) as described by Martineau et al (34).…”

Section: Methodsmentioning

confidence: 99%

“…4B). With the exception of bands 16,20,26,27, and 31, which exhibited 93 to 95% identity with Methylosarcina lacus (GenBank accession no. AY007286), all the pmoA amino acid sequences retrieved from the heavy DNA fractions of soil samples from Eureka exhibited more than 94% identity to members of the genus Methylobacter (Fig.…”

Section: Soil Characteristics the Mean Annual Temperatures At Eumentioning

confidence: 99%

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Stable Isotope Probing Analysis of the Diversity and Activity of Methanotrophic Bacteria in Soils from the Canadian High Arctic

Martineau

Whyte

Greer

2010

Appl Environ Microbiol

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108

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The melting of permafrost and its potential impact on CH 4 emissions are major concerns in the context of global warming. Methanotrophic bacteria have the capacity to mitigate CH 4 emissions from melting permafrost. Here, we used quantitative PCR (qPCR), stable isotope probing (SIP) of DNA, denaturing gradient gel electrophoresis (DGGE) fingerprinting, and sequencing of the 16S rRNA and pmoA genes to study the activity and diversity of methanotrophic bacteria in active-layer soils from Ellesmere Island in the Canadian high Arctic. Results showed that most of the soils had the capacity to oxidize CH 4 at 4°C and at room temperature (RT), but the oxidation rates were greater at RT than at 4°C and were significantly enhanced by nutrient amendment. The DGGE banding patterns associated with active methanotrophic bacterial populations were also different depending on the temperature of incubation and the addition of nutrients. Sequencing of the 16S rRNA and pmoA genes indicated a low diversity of the active methanotrophic bacteria, with all methanotroph 16S rRNA and pmoA gene sequences being related to type I methanotrophs from Methylobacter and Methylosarcina. The dominance of type I methanotrophs over type II methanotrophs in the native soil samples was confirmed by qPCR of the 16S rRNA gene with primers specific for these two groups of bacteria. The 16S rRNA and pmoA gene sequences related to those of Methylobacter tundripaludum were found in all soils, regardless of the incubation conditions, and they might therefore play a role in CH 4 degradation in situ. This work is providing new information supporting the potential importance of Methylobacter spp. in Arctic soils found in previous studies and contributes to the limited body of knowledge on methanotrophic activity and diversity in this extreme environment.

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Section: Methodsmentioning

confidence: 99%

Section: Soil Characteristics the Mean Annual Temperatures At Eumentioning

confidence: 99%

Stable Isotope Probing Analysis of the Diversity and Activity of Methanotrophic Bacteria in Soils from the Canadian High Arctic

Martineau

Whyte

Greer

2010

Appl Environ Microbiol

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108

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“…Proteins and debris were precipitated using 7.5 M ammonium acetate, DNA was then precipitated overnight at −20 • C in cold 2-propanol, and resuspended in TE (pH 8.0). DNA crude extracts were purified using PVPP/Sephacryl spin columns as described in Jugnia et al (2009). Before the addition of the DNA extracts, resins were washed twice with TE (pH 7.5)-NaCl (0.1 M), to improve DNA recovery.…”

Section: Dna Extractionmentioning

confidence: 99%

Field performance of alder-Frankia symbionts for the reclamation of oil sands sites

Lefrançois

Quoreshi

Khasa

et al. 2010

Applied Soil Ecology

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“…Methylobacter, Methylocaldum and Methylomonas gene) and Type II (e.g. Methylosinus and Methylocystis gene) are responsible for CH4 oxidation and can be identified in biocovers (Gebert, 2009;Jugnia et al, 2009). Several materials can be used for the PMOB substrate, for example organic materials such as compost, sewage sludge, peat, soil or a mixture of them (Hilger and Humer, 2003;Humer and Lechner, 2001;Iranpour et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evaluating Methane Oxidation Efficiencies in Experimental Landfill Biocovers by Mass Balance and Carbon Stable Isotopes

Capanema

Cabral

2012

Water Air Soil Pollut

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Biocovers are an alternative for mitigating fugitive and residual emissions of methane from landfills.In this study, we evaluated the performance of two experimental passive methane oxidation biocovers (PMOBs) constructed within the existing final cover of the St-Nicéphore landfill (Quebec, Canada). The biocovers were fed in a controlled manner with raw biogas and surface fluxes were obtained using static chambers. This enabled calculating mass balances of CH4 and oxidation efficiencies (fo_MB). Most of the time, fo_MB ≥ 92% were obtained for loadings as high as 818 g CH4 m -2 d -1 (PMOB-2) and 290 g CH4 m -2 d -1 (PMOB-3B). The lowest efficiencies (fo_MB = 45.5% and 34.0%, respectively) were obtained during cold days (air temperature ~ 0°C). Efficiencies were also calculated using stable isotopes (fo_SI); the highest fo_SI were 66.4% for PMOB-2 and 87.3% for PMOB-3B; whereas the lowest were 18.8% and 23.1%, respectively. However, fo_SI values reflect CH4 oxidation up to a depth of 0.10 m, which may partly explain the difference in regards to mass balance-derived efficiencies. Indeed, it is expected that a significant fraction of the total CH4 oxidation occurs within the zone near the surface, where there is greater O2 availability. The influence of the values of fractionation factors ox and trans were also evaluated in this paper.

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Diversity and Dynamics of Methanotrophs within an Experimental Landfill Cover Soil

Abstract: Diversity and dynamics of methanotrophs within an experimental landfill cover soil Jugnia, Louis-B.; Aït-Benichou, Samah; Fortin, Nathalie; Cabral, Alexandre R.; Greer, Charles W.Contact us / Contactez nous: nparc.cisti@nrc-cnrc.gc.ca.

Cited by 11 publications

References 54 publications

Stable Isotope Probing Analysis of the Diversity and Activity of Methanotrophic Bacteria in Soils from the Canadian High Arctic

Stable Isotope Probing Analysis of the Diversity and Activity of Methanotrophic Bacteria in Soils from the Canadian High Arctic

Field performance of alder-Frankia symbionts for the reclamation of oil sands sites

Evaluating Methane Oxidation Efficiencies in Experimental Landfill Biocovers by Mass Balance and Carbon Stable Isotopes

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