Diversity and expression analysis of ZIP transporters and associated metabolites under zinc and iron stress in Capsicum

Ahmad, Ilyas; Rawoof, Abdul; Priyanka,; Islam, Khushbu; Momo, John; Anju, Thattantavide; Kumar, Ajay; Ramchiary, Nirala

doi:10.1016/j.plaphy.2023.01.060

Cited by 7 publications

(2 citation statements)

References 112 publications

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“…The results indicated that the knockout of ZmHMA3 signi cantly impeded maize growth under Zn stress. Previous studies primarily focused on measuring root morphological traits, such as root length and weight (Zhang et al 2019;Ahmad et al 2023). However, in this study, a root scanner was employed to speci cally measure root diameter, root volume, and other traits, providing a more comprehensive evaluation of root morphological indicators.…”

Section: Discussionmentioning

confidence: 99%

ZmHMA3 enhances Zn stress tolerance and mediates Zn transport in Maize

Lv,

Li,

Chen

et al. 2024

Preprint

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Excessive levels of Zn have the potential to be detrimental to plant health. ZmHMA3, a member of the heavy metal ATPase (HMA) family, is responsible for the transport of Zn2+ and Cd2+ across cellular membranes. In order to investigate the role of the ZmHMA3 gene in response to Zn stress, ZmHMA3 knockout mutants were created using the CRISPR-Cas9 technique. Subsequently, gene specific expression, as well as agronomic traits, root morphology indicators, relative conductivity, antioxidant indicators, and Zn content in the leaf, root, and their subcellular components were assessed. The results demonstrated a significant accumulation of ZmHMA3 in both the leaf and root after 48 hours of Zn stress compared to the control group. The Zmhma3 knockout line exhibited decreased tolerance to toxic levels of Zn as compared to the wild type, resulting in a reduction in maize plant height, fresh weight, dry weight, water content, root morphology indicators (Length, SurfArea, AvgDiam, Rootvolume, Tips and Forks) and antioxidant enzyme activity (CAT, POD, SOD, and MDA), while also leading to an increase in membrane permeability and zinc accumulation. In conclusion, it can be inferred that ZmHMA3 likely functions as a crucial positive regulator in the response to Zn stress in maize.

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Section: Discussionmentioning

confidence: 99%

ZmHMA3 enhances Zn stress tolerance and mediates Zn transport in Maize

Lv,

Li,

Chen

et al. 2024

Preprint

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“…Saccharides are essential organic compounds in living organisms and are ubiquitous in all forms of life. They perform a multitude of critical functions in biological processes, including energy metabolism [1], differentiation [2], senescence [3], immunity [4], and resistance [5]. Saccharides can be classified according to their molecular size into monosaccharides (e.g., xylose, fructose, and glucose), oligosaccharides (e.g., turanose and melezitose), and polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

A low‐cost efficient online derivatization system for the determination of saccharides by high‐performance liquid chromatograph‐ultraviolet detector

Jiang,

Wang,

et al. 2023

J of Separation Science

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In this study, a low‐cost efficient online derivatization system was developed which allows for the detection of various types of mono‐ and oligo‐saccharides only utilizing high‐performance liquid chromatography (HPLC)‐ultraviolet detector (UV) system. In the proposed method, phenylhydrazine was used as the derivatization reagent and directly spiked in the mobile phase, allowing for the separation and detection of mono‐ and oligosaccharides in an accessible instrument system (HPLC‐UV). And the online derivatization design of the proposed method has significantly reduced the potential harm of derivatization reagents to the analysts. Furthermore, critical chromatographic parameters were optimized via the Box‐Behnken design strategy, culminating in the ideal response for saccharides. Finally, the methodology validation of the proposed method was conducted. The proposed method showed satisfactory linear ranges with acceptable correlation coefficients (R2 > 0.99), outstanding accuracy (Recovery: 95.3%–105.6%), high intra‐day precision (relative standard deviation [RSD]: 1.4%–7.1%) and inter‐day precision (RSD: 2.0%–7.4%). The robustness and ruggedness of the proposed method were proved as the recovery values in the range of 95.0%–104.6% and 95.1%–104.8% for robustness and ruggedness, respectively. These satisfactory validation results confirm the applicability and reliability of the proposed method for the analysis of saccharides in various complex real‐world samples.

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Trait-Based Approaches to Improve Nutrient Uptake Efficiency in Crops

Joshi,

Maiti

2024

Plant Functional Traits for Improving Productivity

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Diversity and expression analysis of ZIP transporters and associated metabolites under zinc and iron stress in Capsicum

Cited by 7 publications

References 112 publications

ZmHMA3 enhances Zn stress tolerance and mediates Zn transport in Maize

ZmHMA3 enhances Zn stress tolerance and mediates Zn transport in Maize

A low‐cost efficient online derivatization system for the determination of saccharides by high‐performance liquid chromatograph‐ultraviolet detector

Trait-Based Approaches to Improve Nutrient Uptake Efficiency in Crops

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