Diversity and ecology of cultivable fungi isolated from the thermal soil gradients in Deception Island, Antarctica

Figueredo, Hebert M.; Gonçalves, Vívian N.; Godinho, Valéria M.; Lopes, Daví do Vale; Oliveira, Fábio Soares de; Rosa, Luiz Henrique

doi:10.1007/s00792-019-01146-z

Cited by 16 publications

(13 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The majority of mycological studies carried out to date on Deception Island have focused on cultivable fungal diversity. Gonçalves et al 31 reported seven fungal taxa present in freshwater in Crater Lake, Held and Blanchette 32 reported 68 taxa on historic wooden structures in Whalers Bay, Figueredo et al 4 identified 17 taxa from soil samples from Fumarole Bay and de Menezes et al 33 reported 14 taxa from snow. Baeza et al 34 used culture-independent techniques to characterize fungal diversity in soils from various different sites in Antarctica, including some samples obtained from the same locations on Deception Island as studied here.…”

Section: Discussionmentioning

confidence: 99%

DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica

Rosa

Silva

Ogaki

et al. 2020

Sci Rep

View full text Add to dashboard Cite

We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Chytridiomycota. We also detected taxa belonging to the rare phyla Mucoromycota and Rozellomycota, which have been difficult to detect in Antarctica by traditional isolation methods. Cladosporium sp., Pseudogymnoascus roseus, Leotiomycetes sp. 2, Penicillium sp., Mortierella sp. 1, Mortierella sp. 2, Pseudogymnoascus appendiculatus and Pseudogymnoascus sp. were the most dominant fungi. In addition, 440,153 of the total of 1,214,875 reads detected could be classified only at the level of Fungi. In both sampling areas the DNA of opportunistic, phytopathogenic and symbiotic fungi were detected, which might have been introduced by human activities, transported by birds or wind, and/or represent resident fungi not previously reported from Antarctica. Further long-term studies are required to elucidate how biological colonization in the island may be affected by climatic changes and/or other anthropogenic influences.

show abstract

Section: Discussionmentioning

confidence: 99%

DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica

Rosa

Silva

Ogaki

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The studies regarding the fungal diversity on the island reported the same overall pattern of dominant fungal populations by isolation and meta-taxonomic approaches. Concerning the cultivable fungal community, we have detected a low diversity level as other studies have shown [16,47], with most of the isolates identified as members of the Aspergillus and Penicillium genera. These genera have been isolated regardless of the soil temperature, both in hot and cold volcanic soils of the island [16].…”

Section: Discussionmentioning

confidence: 57%

“…The most common bacterial phyla in island-soils are Proteobacteria, Bacteroidetes, and Actinobacteria [ 14 , 15 ], which are common to those observed in other regions of the continent, which communities are mainly composed of Actinobacteria, Proteobacteria, Bacteroidetes, Acidobacteria, or Deinococcus-Thermus [ 8 ]. As far as fungi are concerned, few studies have been carried out; some of them [ 16 , 17 ] demonstrated that the community is mainly composed of Ascomycetes . Nevertheless, despite these studies, the microbial community is still poorly characterized, representing a huge unknown potential of biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

Microbial Communities Present in Hydrothermal Sediments from Deception Island, Antarctica

et al. 2021

View full text Add to dashboard Cite

Deception Island is a geothermal location in Antarctica that presents active fumaroles, which confers unique characteristics to this habitat. Several studies about microbial communities in Antarctica have been carried out, nevertheless, Antarctic microbiota is still partially unknown. Here we present a multidisciplinary study about sediments obtained by deposition during 4 years in which several approaches have been considered for their characterization. First, a physicochemical characterization, using ionic chromatography and mass spectrometry for the determination of most abundant ions (chloride and sulphate) and elements (mainly silicon), was conducted. In addition, the total microbial community was studied using a metataxonomical approach, revealing a bacterial community dominated by Proteobacteria and Thaumarchaeota as the main archaeal genera and a fungal community mainly composed by Aspergillaceae. Culture-dependent studies showed low microbial diversity, only achieving the isolation of Bacillus-related species, some of them thermophilic, and the isolation of common fungi of Aspergillus or Penicillium spp. Furthermore, diatoms were detected in the sediment and characterized attending to their morphological characteristics using scanning electron microscopy. The study reveals a high influence of the physicochemical conditions in the microbial populations and their distribution, offering valuable data on the interaction between the island and water microbiota.

show abstract

“…The fungal ability to survive in a wide range of temperatures and pH could help to explain its widespread distribution and probably to the development of mycosis 3 . According to Figueredo et al, 6 Purpureocillium spp. inhibits various sites, including the Antarctica (mainly Purpureocillium sodanum ), and this genus exhibits different adaptations to extreme climates and has been recovered from different sites in this continent.…”

Section: Discussionmentioning

confidence: 99%

“…This genus belongs to the family Ophiocordycipitaceae ( Hypocreales ) and was described by Luangsa‐ard et al 4 Purpureocillium is a saprophytic, ubiquitous fungus, with a widespread distribution, frequently found in soil, and plant debris and has resilient and extremophilic nature 5,6 . The identification to the species level based on morphology is somewhat inaccurate.…”

Section: Introductionmentioning

confidence: 99%

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Calvillo‐Medina

Ponce-Angulo

Raymundo

et al. 2020

Mycoses

View full text Add to dashboard Cite

BackgroundInfectious keratitis is the main cause of preventable blindness worldwide, with about 1.5–2.0 million new cases occurring per year. This inflammatory response may be due to infections caused by bacteria, fungi, viruses or parasites. Fungal keratitis is a poorly studied health problem.ObjectivesThis study aimed to identify a new fungal species by molecular methods and to explore the possible efficacy of the three most common antifungals used in human keratitis in Mexico by performing in vitro analysis. The capacity of this pathogen to cause corneal infection in a murine model was also evaluated.MethodsThe fungal strain was isolated from a patient with a corneal ulcer. To identify the fungus, taxonomic and phylogenetic analyses (nrDNA ITS and LSU data set) were performed. An antifungal susceptibility assay for amphotericin B, itraconazole and voriconazole was carried out. The fungal isolate was used to develop a keratitis model in BALB/c mice; entire eyes and ocular tissues were preserved and processed for histopathologic examination.Results and conclusionThis fungal genus has hitherto not been reported with human keratitis in Mexico. We described a new species Purpurecillium roseum isolated from corneal infection. P roseum showed resistance to amphotericin B and itraconazole and was sensitive to voriconazole. In vivo study demonstrated that P roseum had capacity to developed corneal infection and to penetrate deeper corneal tissue. The global change in fungal infections has emphasised the need to develop better diagnostic mycology laboratories and to recognise the group of potential fungal pathogens.

show abstract

Diversity and ecology of cultivable fungi isolated from the thermal soil gradients in Deception Island, Antarctica

Cited by 16 publications

References 42 publications

DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica

DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica

Microbial Communities Present in Hydrothermal Sediments from Deception Island, Antarctica

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Contact Info

Product

Resources

About