Diversity and evolutionary aspects of mucin binding (MucBP) domain repeats among Lactobacillus plantarum group strains through comparative genetic analysis

Devi, Sundru Manjulata; Halami, Prakash M.

doi:10.1016/j.syapm.2017.03.005

Cited by 20 publications

(10 citation statements)

References 40 publications

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“…Collected strains also shared several groups of adhesins, haemolysin, fibronectin-binding proteins, and penicillin acylase that actively promote bacterial colonization of mucosal tissues [ 53 , 54 ], and thus, increase the chance of bacteria being present in blood transfusable components [ 52 , 54 ]. Some adhesins such as MucBP domain-containing protein LCGL 1005 also confer to the bacteria the ability to form biofilm and to escape immune surveillance systems [ 55 ]. Furthermore, the presence of WxL domain-containing proteins (already characterized in Enterococcus faecium ) increases the L. garvieae ability to overcome the osmotic stress and to aggregate in a complex population [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate

Colagrossi

Costabile

Scutari

et al. 2022

Emerging Microbes & Infections

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Owing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as an emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambino Gesù Children’s Hospital IRCCS, Rome. Three of four patients experienced L. garvieae sepsis one day after transfusion. The L. garvieae pediatric isolates and the donor’s platelet concentrates were retrospectively collected for whole-genome sequencing and shot-gun metagenomics, respectively (Illumina HiSeq). By de novo assembly of the L. garvieae genomes, we found that all three pediatric isolates shared a 99.9% identity and were characterized by 440 common SNPs. Plasmid pUC11C (conferring virulence properties) and the temperate prophage Plg-Tb25 were detected in all three strains. Core SNP genome-based maximum likelihood and Bayesian trees confirmed their phylogenetic common origin and revealed their relationship with L. garvieae strains affecting cows and humans (bootstrap values >100 and posterior probabilities = 1.00). Bacterial reads obtained by the donor’s platelet concentrate have been profiled with MetaPhlAn2 (v.2.7.5); among these, 29.9% belonged to Firmicutes, and 5.16% to Streptococcaceae (>97% identity with L. garvieae ), confirming the presence of L. garvieae in the platelet concentrate transfusion. These data showed three episodes of sepsis for the first time due to a transfusion-associated transmission of L. garvieae in three pediatric hospitalized hematology patients. This highlights the importance to implement the screening of platelet components with new human-defined pathogens for ensuring the safety of blood supply, and more broadly, for the surveillance of emerging pathogens.

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Section: Discussionmentioning

confidence: 99%

Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate

Colagrossi

Costabile

Scutari

et al. 2022

Emerging Microbes & Infections

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“…Mub is found in the surface of the bacterial cell and can contain mucin-binding repetitive domains (MucBP) that are functionally responsible for the interaction with the mucus. 125 …”

Section: Adhesion Factors and Potential Downstream Mechanismsmentioning

confidence: 99%

The potential role of adherence factors in probiotic function in the gastrointestinal tract of adults and pediatrics: a narrative review of experimental and human studies

Gorreja

Walker

2022

Gut Microbes

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Numerous studies point to the important role of probiotic bacteria in gastrointestinal health. Probiotics act through mechanisms affecting enteric pathogens, epithelial barrier function, immune signaling, and conditioning of indigenous microbiota. Once administered, probiotics reach the gastrointestinal tract and interact with the host through bacterial surface molecules, here called adhesion factors, which are either strain- or specie-specific. Probiotic adhesion, through structural adhesion factors, is a mechanism that facilitates persistence within the gastrointestinal tract and triggers the initial host responses. Thus, an understanding of specific probiotic adhesion mechanisms could predict how specific probiotic strains elicit benefits and the potential of adherence factors as a proxy to predict probiotic function. This review summarizes the present understanding of probiotic adherence in the gastrointestinal tract. It highlights the bacterial adhesion structure types, their molecular communication with the host and the consequent impact on intestinal diseases in both adult and pediatric populations. Finally, we discuss knockout/isolation studies as direct evidence for adhesion factors conferring anti-inflammatory and pathogen inhibition properties to a probiotic. What is known : Probiotics can be used to treat clinical conditions. Probiotics improve dysbiosis and symptoms. Clinical trials may not confirm in vitro and animal studies. What is new : Adhesion structures may be important for probiotic function. Need to systematically determine physical characteristics of probiotics before selecting for clinical trials. Probiotics may be genetically engineered to add to clinical efficacy.

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“…Adhesive ability and effective colonisation in GIT is a desirable feature for probiotic bacteria because it can inhibit pathogens' growth in the lumen through competitive exclusion (London et al 2014). Various mechanisms, such as the presence of some adhesins, fimbriae, pili or cell surface proteins may be related to these phenomena (Devi and Halami 2017).…”

Section: Antimicrobial Activity Of the Selected Lb Mucosae Strainsmentioning

confidence: 99%

“…Previous studies have shown that mucus-targeting proteins or mucus-binding proteins (mub) and mucus adhesion-promoting protein (map), which were well characterised among Lactobacillus species, mediate the adherence of them to the intestinal mucosal layer (Buck et al 2005;Devi and Halami 2017;Chatterjee et al 2018). The presence of these genes strengthens the probiotic potential and mucus-binding ability of Lb.…”

Section: Antimicrobial Activity Of the Selected Lb Mucosae Strainsmentioning

confidence: 99%

In vitro assessment of safety and functional probiotic properties of Lactobacillus mucosae strains isolated from Iranian native ruminants intestine

Royan

Seighalani

Mortezaei

et al. 2021

Italian Journal of Animal Science

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The search for host isolated probiotic bacteria from animal intestine may discover new probiotic candidates with promising health properties. This study evaluated the safety and functional probiotic potential of the lactic acid bacteria (LAB) isolated from the Iranian native ruminants intestine under in vitro assays. The isolates were selected according to criteria including survivability in low pH, bile salts, pancreatic enzymes, different temperatures, NaCl concentrations, antibacterial activities, presence of adhesion genes and safety characteristics. The selected LAB were then identified to species level using 16S rRNA gene sequencing. The results showed that out of one hundred and eighty-seven LAB isolates, only six strains (NABRII50, NABRII51, NABRII52, NABRII53, NABRII54 and NABRII55) were tolerant to low pH, bile salt, pancreatin enzyme, 45 C temperature and 2% sodium chloride. The six selected isolates belonged to Lactobacillus mucosae. Two of the adhesion genes (mub and map) were detected in all strains except NABRII53. The virulence factors were observed in NABRII50, NABRII53 and NABRII55. The tetracycline resistance gene (tet (S)) was detected in NABRII55. This study was the first effort to select Lb. mucosae strains with the probiotic potential from the Iranian ruminants intestine. These results revealed that the ruminant intestinal ecosystem could be considered as a valuable origin of probiotic candidates and all the selected LAB strains except NABRII50, NABRII53 and NABRII55 could be considered as promising probiotics. HIGHLIGHTSLactobacillus mucosae strains isolated from Iranian native ruminants intestine including NABRII51, NABRII52 and NABRII54 showed probiotic potential under in vitro assays. The Lb. mucosae strains including NABRII51, NABRII52 and NABRII54 were able to survive in intestinal physiological conditions, and carried the adhesion genes, such as mub and map. In vitro biosafety assays confirmed that Lb. mucosae strains (NABRII51, NABRII52 and NABRII54) were safe to further studies.

show abstract

Diversity and evolutionary aspects of mucin binding (MucBP) domain repeats among Lactobacillus plantarum group strains through comparative genetic analysis

Cited by 20 publications

References 40 publications

Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate

Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate

The potential role of adherence factors in probiotic function in the gastrointestinal tract of adults and pediatrics: a narrative review of experimental and human studies

In vitro assessment of safety and functional probiotic properties of Lactobacillus mucosae strains isolated from Iranian native ruminants intestine

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