Diversity and function of membrane glycerophospholipids generated by the remodeling pathway in mammalian cells

Hishikawa, Daisuke; Hashidate, Tomomi; Shimizu, Takao; Shindou, Hideo

doi:10.1194/jlr.r046094

Cited by 302 publications

(258 citation statements)

References 133 publications

(134 reference statements)

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“…In addition, the levels of choline, PC, and many lysolipids were significantly higher, while the level of glycerol was significantly lower in ATO-treated group than that in the control group at the time point of 24 h. These observations were consistent with an earlier active membrane remodeling and a later increase in membrane breakdown in response to ATO treatment [22]. Downregulated one-carbon metabolism, fatty acid β-oxidation, and polyamine metabolism pathway One-carbon metabolism is involved in the folate and methionine cycles [23].…”

Section: Upregulated Glycerophospholipid Metabolism Pathwaysupporting

confidence: 73%

Construction of a metabolomics profile of arsenic trioxide effect in gastric carcinoma cell line SGC7901

Chen¹,

Zhang

Yang

et al. 2016

ABBS

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Arsenic trioxide (ATO) is highly effective for treating acute promyelocytic leukemia. It also holds the promise for treating solid tumors, including gastric carcinoma. However, the molecular mechanism of the effectiveness of ATO to solid tumor is still poorly understood. In this study, we chosed gastric carcinoma as an example and tried to reveal the antitumor mechanism through metabolomics. Gastric carcinoma cell line SGC7901 was treated with ATO for 6, 12, and 24 h. The global metabolite profiles were monitored by metabolomics analysis using gas chromatography (GC)/mass spectrometry (MS) and liquid chromatography/MS/MS. A total of 281 certified metabolites were reliably detected. Bioinformatics analysis showed that glycerophospholipid synthesis, one-carbon synthesis, and glutathione synthesis were affected dramatically. Other cellular functions/pathways that had been affected included inflammatory response, nicotinamide adenine dinucleotide (NAD + ), and polyamine biosynthesis pathway. The metabolomics data from this study, in combination with previous transcriptomics and proteomics data, could serve as valuable resources for the understanding of the specific antitumor mechanism of ATO treatment.

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Section: Upregulated Glycerophospholipid Metabolism Pathwaysupporting

confidence: 73%

Construction of a metabolomics profile of arsenic trioxide effect in gastric carcinoma cell line SGC7901

Chen¹,

Zhang

Yang

et al. 2016

ABBS

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“…Despite the preference of cPLA 2 IV to hydrolyze PUFAs from PC and PI, the transacylases further mediate the newly acquired n-3 PUFAs to PEs and other GPLs, which can then serve as n-3 PUFA donors for various lipases (73). Increasing the ratio of 20:5n-3/20:4n-6 in membrane GPLs is known to be manifested in an increased ratio of the classic eicosanoids PGE3/PGE2 , with well-known anti-inflammatory effects (51,74).…”

Section: Discussionmentioning

confidence: 99%

Metabolism and phospholipid assembly of polyunsaturated fatty acids in human bone marrow mesenchymal stromal cells

Tigistu-Sahle

Lampinen

Kilpinen

et al. 2017

Journal of Lipid Research

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“…The wide variety of lysoPLs that is depleted suggests that the entire lysoPL population is affected. lysoPLs are needed for PL remodeling during membrane synthesis, repair, and replacement ( 20,21 ). Because rates of DHA synthesis in the brain are insuffi cient to meet brain DHA requirements ( 6,22,23 ), it has been proposed that the brain acquires DHA as docosahexaenoyl-sn -glycero-3-phosphocholine (lyso-DHA-PC) ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Novel function of vitamin E in regulation of zebrafish (Danio rerio) brain lysophospholipids discovered using lipidomics

Choi

Leonard

Kasper

et al. 2015

Journal of Lipid Research

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This article is available online at http://www.jlr.org have used zebrafi sh to characterize the molecular consequences of vitamin E defi ciency because zebrafi sh require dietary ␣ -tocopherol, especially during embryonic development ( 2 ). Zebrafi sh also express the ␣ -tocopherol transfer protein ( ␣ -TTP), which facilitates hepatic ␣ -tocopherol secretion into the circulation in humans ( 3 ), and likely facilitates ␣ -tocopherol delivery from the yolk to the developing zebrafi sh embryo ( 4 ). Importantly, when the ␣ -TTP was knocked down in zebrafi sh embryos by 12-15 h postfertilization, the embryonic brain and eyes failed to form appropriately ( 4 ), indicating that ␣ -tocopherol is necessary for nervous system development. Further, during embryonic growth over 72 h both DHA (22:6) and arachidonic acid (AA) (20:4) decreased at faster rates in vitamin E-defi cient (E Ϫ ) compared with vitamin E-suffi cient (E+) zebrafi sh embryos ( 5 ). Moreover, adequate dietary ascorbic acid was necessary to prevent accelerated ␣ -tocopherol defi ciency and tissue damage ( 5 ).We hypothesized that the devastating effects of severe ␣ -tocopherol defi ciency in zebrafi sh embryos ( 4 ) are a result of depletion and alteration of critical brain lipids. Hypothetically, the brain, which is highly enriched in DHA yet cannot synthesize DHA to meet its needs ( 6 ), is highly susceptible to lipid peroxidation in the ␣ -tocopheroldefi cient state. It has been recognized for decades in humans that vitamin E defi ciency causes a progressive spinocerebellar ataxia ( 7 ). Moreover, Ulatowski et al. ( 8 ) have shown that vitamin E is necessary for preservation of Abstract We hypothesized that brains from vitamin E-defi cient (E ؊ ) zebrafi sh ( Danio rerio ) would undergo increased lipid peroxidation because they contain highly polyunsaturated fatty acids, thus susceptible lipids could be identifi ed. Brains from zebrafi sh fed for 9 months defi ned diets without (E ؊ ) or with (E+) added vitamin E (500 mg RRR -␣ -tocopheryl acetate per kilogram diet) were studied. Using an untargeted approach, 1-hexadecanoyl-2-docosahexaenoyl-sn -glycero-3-phosphocholine [DHA-PC 38:6, PC 16:0/22:6]was the lipid that showed the most signifi cant and greatest fold-differences between groups. DHA-PC concentrations were approximately 1/3 lower in E ؊ (4.3 ± 0.6 mg/g) compared with E+ brains (6.5 ± 0.9 mg/g, mean ± SEM, n = 10 per group, P = 0.04). Using lipidomics, 155 lipids in brain extracts were identifi ed. Only four phospholipids (PLs) were different ( P < 0.05) between groups; they were lower in E ؊ brains and contained DHA with DHA-PC 38:6 at the highest abundances. Moreover, hydroxy-DHA-PC 38:6 was increased in E ؊ brains ( P = 0.0341) supporting the hypothesis of DHA peroxidation. More striking was the depletion in E ؊ brains of nearly 60% of 19 different lysophospholipids (lysoPLs) (combined P = 0.0003), which are critical for membrane PL remodeling. Thus, E ؊ brains contained fewer DHA-PLs, more hydroxy-DHA-PCs, and fewer lysoPLs, suggesting t...

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Diversity and function of membrane glycerophospholipids generated by the remodeling pathway in mammalian cells

Cited by 302 publications

References 133 publications

Construction of a metabolomics profile of arsenic trioxide effect in gastric carcinoma cell line SGC7901

Construction of a metabolomics profile of arsenic trioxide effect in gastric carcinoma cell line SGC7901

Metabolism and phospholipid assembly of polyunsaturated fatty acids in human bone marrow mesenchymal stromal cells

Novel function of vitamin E in regulation of zebrafish (Danio rerio) brain lysophospholipids discovered using lipidomics

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