Diversity and enzyme activity of Penicillium species associated with macroalgae in Jeju Island

Park, Myung Soo; Lee, Seobihn; Oh, Seung‐Yoon; Cho, Ga Youn; Lim, Young Woon

doi:10.1007/s12275-016-6324-0

Cited by 18 publications

(17 citation statements)

References 40 publications

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“…In general, species diversity in these genera is much higher in brown algae than either red or green algae due to different cellulose components [13,36,38,43,44]. Many Penicillium species, in particular, have been reported in macroalgae including A. clathratum [15,44].…”

Section: Discussionmentioning

confidence: 99%

Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum

et al. 2019

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Agarum clathratum, a brown macroalgae species, has recently become a serious environmental problem on the coasts of Korea. In an effort to solve this problem, fungal diversity associated with decaying A. clathratum was investigated and related β-glucosidase and endoglucanase activities were described. A total of 233 fungal strains were isolated from A . clathratum at 15 sites and identified 89 species based on morphology and a multigene analysis using the internal transcribed spacer region (ITS) and protein-coding genes including actin ( act ), β-tubulin ( benA ), calmodulin ( CaM ), and translation elongation factor ( tef1 ). Acremonium , Corollospora, and Penicillium were the dominant genera, and Acremonium fuci and Corollospora gracilis were the dominant species. Fifty-one species exhibited cellulase activity, with A. fuci , Alfaria terrestris , Hypoxylon perforatum , P. madriti , and Pleosporales sp. Five showing the highest enzyme activities. Further enzyme quantification confirmed that these species had higher cellulase activity than P . crysogenum , a fungal species described in previous studies. This study lays the groundwork for bioremediation using fungi to remove decaying seaweed from populated areas and provides important background for potential industrial applications of environmentally friendly processes.

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Section: Discussionmentioning

confidence: 99%

Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum

et al. 2019

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“…In line with our findings Fusarium. Penicillium , and Aspergillus genera have been reported to produce an extensive range of extracellular enzymes ( Kwon et al, 2007 ; Alp and Arikan, 2008 ; Park et al, 2016 ). Additionally, almost all of the assayed enzymes in the current study were known to be produced by endophytic microorganisms and are needed for both colonization of host plant and decomposition of dead tissues ( Saikkonen et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites

et al. 2017

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In this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) widely cultivated in arid zones of Tunisia. A total of 52 endophytic fungi were isolated from healthy and BLD roots of date palm trees, identified based on internal transcribed spacer-rDNA sequence analysis and shown to represent 13 species belonging to five genera. About 36.8% of isolates were shared between healthy and diseased root fungal microbiomes, whereas 18.4 and 44.7% of isolates were specific to healthy and BLD root fungal microbiomes, respectively. All isolates were able to produce at least two of the screened enzymes including amylase, cellulase, chitinase, pectinase, protease, laccase and lipase. A preliminary screening of the isolates using disk diffusion method for antibacterial activity against four Gram-positive and three Gram-negative bacteria and antifungal activities against three phytopathogenic fungi indicated that healthy and BLD root fungal microbiomes displayed interesting bioactivities against examined bacteria and broad spectrum bioactivity against fungal pathogens. Some of these endophytic fungi (17 isolates) were fermented and their extracts were evaluated for antimicrobial potential against bacterial and fungal isolates. Results revealed that fungal extracts exhibited antibacterial activities and were responsible for approximately half of antifungal activities against living fungi. These results suggest a strong link between fungal bioactivities and their secondary metabolite arsenal. EtOAc extracts of Geotrichum candidum and Thielaviopsis punctulata originating from BLD microbiome gave best results against Micrococcus luteus and Bacillus subtilis with minimum inhibitory concentration (MIC, 0.78 mg/mL) and minimum bactericidal concentration (6.25 mg/mL). G. candidum gave the best result against Rhizoctonia solani with MIC 0.78 mg/mL and minimum fungicidal concentration (MFC, 6.25 mg/mL). In conclusion, using plant microbiomes subjected to biotic stresses offers new endophytes with different bioactivities than those of healthy plants. Therefore, date palm endophytic fungi represent a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites and could be considered promising source of bioactive compounds with industrial and pharmaceutical applications.

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“…In the Med-3100 population, the fungi were mainly members of Candida. It is possible that Penicillium species contributed to the gut ecosystem as they are producers of secondary metabolites, extracellular enzymes (alginase, endoglucanase, β-glucosidase), and bioactive compounds (anti-tumor, anti-fungal, and antibacterial activity), while some species possess fatty acid synthases that fulfil numerous central biological roles in living cells [80,81]. It is possible that the different assemblages of yeast and anamorphic fungi might be the result of the environments that the different populations inhabit.…”

Section: Composition Of the Fecal Microbiota Of Sceloporus Grammicus mentioning

confidence: 99%

Dietary effects on gut microbiota of the mesquite lizard Sceloporus grammicus (Wiegmann, 1828) across different altitudes

et al. 2020

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Background: High-altitude ecosystems are extreme environments that generate specific physiological, morphological, and behavioral adaptations in ectotherms. The shifts in gut microbiota of the ectothermic hosts as an adaptation to environmental changes are still largely unknown. We investigated the food ingested and the bacterial, fungal, and protistan communities in feces of the lizard Sceloporus grammicus inhabiting an altitudinal range using metabarcoding approaches. Results: The bacterial phyla Bacteroidetes and Firmicutes, and the genera Bacteroides and Parabacteroides dominated the core fecal bacteriome, while Zygomycota and Ascomycota, and the species Basidiobolus ranarum and Basidiobolus magnus dominated the core fecal mycobiome. The diet of S. grammicus included 29 invertebrate families belonging to Arachnida, Chilopoda, and Insecta. The diversity and abundance of its diet decreased sharply at high altitudes, while the abundance of plant material and Agaricomycetes was significantly higher at the highest site. The composition of the fecal microbiota of S. grammicus was different at the three altitudes, but not between females and males. Dietary restriction in S. grammicus at 4150 m might explain the high fecal abundance of Akkermansia and Oscillopira, bacteria characteristic of long fasting periods, while low temperature favored B. magnus. A high proportion of bacterial functions were digestive in S. grammicus at 2600 and 3100, while metabolism of aminoacids, vitamins, and key intermediates of metabolic pathways were higher at 4150 m. Different assemblages of fungal species in the lizard reflect differences in the environments at different elevations. Pathogens were more prevalent at high elevations than at the low ones. Conclusions: Limiting food resources at high elevations might oblige S. grammicus to exploit other food resources and its intestinal microbiota have degradative and detoxifying capacities. Sceloporus grammicus might have acquired B. ranarum from the insects infected by the fungus, but its commensal relationship might be established by the quitinolytic capacities of B. ranarum. The mycobiome participate mainly in digestive and degradative functions while the bacteriome in digestive and metabolic functions.

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Diversity and enzyme activity of Penicillium species associated with macroalgae in Jeju Island

Cited by 18 publications

References 40 publications

Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum

Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum

Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites

Dietary effects on gut microbiota of the mesquite lizard Sceloporus grammicus (Wiegmann, 1828) across different altitudes

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