Diversity and Dynamics of Salmonella enterica in Water Sources, Poultry Litters, and Field Soils Amended With Poultry Litter in a Major Agricultural Area of Virginia

Gu, Ganyu; Strawn, Laura K.; Zheng, Jie; Reed, Elizabeth; Rideout, Steven L.

doi:10.3389/fmicb.2019.02868

Cited by 44 publications

(66 citation statements)

References 33 publications

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“…In our previous study, the prevalence and population density of S. enterica in irrigation tested ponds and wells were reported ( Gu et al, 2019a ).…”

Section: Resultsmentioning

confidence: 99%

“…In this study, all the S. enterica isolates collected from irrigation ponds in the previous study ( Gu et al, 2019a ) were further subjected to antimicrobial susceptibility test. Among the 270 S. enterica isolates, 12 isolates were identified to be resistant to at least one of the tested antibiotics, including four Thompson isolates resistant to ceftiofur or tetracycline, three Typhimurium isolates resistant to streptomycin, tetracycline, or amoxicillin/clavulanic acid 2:1 ratio, two Saintpaul isolates resistant to ceftriaxone or ciprofloxacin, two Newport isolates resistant to ceftriaxone or cefoxitin and amoxicillin/clavulanic acid 2:1 ratio, and one Larochelle isolate resistant to cefoxitin.…”

Section: Resultsmentioning

confidence: 99%

“…In previous study, the prevalence and dynamics of S. enterica in irrigation ponds and wells on the ESV was reported. Spatial (farm location) and temporal (weekly) differences for S. enterica occurrence in surface pond water was detected ( Gu et al, 2019a ). In this study, the prevalence and dynamics of L. monocytogenes in irrigation ponds and wells on the ESV was investigated.…”

Section: Discussionmentioning

confidence: 99%

“…From January to December 2015, 4 L of pond and well irrigation water samples were collected weekly from four vegetable farms (Farms A–D) on ESV for S. enterica spp. detection ( Gu et al, 2019a ). At one of the sampling times per month for S. enterica detection, 4-L water samples were also collected concurrently from the same irrigation ponds and wells for L. monocytogenes detection.…”

Section: Methodsmentioning

confidence: 99%

“…A most probable number (MPN) method was used to assess S. enterica concentration in collected water samples as described previously ( Luo et al, 2014 ; Gu et al, 2019a , b ).…”

Section: Methodsmentioning

confidence: 99%

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Correlation of Salmonella enterica and Listeria monocytogenes in Irrigation Water to Environmental Factors, Fecal Indicators, and Bacterial Communities

Strawn

Ottesen

et al. 2021

Front. Microbiol.

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Outbreaks of foodborne illnesses linked to fresh fruits and vegetables have been key drivers behind a wide breadth of research aiming to fill data gaps in our understanding of the total ecology of agricultural water sources such as ponds and wells and the relationship of this ecology to foodborne pathogens such as Salmonella enterica and Listeria monocytogenes. Both S. enterica and L. monocytogenes can persist in irrigation water and have been linked to produce contamination events. Data describing the abundance of these organisms in specific agricultural water sources are valuable to guide water treatment measures. Here, we profiled the culture independent water microbiota of four farm ponds and wells correlated with microbiological recovery of S. enterica (prevalence: pond, 19.4%; well, 3.3%), L. monocytogenes (pond, 27.1%; well, 4.2%) and fecal indicator testing. Correlation between abiotic factors, including water parameters (temperature, pH, conductivity, dissolved oxygen percentage, oxidation reduction potential, and turbidity) and weather (temperature and rainfall), and foodborne pathogens were also evaluated. Although abiotic factors did not correlate with recovery of S. enterica or L. monocytogenes (p > 0.05), fecal indicators were positively correlated with incidence of S. enterica in well water. Bacterial taxa such as Sphingomonadaceae and Hymenobacter were positively correlated with the prevalence and population of S. enterica, and recovery of L. monocytogenes was positively correlated with the abundance of Rhizobacter and Comamonadaceae (p < 0.03). These data will support evolving mitigation strategies to reduce the risk of produce contamination by foodborne pathogens through irrigation.

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“…In our previous study, the prevalence and population density of S. enterica in irrigation tested ponds and wells were reported ( Gu et al, 2019a ).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…A most probable number (MPN) method was used to assess S. enterica concentration in collected water samples as described previously ( Luo et al, 2014 ; Gu et al, 2019a , b ).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Correlation of Salmonella enterica and Listeria monocytogenes in Irrigation Water to Environmental Factors, Fecal Indicators, and Bacterial Communities

Strawn

Ottesen

et al. 2021

Front. Microbiol.

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Evaluation of the different methods to detect Salmonella in poultry feces samples

et al. 2022

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Salmonella is one of the most common causes of food-borne outbreaks and infection worldwide. The gold standard detection method of Salmonella is cultivation. With time-consuming cultivation, there is a need to investigate rapid and accurate processes. The study evaluated different approaches to detect Salmonella in poultry feces samples. Poultry farm feces samples from 21 cities in Iran were collected from January 2016 to December 2019. Microbiological cultures, serological assays, and multiplex PCR (m-PCR) were used to detect and characterize Salmonella spp. isolates. Serological assays and m-PCR were used to determine the serogroups A, B, C1, C2, D1, E, H, and FliC. The m-PCR was used for the detection of seven Salmonella serovars and a chi-square test was performed to compare the discriminatory power of the methods. Out of 2300 poultry feces samples, 173 (7.5%) and 166 (7.2%) samples were detected as Salmonella spp. by cultivation and m-PCR, respectively. The sensitivity of the molecular method was equal to cultivation at 0.96 (CI = 95%).Assessment of H antigenic subgroups showed the same for both m-PCR and serological tests. Therefore, the matching rate of the two methods for detection of all H antigenic subgroups was 100%. Thus, the relationship between the results obtained from both methods was signi cant in the contingency table test (P < 0.01). The PCR-based approach con rmed the detection of Salmonella in a shorter period (24-36 hours) compared to the conventional microbiological approach (3-8 days).

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Spatial and temporal dynamics of microbiomes and resistomes in broiler litter stockpiles

Gupta

Avidov

Kattusamy

et al. 2021

Computational and Structural Biotechnology Journal

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Diversity and Dynamics of Salmonella enterica in Water Sources, Poultry Litters, and Field Soils Amended With Poultry Litter in a Major Agricultural Area of Virginia

Cited by 44 publications

References 33 publications

Correlation of Salmonella enterica and Listeria monocytogenes in Irrigation Water to Environmental Factors, Fecal Indicators, and Bacterial Communities

Correlation of Salmonella enterica and Listeria monocytogenes in Irrigation Water to Environmental Factors, Fecal Indicators, and Bacterial Communities

Evaluation of the different methods to detect Salmonella in poultry feces samples

Spatial and temporal dynamics of microbiomes and resistomes in broiler litter stockpiles

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